Photosynthetica 2024, 62(1):27-39 | DOI: 10.32615/ps.2023.038

Heterocapsa cf. bohaiensis (dinoflagellate): identification and response to nickel and iron stress revealed through chlorophyll a fluorescence

V. MÉRIOT1, 2, †, A. ROUSSEL1, 2, †, N. BRUNET3, N. CHOMERAT4, G. BILIEN4, L. LE DÉAN2, V. BERTEAUX-LECELLIER5, N. COULOMBIER6, N. LEBOUVIER1, T. JAUFFRAIS2
1 ISEA, EA7484, Campus de Nouville, University of New Caledonia, 98851 Nouméa, New Caledonia
2 Ifremer, IRD, University of New Caledonia, University of La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897 Nouméa, New Caledonia
3 CRESICA, 98851 Nouméa, New Caledonia
4 Ifremer, Littoral - LERBO, Place de la Croix, Concarneau, F-29900, France
5 CNRS, Ifremer, IRD, University of New Caledonia, University of La Réunion, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, Nouméa, New Caledonia
6 ADECAL Technopole, 1 Bis Rue Berthelot, 98846 Nouméa, New Caledonia

Metal toxicity in marine ecosystems is a growing issue owing to terrestrial runoff and anthropogenic pollution. Heterocapsa cf. bohaiensis, a newly isolated dinoflagellate from New Caledonia, was cultivated in photobioreactors operating continuously with high concentrations (10-3 M) of nickel (Ni2+) and/or iron (Fe2+) and their photosynthetic efficiency was assessed. The photosynthetic measurements indicated that H. cf. bohaiensis was tolerant to Ni2+ but sensitive to Fe2+ high concentrations. In the presence of Fe2+, maximum quantum efficiency and maximal relative electron transport rate decreased from 0.62 to 0.47 and from 156 to 102, respectively. The JIP-tests suggested a reduction of the photosynthesis in response to Fe2+ due to a disruption in the electron transport chain rather than a defect in the light absorption and trapping capacity which were on the contrary enhanced by Fe2+. These results bring new knowledge on the impact of nickel and iron on microalgae photosynthetic pathways.

Additional key words: dinoflagellate; metal stress; metallic trace elements; microalgae; PAM fluorometry; photosynthesis.

Received: July 27, 2023; Revised: September 23, 2023; Accepted: October 12, 2023; Prepublished online: January 2, 2024; Published: February 22, 2024  Show citation

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MÉRIOT, V., ROUSSEL, A., BRUNET, N., CHOMERAT, N., BILIEN, G., LE DÉAN, L., ... JAUFFRAIS, T. (2024). Heterocapsa cf. bohaiensis (dinoflagellate): identification and response to nickel and iron stress revealed through chlorophyll a fluorescence. Photosynthetica, 62(1), 27-39. doi: 10.32615/ps.2023.038
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