Photosynthetica 1999, 36(11):87-96 | DOI: 10.1023/A:1007023030704

Comparative Anatomical, Morphological, and Physiological Parameters Controlling Photosynthesis in Two Populusxeuramericana Clones During Short-Term Osmotic Treatment

M. Courtois1, E. Boudouresque1, G. Guerrier1
1 Biologie des ligneux, EA 1207, Université d'Orléans, Orléans Cedex 2, France

The relationships between drought response and anatomical/physiological properties were assessed in two poplar clones belonging to the Aigeros section: Populusxeuramericana clone Dorskamp (drought-tolerant) and clone Luisa Avanzo (drought-sensitive). Cuttings of both clones were exposed for 12 h to 0 mM (control). 50 mM (osmotic potential -0.112 MPa), and 150 mM (-0.336 MPa) mannitol. In control, Dorskamp had smaller stomata than Luisa Avanzo, one or two layers of palisade cells, a spongy mesophyll, and high concentrations of antioxidative compounds (ascorbate, glutathione). After exposure to 50 or 150 mM mannitol, both clones closed their stomata: leaf conductance and opening of stomata decreased. When exposed to 50 mM mannitol, net photosynthetic rate (P N) and chlorophyll (Chl) and total solute contents remained stable; ribulose-1,5-bisphosphate carboxylase/-oxygenase activity, Chl synthesis and turn-over, ascorbate peroxidase and glutathione reductase activities were less affected in Dorskamp than in Luisa Avanzo. Following an exposure to 150 mM mannitol, Dorskamp exhibited higher P N and higher contents of antioxidants (ascorbate, glutathione) and antioxidative enzymes (ascorbate peroxidase, glutathione reductase) than Luisa Avanzo. Hence the drought-tolerant poplar was able to better avoid and tolerate osmotic stress.

Additional key words: active oxygen species; aminolevulinate dehydratase; antioxidants; carboxylation; carotenoids; chlorophyll; chlorophyllase; poplar; ribulose-1,5-bisphosphate carboxylase, oxygenase; stomatal conductance

Published: July 1, 1999  Show citation

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Courtois, M., Boudouresque, E., & Guerrier, G. (1999). Comparative Anatomical, Morphological, and Physiological Parameters Controlling Photosynthesis in Two Populusxeuramericana Clones During Short-Term Osmotic Treatment. Photosynthetica, 37(1), 87-96. doi: 10.1023/A:1007023030704
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