Photosynthetica 2005, 43(3):457-462 | DOI: 10.1007/s11099-005-0073-3

Photosynthetic responses of radish (Raphanus sativus var. longipinnatus) plants to infection by turnip mosaic virus

Y. P. Guo1, D. P. Guo1,*, Y. Peng1, J. S. Chen2
1 Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P. R. China
2 Institute of Bioengineering, Zhejiang University of Sciences, Hangzhou, Xiasha, P. R. China

Plant growth, chlorophyll (Chl) content, photosynthetic gas exchange, ribulose-1,5-bisphosphate carboxylase (RuBPCO) enzyme activity, and Chl fluorescence in radish (Raphanus sativus var. longipinnatus) plants were examined after turnip mosaic virus (TuMV) infection. Plant fresh mass, dry mass, Chl content, net photosynthetic rate (PN), transpiration rate (E), stomatal conductance (gs), and RuBPCO activity were significantly lower in infected plants after 5 weeks of virus infection as compared to healthy plants. The 5-week virus infection did not induce significant differences in intercellular CO2 concentration (Ci, photochemical efficiency of photosystem 2, PS2 (Fv/Fm), excitation capture efficiency of open PS2 reaction centres (Fv'/Fm'), effective quantum efficiency of photosystem 2 (ΔF/Fm'), and photochemical quenching (qP), but non-photochemical quenching (qN) and alternative electron sink (AES) were significantly enhanced. Thus the decreased plant biomass of TuMV-infected plants might be associated with the decreased photosynthetic activity mainly due to reduced RuBPCO activity.

Additional key words: chlorophyll fluorescence; gas exchange; photosystem 2; photochemical activity; ribulose-1,5-bisphosphate carboxylase, oxygenase

Received: December 16, 2004; Accepted: March 7, 2005; Published: September 1, 2005  Show citation

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Guo, Y.P., Guo, D.P., Peng, Y., & Chen, J.S. (2005). Photosynthetic responses of radish (Raphanus sativus var. longipinnatus) plants to infection by turnip mosaic virus. Photosynthetica, 43(3), 457-462. doi: 10.1007/s11099-005-0073-3
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    References

