Photosynthetica 2001, 39(1):23-27 | DOI: 10.1023/A:1012479432226

Effect of Irradiance on the Thermal Stability of Thylakoid Membrane Isolated from Acclimated Wheat Leaves

A.K. Singh1, G.S. Singhal1
1 School of Life Sciences, Jawaharlal Nehru University, New Delhi, India

Thermal stability of thylakoid membranes isolated from acclimated and non-acclimated wheat (Triticum aestivum L. cv. HD 2329) leaves under irradiation was studied. Damage to the photosynthetic electron transport activity was more pronounced in thylakoid membranes isolated from non-acclimated leaves as compared to thylakoid membrane isolated from acclimated wheat leaves at 35 °C. The loss of D1 protein was faster in non-acclimated thylakoid membrane as compared to acclimated thylakoid membranes at 35 °C. However, the effect of elevated temperature on the 33 kDa protein associated with oxygen evolving complex in these two types of thylakoid membranes was minimal. Trypsin digestion of the 33 kDa protein in the thylakoid membranes isolated from control and acclimated seedlings suggested that re-organisation of 33 kDa protein occurs before its release during high temperature treatment.

Additional key words: D1 protein; oxygen evolving complex; photosynthesis; proteins; temperature; Triticum aestivum; trypsin

Published: March 1, 2001  Show citation

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Singh, A.K., & Singhal, G.S. (2001). Effect of Irradiance on the Thermal Stability of Thylakoid Membrane Isolated from Acclimated Wheat Leaves. Photosynthetica, 39(1), 23-27. doi: 10.1023/A:1012479432226
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