Photosynthetica 1999, 36(4):519-524 | DOI: 10.1023/A:1007035919603

Sucrose Metabolism at Three Leaf Development Stages in Bean Plants

M. Castrillo1
1 Depto. Biologia de Organismos, Universidad Simon Bolivar, Caracas, Venezuela

Sucrose metabolism was studied at three leaf development stages in two Phaseolus vulgaris L. cultivars, Tacarigua and Montalban. The changes of enzyme activities involved in sucrose metabolism at the leaf development stages were: (1) Sink (9-11 % full leaf expansion, FLE): low total sucrose phosphate synthase (SPS) activity, and higher acid invertase (AI) activity accompanied by low sucrose synthase (SuSy) synthetic and sucrolytic activities. (2) Sink to source transition (40-47 % FLE): increase in total SPS and SuSy activities, decrease in AI activity. (3) Source (96-97 % FLE): high total SPS activity, increased SuSy activities, decreased AI activity. The hexose/sucrose ratio decreased from sink to source leaves in both bean cultivars. The neutral invertase activity was lower than that of AI; it showed an insignificant decrease during the sink-source transition.

Additional key words: acid and neutral invertases; hexose, sucrose ratio; Phaseolus vulgaris; sucrose phosphate synthase; sucrose synthase

Prepublished online: January 1, 2000; Published: December 1, 1999  Show citation

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Castrillo, M. (1999). Sucrose Metabolism at Three Leaf Development Stages in Bean Plants. Photosynthetica, 36(4), 519-524. doi: 10.1023/A:1007035919603
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    References