    1. Barber, J., Andersson, B.: Too much of a good thing: light can be bad for photosynthesis. - Trends biochem. Sci. 17: 61-66, 1992. Go to original source...
    2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    3. Chia, T.F., He, J.: Photosynthetic capacity in Oncidium (Orchidaceae) plants after virus eradication. - Environ. exp. Bot. 42: 11-16, 1999. Go to original source...
    4. Demmig, B., Winter, K., Kruger, A., Czygan, F.-C.: Photoinhibition and zeaxanthin formation in intact leaves. A possible role of the xanthophyll cycle in the dissipation of excess light energy. - Plant Physiol. 84: 218-224, 1987. Go to original source...
    5. Edwards, G.E., Baker, N.R.: Can CO2 assimilation in maize leaves be predicted accurately from chlorophyll fluorescence analysis? - Photosynth. Res. 37: 89-102, 1993. Go to original source...
    6. Edwardson, J.R., Christie, R.G.: Turnip Mosaic Virus, Virus Infecting Forage Legumes. Vol. II. - University of Florida, Gainesville 1986.
    7. Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and photosynthesis. - Annu. Rev. Plant Physiol. 33: 317-345, 1982. Go to original source...
    8. Foyer, C.H., Noctor, G.: Leaves in the dark see the light. - Science 284: 5414-5416, 2000. Go to original source...
    9. Friess, N., Maillet, J.: Influence of cucumber mosaic virus infection on the intraspecific competitive ability and fitness of purslane (Portulaca oleracea). - New Phytol. 132: 103-111, 1996. Go to original source...
    10. Funayama, S., Hikosaka, K., Yahara, T.: Effects of virus infection and growth irradiance on fitness components and photosynthetic properties of Eupatorium makinoi (Compositae). - Amer. J. Bot. 84: 823-829, 1997a. Go to original source...
    11. Funayama, S., Sonoike, K., Terashima, I.: Photosynthetic properties of leaves of Eupatorium makinoi infected by a gemini-virus. - Photosynth. Res. 52: 253-261, 1997b. Go to original source...
    12. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. biophys. Acta 99: 87-92, 1989. Go to original source...
    13. Guo, D.P., Guo, Y.P., Zhao, J.P., Liu, H., Peng, Y., Wang, Q.M., Chen, J.S., Rao, G.Z.: Photosynthetic rate and chlorophyll fluorescence in leaves of stem mustard (Brassica juncea var. tsatsai) after turnip mosaic virus infection. - Plant Sci. 168: 57-63, 2005. Go to original source...
    14. Guo, Y.P., Zhang, L.C. Hong, S.S., Shen, Y.K.: [Responses of gas exchange and chlorophyll fluorescence to different low temperature in Satsuma mandarin.] - Acta phytophysiol. sin. 26: 88-94, 2000. [In Chin.]
    15. Guo, Y.P., Zhou, H.F., Zeng, G.H., Zhang, L.C.: [Effects of high temperature stress on photosynthetic rate and photosystem 2 activity in Citrus.] - Chin. J. appl. Ecol. 14: 867-870, 2003. [In Chin.]
    16. Hoagland, D.R., Arnon, D.I.: The water-culture method for growing plants without soil. - University of California Agricultural Experimental Station, Berkeley 1938.
    17. Krall, J.P., Edwards, G.E.: Relationship between photosystem II activity and CO2 fixation in leaves. - Physiol. Plant. 86: 180-187, 1992. Go to original source...
    18. Leipner, J., Frachebond, Y., Stamp, P.: Effect of growing season on the photosynthetic apparatus and leaf antioxodative defenses in two maize genotypes of different chilling tolerance. - Environ. exp. Bot. 42: 129-139, 1999. Go to original source...
    19. Lichtenthaler, H.K.: Chlorophylls and carotenoids - pigments of photosynthesis biomembranes. - In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Academic Press, San Diego - New York - Berkeley - Boston - London - Sydney - Tokyo - Toronto 1987. Go to original source...
    20. Liu, X.P., Lu, W.C., Liu, Y.K., Li, J.L.: A study on TuMV strain differentiation of cruciferous vegetables from ten provinces in China. - Chin. Sci. Bull. 35: 1734-1739, 1990.
    21. Long, S.P., Bernacchi, C.J.: Gas exchange measurements, what can they tell us about the underlying limitations to photosynthesis? Procedures and sources of error. - J. exp. Bot. 54: 2393-2401, 2003. Go to original source...
    22. Loreto, F., di Marco, G., Tricoli, D., Sharkey, T.D.: Measurements of mesophyll conductance, photosynthetic electron transport and alternative electron sinks of field grown wheat leaves. - Photosynth. Res. 41: 397-403, 1994. Go to original source...
    23. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence - a practical guide. - J. exp. Bot. 51: 659-668, 2000. Go to original source...
    24. Ort, D.R., Baker, N.R.: A photoprotective role for O2 as an alternative electron sink in photosynthesis? - Curr. Opin. Plant Biol. 5: 193-198, 2002. Go to original source...
    25. Parry, M.A.J., Andralojc, P.J., Parmar, S., Keys, A.J., Habash, D.Z., Paul, M.J., Alred, R., Quick, W.P., Servaites, J.C.: Regulation of Rubisco by inhibitors in the light. - Plant Cell Environ. 20: 528-534, 1997. Go to original source...
    26. Platt, S.G., Henriques, F., Rand, L.: Effects of virus infection on the chlorophyll content, photosynthetic rate and carbon metabolism of Tolmiea menziesii. - Physiol. Plant Pathol. 16: 351-365, 1979. Go to original source...
    27. Portis, A.R., Jr.: The regulation of Rubisco by Rubisco activase. - J. exp. Bot. 46: 1285-1291, 1995. Go to original source...
    28. Ribeiro, R.V., Machado, E.C., Oliveira, R.F.: Early photosynthetic responses of sweet orange plants infected with Xylella fastidiosa. - Physiol. mol. Plant Pathol. 62: 167-173, 2003. Go to original source...
    29. Ryslava, H., Muller, K., Semoradova, S., Synkova, H., Cerovska, N.: Photosynthesis and activity of phosphoenol-pyruvate carboxylase in Nicotiana tabacum L. leaves infected by potato virus A and potato virus Y. - Photosynthetica 41: 357-363, 2003. Go to original source...
    30. Sayed, O.H.: Chlorophyll fluorescence as a tool in cereal research. - Photosynthetica 41: 321-330, 2003. Go to original source...
    31. Scheibe, R.: NADP+ malate dehydrogenase in C3-plants: regulation and role of a light-activated enzyme. - Physiol. Plant. 71: 393-400, 1987. Go to original source...
    32. Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. - In: Schulze, E.-D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 49-70. Springer-Verlag, Berlin 1994. Go to original source...
    33. Shangguan, Z.P., Shao, M.G., Dyckmans, J.: Effects of nitrogen nutrition and water deficit on net photosynthetic rate and chlorophyll fluorescence in winter wheat. - J. Plant Physiol. 156: 46-51, 2000. Go to original source...
    34. Song, L.L., Guo, Y.P. Xu, K. Zhang, L.C.: [Protective mechanism in photoinhibition of photosynthesis in Citrus unshiu leaves.] - Chin. J. appl. Ecol. 14: 47-50, 2003. [In Chin.]
    35. Swiech, R., Browning, S., Molsen, D., Stenger, D.C., Holbrook, G.P.: Photosynthesic responses of sugar beet and Nicotiana benthamiana Domin. infected with beet curly top virus. - Physiol. mol. Plant Pathol. 58: 43-52, 2001. Go to original source...
    36. Yang, C.S., Zhao, S.M., Wang, W.L., Zhu, H.Y.: [Studies on ecological control of virus diseases on Weiqing radish.] - Acta phytopathol. sin. 25: 111-114, 1998. [In Chin.]
    37. Zhang, N., Portis, A.R.: Mechanisms of light regulation of Rubisco: a specific role for the larger Rubisco activase isoform involving reductive activation by thioredoxin-f. - Proc. nat. Acad. Sci. USA 96: 9438-9443, 1999. Go to original source...
    38. Zhou, C.J., Chen, H.M.: [Study on the distribution of Chinese radish and its center of origin.] - J. Beijing agr. Univ. 17: 47-53, 1991. [In Chin.]
    39. Zhou, Y.H., Peng, Y.H., Lei, J.L., Zou, L.Y., Zheng, J.H., Yu, J.Q.: Effects of potato virus YNTN infection on gas exchange and photosystem 2 function in leaves of Solanum tuberosum L. - Photosynthetica 42: 417-423, 2004. Go to original source...

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