Photosynthetica 2010, 48(3):361-369 | DOI: 10.1007/s11099-010-0047-y

Effects of water stress on photosynthetic activity, dry mass partitioning and some associated metabolic changes in four provenances of neem (Azadirachta indica A. Juss)

Y. X. Zheng1, J. C. Wu1, F. L. Cao2, Y. P. Zhang1,*
1 Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming, China
2 Nanjing Forestry University, Nanjing, China

Chlorophyll (Chl) content, dry mass, relative water content (RWC), leaf mass per area (LMA), proline (Pro) content, malondialdehyde (MDA) content, superoxide dismutase (SOD) and peroxidase (POD) activity, P N-PAR response curves and gas exchange were studied to determine the effects of water stress on photosynthetic activity, dry mass partitioning and metabolic changes in four provenances of neem (Azadirachta indica A. Juss). The results indicated that provenance differences existed in the adaptation response to water stress that included changes to growth strategies coupled with ecophysiological and metabolic adjustments. As water stress increased, stomatal conductance (g s), net photosynthetic rate (P N), transpiration rate (E), and leaf RWC decreased while LMA increased in all provenances. Dry mass was reduced in droughted plants and the percentage increased in dry mass allocated to roots, and enzyme activities of SOD and POD were highest in neem originating from Kalyani (KA) provenance and lowest in neem originating from New Dehli (ND) provenance. In contrast, water stress increased MDA content least in KA and most in ND. Furthermore, neem originating from ND also had the greatest decrease in Chl a/b ratio while the ratio was least affected in neem originating from KA. These findings suggest neem originating from KA may have more drought resistance than neem originating from ND. The data from P N-PAR response curves are less clear. While these curves showed that drought stress increased compensation irradiance (I c) and dark respiration (R D) and decreased saturation irradiance (I s) and maximum net photosynthetic rate (P max), the extent of decline in P max was provenance dependent. P max under non-waterlimiting conditions was higher in neem originating from Jodhpur (MA) (about 14 μmol m-2 s-1) than in the other three provenances (all about 10 μmol m-2 s-1), but mild water stress had minimal effect on P max of these three provenances whereas P max of MA provenance declined to 10 μmol m-2 s-1, i.e. a similar value. However, under severe water stress P max of MA and KA provenances had declined to 40% of non-stressed values (about 6 and 4 μmol m-2 s-1, respectively) whereas the decline in P max of neem originating from Kulapachta (KU) and ND provenances was about 50% of nonstressed values (about 5 μmol m-2 s-1). These data suggest the P N responses of KU and ND provenances are most tolerant, and KA and MA least tolerant to increasing water stress, but also suggest MA provenance could be the most desired under both non-water-limiting and water-limiting conditions due to highest P max in all conditions.

Additional key words: chlorophyll contents; dry mass partitioning; maximum net photosynthetic rate; PN-PAR response curves; provenance differences

Received: January 5, 2010; Accepted: May 25, 2010; Published: September 1, 2010  Show citation

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Zheng, Y.X., Wu, J.C., Cao, F.L., & Zhang, Y.P. (2010). Effects of water stress on photosynthetic activity, dry mass partitioning and some associated metabolic changes in four provenances of neem (Azadirachta indica A. Juss). Photosynthetica, 48(3), 361-369. doi: 10.1007/s11099-010-0047-y
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    References