    1. Avigad, G.: Disaccharides.-In: Dey, P.M., Harborne, J.B. (ed.): Methods in Plant Biochemistry. Vol. 2. Pp. 111-188. Academic Press, London-San Diego-New York-Boston-Sydney-Tokyo-Toronto 1990. Go to original source...
    2. Castrillo, M., Kruger, N.J., Whatley, F.R.: Sucrose metabolism in mango fruit during ripening.-Plant Sci. 84: 45-51, 1992. Go to original source...
    3. Claussen, W., Loveys, B.R., Hawker, J.S.: Comparative investigations on the distribution of sucrose synthase activity and invertase activity within growing, mature and old leaves of some C3 and C4 species.-Physiol. Plant. 65: 275-280, 1985. Go to original source...
    4. Dale, J.E.: The control of leaf expansion.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 267-295, 1988. Go to original source...
    5. Dale, J.E., Milthorpe, F.L.: General features of the production and growth of leaves.-In: Dale, J.E., Milthorpe, F.L. (ed.): The Growth and Functioning of Leaves. Pp. 151-178. Cambridge University Press, Cambridge-London-New York-New Rochelle-Melbourne-Sydney 1983.
    6. Dickson, R.E., Larson, P.R.: Incorporation of 14C-photosynthate into major chemical fractions of source and sink leaves of cottonwood.-Plant Physiol. 56: 185-193, 1975. Go to original source...
    7. Farrar, J.F.: The whole plant: carbon partitioning during development.-In: Pollock, C.J., Farrar, J.F., Gordon, A.J. (ed.): Carbon Partitioning Within and Between Organisms. Pp. 163-179. Bios, Oxford 1992.
    8. Fellows, R.J., Geiger, D.R.: Structural and physiological changes in sugar beet leaves during sink to source conversion.-Plant Physiol. 54: 877-885, 1974. Go to original source...
    9. Geiger, D.R., Fondy, B.R.: Phloem loading and unloading: Pathways and mechanism.-What's New Plant Physiol. 11: 26-28, 1980.
    10. Giaquinta, R.: Sucrose translocation and storage in the sugar beet.-Plant Physiol. 63: 828-832, 1978. Go to original source...
    11. Greenland, A.J., Lewis, D.H.: The acid invertase of the developing third leaf of oat. I. Changes in activity of invertase and concentration of ethanol-insoluble carbohydrates.-New Phytol. 88: 265-277, 1981. Go to original source...
    12. Harn, C., Khayat, E., Daie, J.: Expression dynamics of genes encoding key carbon metabolism enzymes during sink to source transition of developing leaves.-Plant Cell Physiol. 34: 1045-1053, 1993.
    13. Ho, L.C., Baker, D.A.: Regulation of loading and unloading in long distance transport systems.-Physiol. Plant. 56: 225-230, 1982. Go to original source...
    14. King, S.P., Lunn, J.E., Furbank, R.T.: Carbohydrate content and enzyme metabolism in developing canola siliques.-Plant Physiol. 114: 153-160, 1997. Go to original source...
    15. Morris, D.A., Arthur, E.D.: An association between acid invertase activity and cell growth during leaf expansion in Phaseolus vulgaris L.-J. exp. Bot. 35: 1369-1379, 1984. Go to original source...
    16. Nguyen-Quoc, B., Krivitzky, M., Huber, S.C., Lecharny, A.: Sucrose synthase in developing maize leaves. Regulation of activity by protein level during the import to export transition.-Plant Physiol. 94: 516-523, 1990. Go to original source...
    17. Pitcher, L.H., Daie, J.: Sucrose uptake in variegated leaves.-Plant Physiol. 83: 36, 1987.
    18. Pitcher, L.H., Daie, J.: Growth and sink to source transition in developing leaves of sugarbeet.-Plant Cell Physiol. 32: 335-342, 1991. Go to original source...
    19. Pollock, C.J.: Changes in the activity of sucrose synthesizing enzymes in developing leaves of Lolium temulentum.-Plant Sci. Lett. 7: 27-31, 1976. Go to original source...
    20. Pollock, C.J., Lloyd, E.J.: The distribution of acid invertase in developing leaves of Lolium temulentum.-Planta 113: 197-200, 1977. Go to original source...
    21. Schaffer, A.A., Sagee, O., Goldschmidt, E.E., Goren, R.: Invertase and sucrose synthase activity, carbohydrate status and endogenous IAA levels during Citrus leaf development.-Physiol. Plant. 69: 151-155, 1987. Go to original source...
    22. Schmalstig, J.G., Hitz, W.D.: Contribution of sucrose synthase and invertase to the metabolism of sucrose in developing leaves.-Plant Physiol. 85: 407-412, 1987. Go to original source...
    23. ©esták, Z. (ed.): Photosynthesis During Leaf Development.-Academia, Praha; Dr W Junk Publ., Dordrecht-Boston-Lancaster 1985.
    24. Sharma, A., Sengupta, U.K.: Carbon allocation and partitioning in Vigna radiata (L.) Wilczek as affected by additional carbon gain.-Photosynthetica 34: 419-426, 1997. Go to original source...
    25. Stitt, M.: Manipulation of carbohydrate partitioning.-Curr. Opinion Biotechnol. 5: 137-143, 1994. Go to original source...
    26. Stitt, M., Wilke, I., Feil, R., Heldt, H.W.: Coarse control of sucrose-phosphate synthase in leaves: alterations of the kinetic properties in response to the rate of photosynthesis and accumulation of sucrose.-Planta 174: 217-230, 1988. Go to original source...
    27. Sturgeon, R.J.: Monosaccharides.-In: Dey, P.M., Harborne, J.B. (ed.): Methods in Plant Biochemistry. Vol. 2. Pp. 1-37. Academic Press, London-San Diego-New York-Boston-Sydney-Tokyo-Toronto 1990.
    28. Turgeon, R.: The sink-source transition in leaves.-Annu. Rev. Plant Physiol. Plant mol. Biol. 40: 119-138, 1989. Go to original source...
    29. Van Volkenburgh, E., Schmidt, M.G., Cleland, R.E.: Loss of capacity for acid induced wall-loosening as the principal cause of the cessation of cell enlargement in light grown bean leaves.-Planta 163: 500-505, 1985. Go to original source...
    30. Zimmerman, R.C., Korhrs, D.G., Steller, D.L., Alberte, R.S.: Carbon partitioning in eelgrass.-Plant Physiol. 108: 1665-1671, 1995. Go to original source...

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