Photosynthetica 1999, 36(12):225-237 | DOI: 10.1023/A:1007187105205

Excitation-Emission Map as a Tool in Studies of Photosynthetic Pigment-Protein Complexes

Mika Keränen1, Eva-Mari Aro1, Esa Tyystjärvi1
1 Department of Biology, Laboratory Plant Physiology and Molecular Biology, Biocity A, University of Turku, Turku, Finland

Excitation-emission maps were constructed by measuring emission spectra from tobacco thylakoids and from thylakoids and intact cells of the cyanobacterium Synechocystis 6803. The measurement of such maps is greatly facilitated by the current diode-array detector technology. We show that excitation-emission maps are valuable tools for studies of the structure and energy transfer pathways in photosynthetic systems.

Additional key words: CP43 chlorophyll a binding protein; D1 protein mutant; 77 K fluorescence emission; Synechocystis 6803

Published: September 1, 1999  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Keränen, M., Aro, E., & Tyystjärvi, E. (1999). Excitation-Emission Map as a Tool in Studies of Photosynthetic Pigment-Protein Complexes. Photosynthetica, 37(2), 225-237. doi: 10.1023/A:1007187105205
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Beltrán, J.L., Ferrer, R., Guiteras, J.: Multivariate calibration of polycyclic aromatic hydrocarbon mixtures from excitation-emission fluorescence spectra.-Anal. chim. Acta 373: 311-319, 1998. Go to original source...
    3. Bennett, J., Steinback, K.E., Arntzen, C.J.: Chloroplast phosphoproteins: Regulation of excitation energy transfer by phosphorylation of thylakoid membrane polypeptides.-Proc. nat. Acad. Sci. USA 77: 5253-5257, 1980. Go to original source...
    4. Böddi, B., Ryberg, M., Sundqvist, C.: Analysis of the 77 K fluorescence emission and excitation spectra of isolated etioplast inner membranes.-J. Photochem. Photobiol. B 21: 125-133, 1993. Go to original source...
    5. Bose, S.: Chlorophyll fluorescence in green plants and energy transfer pathways in photosynthesis.-Photochem. Photobiol. 36: 725-731, 1982. Go to original source...
    6. Brody, S.S.: New excited state of chlorophyll.-Science 128: 838-839, 1958. Go to original source...
    7. Butler, W.L., Kitajima, M.: Energy transfer between photosystem II and photosystem I in chloroplasts.-Biochim. biophys. Acta 396: 72-85, 1975. Go to original source...
    8. Dau, H.: Molecular mechanisms and quantitative models of variable photosystem II fluorescence.-Photochem. Photobiol. 60: 1-12, 1994. Go to original source...
    9. Dutton, H.J., Winston, M.M., Duggar, B.M.: Chlorophyll fluorescence and energy transfer in the diatom Nitzschia closterium.-J. phys. Chem. 46: 308-313, 1943. Go to original source...
    10. Duysens, L.N.M. Transfer of Excitation Energy in Photosynthesis.-Ph.D. Thesis. State University of Utrecht, Utrecht 1952.
    11. Gantt, E., Lipschultz, C.A.: Energy transfer in phycobilisomes from phycoerythrin to allophycocyanin.-Biochim. biophys. Acta 292: 858-861, 1973. Go to original source...
    12. Gombos, Z., Wada, H., Murata, N.: The recovery of photosynthesis from low-temperature photoinhibition is accelerated by the unsaturation of membrane lipids: A mechanism of chilling tolerance.-Proc. nat. Acad. Sci. USA 91: 8787-8791, 1994. Go to original source...
    13. Govindjee: Sixty-three years since Kautsky: Chlorophyll a fluorescence.-Aust. J. Plant Physiol. 22: 131-160, 1995. Go to original source...
    14. Govindjee, Yang, L.: Structure of the red fluorescence band in chloroplasts.-J. gen. Physiol. 49: 763-780, 1966. Go to original source...
    15. Krause, G.H., Weis, E.: Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals.-Photosynth. Res. 5: 139-157, 1984. Go to original source...
    16. Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: The basics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313-349, 1991. Go to original source...
