Photosynthetica 1998, 35(2):169-175 | DOI: 10.1023/A:1006998420652

Differences in Cold Inactivation of Phosphoenolpyruvate Carboxylase among C4 Species: The Effect of pH and of Enzyme Concentration

G. Zervoudakis1, K. Angelopoulos1, G. Salahas1, C. D. Georgiou2
1 Department of Biology, Laboratory of Plant Physiology, University of Patra, Patra, Greece
2 Department of Biology, Section of Genetics, Cell & Developmental Biology, University of Patra, Patra, Greece

Among various C4 plants we found a wide range in the level of inactivation of phosphoenolpyruvate carboxylase (PEPC) at low temperature (0 °C). The activity of the 2-fold diluted enzyme in crude leaf extracts after 60 min incubation (compared to zero time incubation) at pH 7.5, remained above 87 % at low temperatures for the species Setaria verticillata, Portulaca oleracea, and Saccharum officinarum, and between 11 and 17 % in the species Cynodon dactylon and Atriplex halimus. The enzyme exhibited intermediate levels of inactivation (42 to 58 %) for the species Amaranthus sp., Zea mays, Salsola kali, and Digitaria sanguinalis. The enzyme activity for S. verticillata was unaffected between pH 5.7 and 8.4 during incubation at room and low temperatures. Under similar conditions, the activity of the enzyme from C. dactylon was stable between pH 5.7 and 7.0 and decreased at pH above 7.0, but for Z. mays it was enhanced between pH 5.7 and 6.8 and decreased at pH above 7.0.

Additional key words: Amaranthus sp.; Atriplex halimus; Cynodon dactylon; Digitaria sanguinalis; Portulaca oleracea; Saccharum officinarum; Salsola kali; Setaria verticillata; Zea mays

Prepublished online: June 1, 1998; Published: January 1, 1998  Show citation

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Zervoudakis, G., Angelopoulos, K., Salahas, G., & Georgiou, C.D. (1998). Differences in Cold Inactivation of Phosphoenolpyruvate Carboxylase among C4 Species: The Effect of pH and of Enzyme Concentration. Photosynthetica, 35(2), 169-175. doi: 10.1023/A:1006998420652
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