Photosynthetica 1998, 35(2):191-203 | DOI: 10.1023/A:1006902621561

Heat Emission as a Protective Mechanism against High-irradiance Stress in Sugar Maple Leaves

K. Veeranjaneyulu1, R.M. Leblanc1
1 Department of Chemistry, University of Miami, Coral Gables, s[Florida, U.S.A.

High-irradiance (HI) induced changes in heat emission, fluorescence, and photosynthetic energy storage (EST) of shade grown sugar maple (Acer saccharum Marsh.) saplings were followed using modulated photoacoustic and fluorescence spectroscopic techniques. HI-treatment at 900-4400 µmol m-2 s-1 for 15 min caused an increase in heat emission and a decrease in EST. In some leaves, HI-treatment of 900 µmol m-2 s-1 for 1 min induced a rapid increase in heat emission with a marginal decrease in EST. Parallel to the increase in heat emission, there was a decrease in fluorescence, and this phenomenon was reversible in darkness. Quenching of thermal energy dissipation and a recovery in EST were observed during the first 15 min after the HI-treatment. This down-regulation of photochemical activity and its recovery may be one of the photoprotective mechanisms in shade grown sugar maple plants. The increase in thermal energy dissipation was greater in the red absorbing long wavelength (640-700 nm) region than in the blue absorbing short wavelength region of photosynthetically active excitation radiation. The photochemical activity was affected more in short wavelengths (400-520 nm) than in the long wavelength region of the spectrum. This can be due to the migration of light-harvesting chlorophyll (Chl) a/b protein complex from photosystem (PS) 2 to PS1 and/or to the disconnection of carotenoid pool from Chls in the pigment bed of photosynthetic apparatus.

Additional key words: Acer saccharum; fluorescence; photoacoustic spectroscopy; photoinhibition; photoprotection; photosystems

Prepublished online: June 1, 1998; Published: January 1, 1998  Show citation

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Veeranjaneyulu, K., & Leblanc, R.M. (1998). Heat Emission as a Protective Mechanism against High-irradiance Stress in Sugar Maple Leaves. Photosynthetica, 35(2), 191-203. doi: 10.1023/A:1006902621561
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    References

