Photosynthetica 1997, 33(11):141-145 | DOI: 10.1023/A:1006829716011

DCMU causes conformational changes of a synthetic fragment of D1 protein: A Fourier transform infrared study

Jun WANG1, LiXin ZHANG1, HouGuo LIANG1
1 Department of Biology, Lanzhou University, Lanzhou, P.R. China

A peptide ranging from residues 229 to 240 (ENESANEGYRFG) of D1 protein was synthesized by stepwise solid-phase method. Resolution enhancement techniques were combined with band curve-fitting procedures to quantitate the FTIR spectra in the amide I' region (1700-1600 cm-1). FTIR analysis showed that DCMU induced drastic structural modification with a relative decrease of the unordered structure and turns, and a substantial increase of α-helix, which indicated that a much more compact structure was formed when DCMU was applied. The results may reflect molecular information for the protective effect of DCMU against photoinhibition.

Additional key words: 3-(3,4-dichlorophenyl)-1,1-dimethylurea; peptide

Prepublished online: January 1, 1998; Published: January 1, 1997  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
WANG, J., ZHANG, L., & LIANG, H. (1997). DCMU causes conformational changes of a synthetic fragment of D1 protein: A Fourier transform infrared study. Photosynthetica, 34(1), 141-145. doi: 10.1023/A:1006829716011
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aro, E.-M., Virgin, I., Andersson, B.: Photoinhibition of Photosystem II. Inactivation, protein damage and turnover.-Biochim. biophys. Acta 1143: 113-134, 1993. Go to original source...
    2. Barber, J., Andersson, B.: Too much of a good thing: light can be bad for photosynthesis.-Trends biochem. Sci. 17: 61-66, 1992. Go to original source...
    3. Giardi, M.T., Rigoni, F., Barbato, R.: QB site modification and the binding of photosystem 2 directed inhibitors during photoinhibition.-Photosynthetica 27: 173-182, 1992.
    4. Govinjee, van Rensen, J.J.S.: Photosystem II reaction center and bicarbonate.-In: Deisenhofer, J., Norris, J.R. (ed.): The Photosynthetic Reaction Center. Vol. I. Pp. 357-389. Academic Press, San Diego New York Boston-London-Sydney-Tokyo-Toronto 1993. Go to original source...
    5. Holly, S., Laczkó, I., Fasman, G.D., Hollósi, M.: FT-IR spectroscopy indicates that Ca2+-binding to phosphorylated C-terminal fragment of the midsized neurofilament protein subunit results in β-sheet formation and β-aggregation.-Biochem. biophys. Res. Commun. 197: 755-762, 1993. Go to original source...
    6. Jansen, M.A.K., Depka, B., Trebst, A., Edelman, M.: Engagement of specific sites in the plastoquinone niche regulates degradation of the D1 protein in photosystem II.-J. biol. Chem. 268: 21246-21252, 1993. Go to original source...
    7. Kirilovsky, D., Rutherford, A.W., Etienne, A.-L.: Influence of DCMU and ferricyanide on photodamage in photosystem II.-Biochemistry 33: 3087-3095, 1994. Go to original source...
    8. Kless, H., Oren-Shamir, M., Malkin, S., McIntosh, L., Edelman, M.: The D-E region of the D1 protein is involved in multiple quinone and herbicide interactions in photosystem II.-Biochemistry 33: 10501-10507, 1994. Go to original source...
    9. Komenda, J., Masojidek, J., Boček, J., Prášil, O.: Reversible and irreversible changes of fluorescence parameters during photoinhibition in the Synechococcus elongatus cells.-Photosynthetica 28: 249-251, 1993.
    10. Michel, H., Deisenhofer, J.: Relevance of the photosynthetic reaction center from purple bacteria to the structure of photosystem II.-Biochemistry 27: 1-7, 1988. Go to original source...
    11. Nakajima, Y., Yoshida, S., Inoue, Y., Yoneyama, K., Ono, T.: Selective and specific degradation of the D1 protein induced by binding of a novel photosystem II inhibitor to the QB site.-Biochim. biophys. Acta 1230: 33-44, 1995. Go to original source...
    12. Ohad, N., Hirschberg, J.: Mutations in the D1 subunit of photosystem II distinguish between quinone and herbicide binding sites.-Plant Cell 4: 273-283, 1992. Go to original source...
    13. Prášil, O., Adir, N., Ohad, I.: Dynamics of photosystem II: Mechanism of photoinhibition and recovery processes.-In: Barber, J. (ed.): The Photosystems: Structure, Function and Molecular Biology. Pp. 295-348. Elsevier Press, Amsterdam-London-New York-Tokyo 1992. Go to original source...
    14. Susi, H., Byler, D.M.: Resolution-enhanced Fourier transform infrared spectroscopy of enzymes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 130. Pp. 290-311. Academic Press, Orlando-San Diego-New York-Austin-London-Montreal-Sydney-Tokyo-Toronto 1986. Go to original source...
    15. Trebst, A., Depka, B., Kraft, B., Johanningmeier, V.: The QB site modulates the conformation of the photosystem II reaction center peptides.-Photosynth. Res. 18: 163-177, 1988. Go to original source...
    16. Wang, J., Zhang, L.-X., Liu, Z.-L., Liang, H.-G., Yang, L., Hu, X.-Y.: A possible calcium binding site in D1 protein: A fluorescence and FTIR study of the interaction between lanthanides and a synthetic peptide.-Photosynth. Res. 44: 297-302, 1995. Go to original source...
    17. Zhang, L.-X., Wang, J., Wen, J.-Q., Liang, H.-G., Du, L.-F.: Purification and partial characterization of a protease associated with PS II particles.-Physiol. Plant. 95: 591-595, 1995. 145 Go to original source...

    Return to the content