PT Journal
AU ZHAO, C
   CUI, J
   LI, X
   CHEN, B
   JIN, R
   YU, Z
TI Proteomic analysis of chloroplasts from chlorophyll-deficient melon mutant
SO Photosynthetica
PY 2019
BP 866
EP 874
VL 57
IS 3
DI 10.32615/ps.2019.078
WP https://ps.ueb.cas.cz/artkey/phs-201903-0019.php
DE label-free protein quantification; photosynthetic pathways; proteome analysis; qRT-PCR.
SN 03003604
AB We aimed to understand the molecular-level changes occurring in the photosynthetic metabolic network in mutant chloro-plasts. We performed comparative liquid chromatography-mass spectrometry protein profiling of wild-type (WT) and chlorophyll-deficient melon (Cucumis melo L.) mutants. We identified 390 differentially expressed proteins and 81 shared proteins varied significantly in abundance, of which 76 were upregulated and 5 were downregulated. Differentially expressed proteins were involved in the following biological processes: binding, catalytic, structural, transporter, and antioxidant activities. The mutant had 6.08-fold higher expression of glutamate-1-semialdehyde 2,1-aminomutase (GSAM), an enzyme that synthesizes the chlorophyll precursor, 5-aminolevulinic acid, and a 5.02-fold higher expression of pyridoxal biosynthesis protein, a GSAM coenzyme. An RNA recognition motif-containing protein (RRM) decreased in expression by 5.22-fold. This suggests that GSAM and RRM are particularly relevant to chlorophyll deficiency.
ER