    1. Aro, E.-M., Virgin, I., Andersson, B.: Photoinhibition of photosystem II. Inactivation, protein damage and turnover.-Biochim. biophys. Acta 1143: 113-134, 1993. Go to original source...
    2. Bilger, W., Björkman, O.: Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis.-Photosynth. Res. 25: 173-185, 1990. Go to original source...
    3. Bilger, W., Björkman, O.: Relationships among violaxanthin deepoxidation, thylakoid membrane conformation, and nonphotochemical chlorophyll fluorescence quenching in leaves of cotton (Gossypium hirsutum L.).-Planta 193: 238-246, 1994. Go to original source...
    4. Bilger, W., Björkman, O., Thayer, S.S.: Light-induced spectral absorbance changes in relation to photosynthesis and the epoxidation state of xanthophyll cycle components in cotton leaves.-Plant Physiol. 91: 542-551, 1989. Go to original source...
    5. Bults, G., Horwitz, B.A., Malkin, S., Cahen, D.: Photoacoustic measurements of photosynthetic activities in whole leaves. Photochemistry and gas exchange.-Biochim. biophys. Acta 679: 452-465, 1982. Go to original source...
    6. Buschmann, C.: Induction kinetics of heat emission before and after photoinhibition in cotyledons of Raphanus sativus.-Photosynth. Res. 14: 229-240, 1987. Go to original source...
    7. Carpentier, R., LaRue, B., Leblanc, R.M.: Photoacoustic spectroscopy of Anacystis nidulans. I. Effect of sample thickness on the photoacoustic signal.-Arch. Biochem. Biophys. 222: 403-410, 1983. Go to original source...
    8. Cleland, R.E., Melis, A., Neale, P.J.: Mechanism of photoinhibition: Photochemical reaction center inactivation in system II of chloroplasts.-Photosynth. Res. 9: 79-88, 1986. Go to original source...
    9. Critchley, C.: The molecular mechanism of photoinhibition - facts and fiction.-Aust. J. Plant Physiol. 15: 27-41, 1988. Go to original source...
    10. Demmig-Adams, B., Adams, W.W., III: Light stress and photoprotection related to the xanthophyll cycle.-In: Foyer, C.H., Mullineaux, P.M. (ed.): Causes of Photooxidative Stress and Amelioration of Defense Systems. Pp. 105-126. CRC Press, Boca Raton 1993. Go to original source...
    11. Eckert, H.-J., Geiken, B., Bernarding, J., Napiwotzki, A., Eichler, H.J., Renger, G.: Two sites of photoinhibition of the electron transfer in oxygen evolving and Tris-treated PS II membrane fragments from spinach.-Photosynth. Res. 27: 97-108, 1991. Go to original source...
    12. Fork, D.C., Bose, S., Herbert, S.K.: Radiationless transitions as a protection mechanism against photoinhibition in higher plants and a red alga.-Photosynth. Res. 10: 327-333, 1986. Go to original source...
    13. Fork, D.C., Satoh, K.: The control by state transitions of the distribution of excitation energy in photosynthesis.-Annu. Rev. Plant Physiol. 37: 335-361, 1986. Go to original source...
    14. Gilmore, A.M., Björkman, O.: Adenine nucleotides and the xanthophyll cycle in leaves. II. Comparison of the effects of CO2- and temperature-limited photosynthesis on photosystem II fluorescence quenching, the adenylate energy charge and violaxanthin de-epoxidation in cotton.-Planta 192: 537-544, 1994. Go to original source...
    15. Greer, D.H., Berry, J.A., Björkman, O.: Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature, and requirement for chloroplast-protein synthesis during recovery.-Planta 168: 253-260, 1986. Go to original source...
    16. Gruszecki, W.F., Veeranjaneyulu, K., Leblanc, R.M.: Qualitative changes in the fluorescence spectra of intact pea leaves after photoinhibition.-Biochem. Cell Biol. 69: 399-403, 1991a. Go to original source...
    17. Gruszecki, W.F., Veeranjaneyulu, K., Zelent, B., Leblanc, R.M.: Energy transfer process during senescence: fluorescence and photoacoustic studies of the intact pea leaves.-Biochim. biophys. Acta 1056: 173-180, 1991b. Go to original source...
    18. Havaux, M.: Increased thermal deactivation of excited pigments in pea leaves subjected to photoinhibitory treatments.-Plant Physiol. 89: 289-292, 1989. Go to original source...
    19. Horton, P., Lee, P.: Phosphorylation of chloroplast membrane proteins partially protects against photoinhibition.-Planta 165: 37-42, 1985. Go to original source...
    20. Horton, P., Ruban, A.V., Rees, D., Pascal, A.A., Noctor, G., Young, A.J.: Control of the light-harvesting function of chloroplast membranes by aggregation of the LHCII chlorophyll-protein complex.-FEBS Lett. 292: 1-4, 1991. Go to original source...
    21. Horton, P., Ruban, A.V., Walters, R.G.: Regulation of light harvesting in green plants. Indication by nonphotochemical quenching of chlorophyll fluorescence.-Plant Physiol. 106: 415-420, 1994. Go to original source...
    22. Jennings, R.C., Zucchelli, G., Bassi, R., Vianelli, A., Garlashi, F.M.: The relation between the minor chlorophyll spectral forms and fluorescence quenching in aggregated light harvesting chlorophyll a/b complex II.-Biochim. biophys. Acta 1184: 279-283, 1994. Go to original source...
    23. Krause, G.H.: Photoinhibition of photosynthesis. An evaluation of damaging and protective mechanisms.-Physiol. Plant. 74: 566-574, 1988. Go to original source...
    24. Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: The basics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313-349, 1991. Go to original source...
    25. Kyle, D.L., Ohad, J., Arntzen, C.J.: Membrane protein damage and repair: Selective loss of a quinone-protein function in chloroplast membranes.-Proc. nat. Acad. Sci. USA 81: 4070-4074, 1984. Go to original source...
    26. Le Gouallec, J.-L., Cornic, G., Briantias, J.-M.: Chlorophyll fluorescence and photoinhibition in a tropical rainforest understory plant.-Photosynth. Res. 27: 135-142, 1991. Go to original source...
    27. Lei, T.T., Lechowicz, M.J.: Shade adaptation and shade tolerance in saplings of three Acer species from eastern North America.-Oecologia 84: 224-228, 1990. Go to original source...
    28. Leitsch, J., Schnettger, B., Critchley, C., Krause, G.H.: Two mechanisms of recovery from photoinhibition in vivo: Reactivation of photosystem II related and unrelated to D1-protein turnover.-Planta 194: 15-21, 1994. Go to original source...
    29. Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633-662, 1994. Go to original source...
    30. Malkin, S., Canaani, O.: The use and characteristics of the photoacoustic method in the study of photosynthesis.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 493-526, 1994. Go to original source...
    31. Neale, P.J., Melis, A.: Activation of a reserve pool of photosystem II in Chlamydomonas reihardtii counteracts photoinhibition.-Plant Physiol. 92: 1196-1204, 1990. Go to original source...
    32. Ohad, I., Kyle, D.J., Arntzen, C.J.: Membrane protein damage and repair. II. Removal and replacement of inactivated 32-kilodalton polypeptides in chloroplast membranes.-J. Cell Biol. 99: 481-485. 1984. Go to original source...
    33. Pearcy, R.W.: Photosynthetic gas exchange responses of Australian tropical forest trees in canopy, gap and understory micro-environments.-Funct. Ecol. 1: 169-178, 1987. Go to original source...
    34. Pearcy, R.W.: Sunflecks and photosynthesis in plant canopies.-Annu. Rev. Plant Physiol. Plant mol. Biol. 41: 421-453, 1990. Go to original source...
    35. Pfitsch, W.A., Pearcy, R.W.: Daily carbon gain by Adenocaulon bicolor (Asteraceae), a redwood forest understory herb, in relation to its light environment.-Oecologia 80: 465-470, 1989. Go to original source...
    36. Poulet, P., Cahen, D., Malkin, S.: Photoacoustic detection of photosynthetic oxygen evolution from leaves. Quantitative analysis by phase and amplitude measurements.-Biochim. biophys. Acta 724: 433-446, 1983. Go to original source...
    37. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light.-Annu. Rev. Plant Physiol. 35: 15-44, 1984. Go to original source...
    38. Powles, S.B., Björkman, O.: Leaf movement in the shade species Oxalis oregana. II. Role in protection against injury by intense light.-Carnegie Inst. Washington Year Book 80: 63-66, 1981.
    39. Veeranjaneyulu, K., Charland, M., Charlebois, D., Leblanc, R.M.: Photoacoustic study of changes in the energy storage of photosystems I and II during state 1-state 2 transitions.-Plant Physiol. 97: 330-334, 1991. Go to original source...
    40. Veeranjaneyulu, K., Leblanc, R.M.: Action spectra of photosystems I and II in state 1 and state 2 in intact sugar maple leaves.-Plant Physiol. 104: 1209-1214, 1994. Go to original source...

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