Photosynthetica 1997, 33(14):513-522 | DOI: 10.1023/A:1006805327340

Changes in morphological and anatomical structure of cabbage (Brassica oleracea L.) outer leaves and in ultrastructure of their chloroplasts caused by an in vitro excess of nickel

J. Molas1
1 Department of Plant Physiology, Agricultural University in Lublin, Institute of Agricultural Sciences, Zamość, Poland

Morphological, anatomical and ultrastructural changes, the quantity and quality of stomata, and the chlorophyll (Chl) content in primary outer leaves of cabbage plants cv. Sława from Enkhouizen were examined. The plants were grown in agar with basic MS medium containing added nickel (as NiSO4×7 H2O) in concentrations of 0, 5, 10, and 20 g m-3 (Ni0, Ni5, Ni10, Ni20). Reduction of leaf blade area, of succulence and of leaf density, and growth of specific leaf area were noticed in plants treated with all concentrations of Ni. In Ni-treated plants the total number of stomata and open stomata decreased, and the number of defective stomata in both adaxial and abaxial side of leaves was higher. In all Ni-treated samples the volume of spongy and palisade mesophyll cells was smaller in comparison to control, and it was decreasing when the Ni concentration was increasing; at the same time, the number of mesophyll cells on the same area of cross sections of leaves was increasing. In comparison to control, the intercellular spaces of mesophyll tissue decreased in Ni10 and Ni20 plants and increased in Ni5 plants. In Ni5 plants the number of chloroplasts in mesophyll cells was higher than in the Ni0 control. Reduction of grana size and increase of number of non-appressed lamellae, which often had central arrangement, were observed. In the Ni10 and Ni20 plants, the number and size of chloroplasts decreased, and their internal membranes (especially grana) were reduced and swollen. In Ni5 plants the concentration of Chl in leaves was slightly higher than in the control; in Ni10 and Ni20 plants it was lower than in the control.

Additional key words: cell number; chlorophyll; grana; Ni; non-appressed thylakoids

Prepublished online: January 1, 1998; Published: December 1, 1997  Show citation

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Molas, J. (1997). Changes in morphological and anatomical structure of cabbage (Brassica oleracea L.) outer leaves and in ultrastructure of their chloroplasts caused by an in vitro excess of nickel. Photosynthetica, 34(4), 513-522. doi: 10.1023/A:1006805327340
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    References