    1. Alberte, R.S., Thornber, J.P., Fiscus, E.L.: Water stress effects on the content and organization of chlorophyll in mesophyll and bundle sheath chloroplasts of maize. - Plant Physiol. 59: 351-353, 1977. Go to original source...
    2. Bajji, M., Lutts, S., Kinet, J.-M.: Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. - Plant Sci. 160: 669-681, 2001. Go to original source...
    3. Bor, M., Ozdemir, F., Turkan, I.: The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. - Plant Sci. 164: 77-84, 2003. Go to original source...
    4. Bowler, C., Van Montagu, M.; Inze, D.M.: Superoxide dismutase and stress tolerance. - Annu. Rev. Plant Physiol. Plant Mol. Biol. 43: 83-116, 1992. Go to original source...
    5. Boyer, J.S.: Plant productivity and environment. - Science. 218: 443-448, 1982. Go to original source...
    6. Chaves, M.M., Oliveira, M.M.: Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. - J. Exp. Bot. 55: 2365-2384, 2004. Go to original source...
    7. Clifford, S.C., Arndt, S.K., Corlett, J.E., Joshi, S., Sankhla, N., Popp, M., Jones, H.G.: The role of solute accumulation, osmotic adjustment and changes in cell wall elasticity in drought tolerance in Ziziphus mauritiana (Lamk.). - J. Exp. Bot. 49: 967-977, 1998. Go to original source...
    8. Clifford, S.C., Kadzere, I., Jones, H.G., Jackson, J.E.: Field comparisons of photosynthesis and leaf conductance in Ziziphus mauritiana and other fruit tree species in Zimbabwe. - Trees 11: 449-455, 1997. Go to original source...
    9. Colom, M.R., Vazzana, C.: Photosynthesis and PSII functionality of drought-resistant and drought-sensitive weeping love-grass plants. - Environ. Exp. Bot. 49: 135-144, 2003. Go to original source...
    10. Cornic, G.: Drought stress inhibits photosynthesis by decreasing stomatal aperture - not by affecting ATP synthesis. - Trends Plant Sci. 5: 187-188, 2000. Go to original source...
    11. Del Longo, O.T., González, C.A., Pastori, G.M., Trippi, V.S.: Antioxidant defences under hyperoxygenic and hyperosmotic conditions in leaves of two lines of maize with differential sensitivity to drought. - Plant Cell Physiol. 34: 1023-1028, 1993.
    12. Flexas, J., Escalona, J.M., Medrano, H.: Water stress induces different photosynthesis and electron transport rate regulation in grapevine. - Plant Cell Environ. 22: 39-48, 1999. Go to original source...
    13. Flexas, J., Bota, J., Escalona, J.M., Sampol, B., Medrano, H.: Effects of drought on photosynthesis in grapevines under field conditions: an evaluation of stomatal and mesophyll limitations. - Funct. Plant Biol. 29: 461-471, 2002. Go to original source...
    14. Gong, J.R., Zhang, L.X., Zhao, A.F., Bi, Y.R.: Elementary studies on physiological and biochemical anti-drought features of Artemisia ordosica. - J. Desert Res. 22: 387-392, 2002.
    15. Guo, Z.F., Lu, S.Y., Li, B.S., Li, M.Q.: Responses of rice seedlings in different drought tolerant cultivars to oxidative stress. - Acta Bot. Sin. 39: 748-752, 1997.
    16. Halliwell, B., Chirico, S.: Lipid peroxidation: its mechanism, measurement, and significance. - Amer. J. Clin. Nutr. 57: S715-S725, 1993. Go to original source...
    17. Jeon, M.W., Ali, M.B., Hahn, E.J., Paek, K.Y.: Photosynthetic pigments, morphology and leaf gas exchange during ex vitro acclimatization of micropropagated CAM Doritaenopsis plantlets under relative humidity and air temperature. - Environ. Exp. Bot. 55: 183-194, 2006. Go to original source...
    18. Kaiser, W.M., Kaiser, G., Schöner, S., Neimanis, S.: Photosynthesis under osmotic stress. Differential recovery of photosynthetic activities of stroma enzymes, intact chloroplasts, and leaf slices after exposure to high solute concentrations. - Planta 153: 430-435, 1981. Go to original source...
    19. Ketkar, A.Y., C.M. Ketkar.: Medicinal uses including pharmacology in Asia. - In: Schmutterer, H. (ed.): The Neem Tree-Source of Unique Natural Products for Integrated Pest Management, Medicine, Industry and Other Purposes. VCH Publishers Inc., New York 1995.
    20. Koul, O., M.B. Isman, C.M. Ketkar.: Properties and uses of neem, Azadirachta indica. - Can. J. Bot. 68: 1-11, 1990. Go to original source...
    21. Lawlor, D.W., Cornic, G.: Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. - Plant Cell Environ. 25: 275-294, 2002. Go to original source...
    22. Li, H.S., Sun, Q., Zhao, S.J., Zhang, W.H.: [Analytical Theory and Technology of Plant Physiology and Biochemistry.] - Higher Education Press, Beijing 2000. [In Chin.]
    23. Miyashita, K., Tanakamaru, S., Maitani, T., Kimura, K.: Recovery responses of photosynthesis, transpiration, and stomatal conductance in kidney bean following drought stress. - Environ. Exp. Bot. 53: 205-214, 2005. Go to original source...
    24. Mokhtar, G., Alexandros, B., Tasos, Z., Dalenda, B., Mokhtar, Z., Angelos, P.: Differences in abscisic acid concentration in roots and leaves of two young olive (Olea europaea L.) cultivars in response to water deficit. - Acta Physiol. Plant. 31: 825-831, 2009. Go to original source...
    25. Rodrigues, M.L., Pacheco, C.M.A., Chaves, M.M.: Soil-plant water relations, root distribution and biomass partitioning in Lupinus albus L. under drought conditions. - J. Exp. Bot. 46: 947-956, 1995. Go to original source...
    26. Seel, W.E., Hendry, G.A.F., Lee, J.A.: The combined effects of desiccation and irradiance on mosses from xeric and hydric habitats. - J. Exp. Bot. 43: 1023-1031, 1992. Go to original source...
    27. Tardieu, F., Simmonneau, T.: Variability among species of stomatal control under fluctuating soil water status and evaporative demand: modelling isohydric and anisohydric behaviours. - J. Exp. Bot. 49: 419-432, 1998. Go to original source...
    28. The Board on Science and Technology for International Development, National Research Council. Neem: A Tree for Solving Global Problems. - National Academy Press, Washington 1992.
    29. Walker, D.A.: Automated measurement of leaf photosynthetic O2 evolution as a function of photon flux density. - Phil. Trans. Roy. Soc. London B 323: 313-326, 1989. Go to original source...
    30. Wu, Z.T, Zhang, G.Y.: [Effect of abscisic acid, cytokinin and malonaldehyde on superoxide dismutase activity.] - Plant Physiol. Commun. 4: 30-32, 1990. [In Chin.]
    31. Yin, C.Y., Berninger, F., Li, C.Y.: Photosynthetic responses of Populus przewalski subjected to drought stress. - Photosynthetica 44: 62-68, 2006. Go to original source...
    32. Zhang, J.X., Kirkham M B.: Drought stress induced changes in activities of superoxide dismutase catalase and peroxidase in wheat species. - Plant Cell Physiol. 35: 785-791, 1994. Go to original source...
    33. Zhang, Y.P., Peng, X.M., Zheng, Y.X., Lai, Y.Q.: Effort on planting and product development of Azadirachta indica in Southwest China. - J. Forest. Res. 19: 252-256, 2008. Go to original source...

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