Photosynthetica 1999, 36(1):233 | DOI: 10.1023/A:1007099627285

Photorespiration Rate in Spinach Leaves Under Moderate NaCl Stress

C. Di Martino1, S. Delfine1, A. Alvino1, F. Loreto2
1 Dipartimento SAVA, Universita' del Molise-Campobasso, Italy
2 CNR - Istituto di Biochimica ed Ecofisiologia Vegetali, Via Salaria Km. 29.300, Monterotondo Scalo (Roma), Italy

We analyzed the effect of NaCl stress on photorespiration of spinach leaves by calculating the rate of carboxylation/oxygenation of ribulose-1,5-bisphosphate carboxylase/oxygenase, and by measuring the content of amino acids produced through photorespiration. After 20 d of NaCl stress the carboxylation rate was reduced while the oxygenation rate was not affected. The contents of serine, glycine, and alanine increased relevantly. The amount of glutamine also increased after 20 d but the amount of glutamate did not. Hence photorespiration may be stimulated under moderate NaCl stress. A relevant electron transport rate was observed under CO2-free air, which may indicate refixation of photorespiratory CO2. When NaCl accumulation proceeded for more than 20 d, photosynthesis was reduced and the content of photo-respiratory amino acids started to decrease, but the oxygenation rate did not change.

Additional key words: amino acids; carboxylation rate; electron transport rate; oxygenation rate; photosynthetic rate; Spinacia oleracea

Published: June 1, 1999  Show citation

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Di Martino, C., Delfine, S., Alvino, A., & Loreto, F. (1999). Photorespiration Rate in Spinach Leaves Under Moderate NaCl Stress. Photosynthetica, 36(1-2), 233. doi: 10.1023/A:1007099627285
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