    1. Abdel-Basset, R., Issa, A.A., Adam, M.S.: Chlorophyllase activity: effects of heavy metals and calcium.-Photosynthetica 31: 421-425, 1995.
    2. Arnon, D.I.: Cooper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    3. Barceló, J., Vázquez, M.D., Poschenrieder, C.: Structural and ultrastructural disorders in cadmiumtreated bush plants (Phaseolus vulgaris L.).-New Phytol 108: 37-49, 1988. Go to original source...
    4. Bergmann, W.: Ernährungsstörungen bei Kulturpflanzen. 2nd Ed.-G. Fischer Verlag, Jena 1988.
    5. Cataldo, D.A., Garland, T.R., Wildung, R.E.: Nickel in plants. I. Uptake kinetics using intact soybean seedlings.-Plant Physiol. 62: 563-565, 1978a. Go to original source...
    6. Cataldo, D.A., Garland, T.R., Wildung, R.E.: Nickel in plants. II. Distribution and chemical form in soybean plants.-Plant Physiol. 62: 566-570, 1978b. Go to original source...
    7. Čiamporová, M., Majstrík, I.: The ultrastructural response of root cells to stressful conditions.-Environ. exp. Bot. 33: 11-26, 1993. Go to original source...
    8. Clijsters, H., Van Assche, F.: Inhibition of photosynthesis by heavy metals.-Photosynth. Res. 7: 31-40, 1985. Go to original source...
    9. Csatorday, K., Walezak, C., Warden, T.: The mechanism of fatty acid inhibition in photosystem 2.-In: Biggins, J. (ed.): Progress in Photosynthesis Research. Vol. 1. Pp. 293-296. Martinus Nijhoff Publ., Dordrecht-Boston-Lancaster 1987.
    10. Goldbeck, J.H., Warden, J.T.: Interaction of linolenic acid with bound quinone molecules in Photosystem II. Time-resolved optical and electron spin resonance studies.-Biochim. biophys. Acta 767: 263-271, 1984. Go to original source...
    11. Govindjee, Downton, W.J.S., Fork, D.C., Armond, P.A.: Chlorophyll a fluorescence transient as an indicator of water potential of leaves.-Plant Sci. Lett. 20: 191-194, 1981. Go to original source...
    12. Hara, T., Sonoda, Y.: Comparison of the toxicity of heavy metals to cabbage growth.-Plant Soil 51: 127-133, 1979. Go to original source...
    13. He, J.X., Wang, J., Liang, H.G.: Effects of water stress on photochemical function and protein metabolism of photosystem II in wheat leaves.-Physiol. Plant. 93: 771-777, 1995. Go to original source...
    14. Krupa, Z., Siedlecka, A., Maksymice, W., Baszyński, T.: In vivo response of photosynthetic apparatus of Phaseolus vulgaris L. to nickel toxicity.-J. Plant Physiol. 142: 664-668, 1993. Go to original source...
    15. Maksymiec, W., Bednara, J., Baszyński, T.: Responses of runner bean plants to excess copper as a function of plant growth stages: Effects on morphology and structure of primary leaves and their chloroplast ultrastructure.-Photosynthelica 31: 427-435, 1995.
    16. Maksymiec, W., Russa, R., Urbanik-Sypniewska, T., Baszyński, T.: Changes in acyl lipid and fatty acid composition in thylakoids of copper non-tolerant spinach exposed to excess copper.-J. Plant Physiol. 140: 52-55, 1992. Go to original source...
    17. Mishra, D., Kar, M.: Nickel in plant growth and metabolism.-Bot. Rev. 40: 395-452, 1974. Go to original source...
    18. Mohanty, N., Vass, J., Demeter, S.: Impairment of photosystem 2 activity at the level of secondary quinone electron acceptor in chloroplasts treated with cobalt, nickel and zine ions.-Physiol. Plant. 76: 386-390, 1989. Go to original source...
    19. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    20. Pandolfini, T., Gabriclli, R., Vergano, O.: Ni2+ effects on lipid peroxidation and free radicals defence in Triticum aestivum L.-Physiol. Plant. 85: A 70, 1992.
    21. Sandmann, G., Böger, P.: Copper-mediated lipid peroxidation processes in photosynthetic membranes.-Plant Physiol. 66: 797-800, 1980. Go to original source...
    22. Sheoran, I.S., Aggrawal, N., Singh, R.: Effect of cadmium and nickel on in vivo carbon dioxide exchange rate of pigeon pea (Cajanus cajan L.).-Plant Soil 129: 243-249, 1990a. Go to original source...
    23. Sheoran, I.S., Singal, H.R., Singh, R.: Effect of cadmium and nickel on photosynthesis and the enzymes of the photosynthetic carbon reduction cycle in pigeon pea (Cajanus cajan L.).-Photosynth. Res. 23: 345-351, 1990b. Go to original source...
    24. Skórzyńska, E., Urbaniak-Sypniewska, T., Russa, R., Baszyński, T.: Galactolipase activity in Cd-treated runner bean plants.-J. Plant Physiol. 138: 453-459, 1991. Go to original source...
    25. Soon, Y.K., Bates, T.E., Moyer, J.R.: Land application of chemically treated sewage sludge. III. Effects on soil and plant heavy metal content.-J. environ. Qual. 9: 497-504, 1980. Go to original source...
    26. Tripathy, B.C., Bhatia, B., Mohanty, P.: Inactivation of chloroplast photosynthetic electron transport activity by Ni2+.-Biochim. biophys. Acta 638: 217-224, 1981. Go to original source...
    27. Van Assche, F., Clijsters, H.: Effect of metals on enzyme activity in plants.-Plant Cell Environ. 13: 195-206, 1990. Go to original source...
    28. Veeranjaneyulu, K., Das, V.S.R.: Intrachloroplast localization of 65Zn and 63Ni in Zn-tolerant plant (Ocimum basilicum Benth.).-J. exp. Bot. 137: 1161-1165, 1982. Go to original source...

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