    17. Moerner, W.E.: Introduction.-In: Moerner, W.E. (ed.): Persistent Spectral Hole-Burning: Science and Applications. Pp. 1-15. Springer-Verlag, Berlin-Heidelberg 1988. Go to original source...
    18. Mullineaux, C.W., Holzwarth, A.R.: Kinetics of excitation energy transfer in the cyanobacterial phocobilisome-Photosystem II complex.-Biochim. biophys. Acta 1098: 68-78, 1991. Go to original source...
    19. Mulo, P., Tyystjärvi, T., Tyystjärvi, E., Govindjee, Mäenpää, P., Aro, E.-M.: Mutagenesis of the D-E loop of photosystem II reaction centre protein D1. Function and assembly of photosystem II.-Plant mol. Biol. 33: 1059-1071, 1997. Go to original source...
    20. Nilsson, F., Simpson, D.J., Jansson, C., Andersson, B.: Ultrastructural and biochemical characterization of a Synechocystis 6803 mutant with inactivated psbA genes.-Arch. Biochem. Biophys. 295: 340-347, 1992. Go to original source...
    21. Pätsikkä, E., Aro, E.-M., Tyystjärvi, E.: Increase in the quantum yield of photoinhibition contributes to copper toxicity in vivo.-Plant Physiol. 117: 619-627, 1998. Go to original source...
    22. Rijgersberg, C.P., Amesz, C.P., Thielen, A.P.G.M., Swager, J.A.: Fluorescence emission spectra of chloroplasts and subchloroplast preparations at low temperature.-Biochim. biophys. Acta 545: 473-482, 1979. Go to original source...
    23. Rippka, R., Deruelles, J., Waterburg, J.B., Herdman, M., Stanier, R.Y.: Genetic assignments, strain histories and properties of pure cultures of cyanobacteria.-J. gen. Microbiol. 111: 1-61, 1979. Go to original source...
    24. Sauer, K., Debreczeny, M.: Fluorescence.-In: Amesz, J., Hoff, A.J. (ed.): Biophysical Techniques in Photosynthesis. Pp. 41-61. Kluwer Academic Publ., Dordrecht-Boston-London 1996. Go to original source...
    25. Sidler, W.A.: Phycobilisome and phycobiliprotein structures.-In: Bryant, D.A. (ed.): The Molecular Biology of Cyanobacteria. Pp. 139-216. Kluwer Academic Publ., Dordrecht 1994. Go to original source...
    26. Strasser, R.J., Butler, W.L.: Fluorescence emission spectra of Photosystem I, Photosystem II and the light-harvesting chlorophyll a/b complex of higher plants.-Biochim. biophys. Acta 462: 307-313, 1977. Go to original source...
    27. Tomita, G., Rabinowitch, E.: Excitation energy transfer between pigments in photosynthetic cells.-Biophys. J. 2: 483-499, 1962. Go to original source...
    28. Trissl, H.-W., Lavergne, J.: Fluorescence induction from Photosystem II: Analytical equations for the yields of photochemistry and fluorescence derived from analysis of a model including exciton-radical pair equilibrium and restricted energy transfer between photosynthetic units.-Aust. J. Plant Physiol. 22: 183-93, 1994. Go to original source...
    29. Tyystjärvi, T., Aro, E.-M., Jansson, C., Mäenpää, P.: Changes of amino acid sequence in PEST-like area and QEEET motif affect degradation of D1 polypeptide in photosystem II.-Plant mol. Biol. 25: 517-526, 1994. Go to original source...
    30. Van Thor, J.J., Mullineaux, C. W. Matthijs, H.C.P., Hellingwerf, K.J.: Light harvesting and state transitions in cyanobacteria.-Bot. Acta 111: 430-443, 1998. Go to original source...
    31. Vermaas, W.F.J., Williams, J.G.K., Rutherford, A.W., Mathis, P., Arntzen, C.J.: Genetically engineered mutant of the cyanobacterium Synechocystis 6803 lacks the photosystem II chlorophyll-binding protein CP-47.-Proc. nat. Acad. Sci. USA 83: 9474-9477, 1986. Go to original source...
    32. Weber, G.: Enumeration of components in complex systems by fluorescence spectroscopy.-Nature 190: 27-29, 1961. Go to original source...
    33. Williams, J.G.K.: Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering methods in Synechocystis 6803.-In: Abelson, J.N., Simon, M.I. (ed.): Methods in Enzymology. Vol. 167. Pp. 766-778. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1988. Go to original source...

    Return to the content