Photosynthetica 1999, 36(1):163-170 | DOI: 10.1023/A:1007035207721

Phycobilisome from Anabaena Variabilis Kütz. and its Model Conjugates

Jiquan Zhao1, Jingquan Zhao1, Jingmin Zhang2, Lijin Jiang1
1 Institute of Photographic Chemistry, Chinese Academy of Sciences, Beijing, P.R. China
2 Institute of Laser and Spectroscopy, Zhongshan University, Guang zhou, P.R. China

The model conjugates phycocyanin-allophycocyanin (C-PC-APC) and phycoerythrocyanin-phycocyanin-allophycocyanin (PEC-C-PC-APC) were synthesized by using a heterobifunctional coupling reagent N-succinimidyl-3-(2-pyridyldithio)propionate. The rod-core complex (αβ)6 PCLRC 27(αβ)3 APCLC 8.9 and phycobilisomes were separated from Anabaena variabilis. Energy transfer features for the conjugates and the complexes were compared. The absorption and fluorescence emission spectra indicated that the linker-peptides mediate interaction of phycobiliproteins and prompt energy transfer. The energy transfer in the conjugates was detected by fluorescence emission spectra and confirmed by the addition of dithiothreitol. The conjugates may be used as models for studying the energy transfer mechanism in phycobilisomes.

Additional key words: allophycocyanin; dithiothreitol; energy transfer; N-succinimidyl-3-(2-pyridyldithio)propionate; phycocyanin; phycoerythrocyanin; rod-core complex (αβ)6PCLRC27 (αβ)3APCLC8.9

Published: June 1, 1999  Show citation

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Zhao, J., Zhao, J., Zhang, J., & Jiang, L. (1999). Phycobilisome from Anabaena Variabilis Kütz. and its Model Conjugates. Photosynthetica, 36(1-2), 163-170. doi: 10.1023/A:1007035207721
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