Photosynthetica 1999, 36(12):307-323 | DOI: 10.1023/A:1007120424769

Photoadaptation in the Green Alga Spongiochloris Sp. A Three-Fluorometer Study

M. Koblížek1,2, M. Ciscato3, J. Komenda1, J. Kopencký1, P. Šiffel4, J. Masojídek1
1 Department of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences, Třeboň, Czech Republic
2 Department of Plant Physiology, University of South Bohemia, České Budějovice, Czech Republic
3 L.U.C. Department SBG Laboratory of Botany, Universitaire Campus, Diepenbeck, Belgium
4 Department of Photosynthesis, Institute of Plant Molecular Biology, Academy of Sciences, České Budějovice, Czech Republic

The dark-adapted cells of the green alga Spongiochloris sp. were exposed to "white light" of 1000 µmol(photon) m-2 s-1 for 2 h and then dark adapted for 1.5 h. Changes of photochemical activities during photoadaptation were followed by measurement of chlorophyll (Chl) fluorescence kinetics, 77 K emission spectra, photosynthetic oxygen evolution, and pigment composition. We observed a build-up of slowly-relaxing non-photochemical quenching which led to a decrease of the Fv/Fm parameter and the connectivity. In contrast to the depression of Fv/Fm (35 %) and the rise of non-photochemical quenching (∼ 1.6), we observed an increase in effective absorption cross-section (20 %), Hill reaction (30 %), photosynthetic oxygen evolution (80 %), and electron transport rate estimated from the Chl fluorescence analysis (80 %). We showed an inconsistency in the presently used interpretation schemes, and ascribe the discrepancy between the increase of effective absorption cross-section and the photosynthetic activities on one side and the effective non-photochemical quenching on the other side to the build-up of a quenching mechanism which dissipates energy in closed reaction centres. Such a type of quenching changes the ratio between thermal dissipation and fluorescence without any effect on photochemical yield. In this case the Fv/Fm ratio cannot be used as a measure of the maximum photochemical yield of PS2.

Additional key words: chlorophyll fluorescence; photochemical yield; non-photochemical quenching; connectivity; electron transport rate

Published: September 1, 1999  Show citation

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Koblížek, M., Ciscato, M., Komenda, J., Kopencký, J., Šiffel, P., & Masojídek, J. (1999). Photoadaptation in the Green Alga Spongiochloris Sp. A Three-Fluorometer Study. Photosynthetica, 37(2), 307-323. doi: 10.1023/A:1007120424769
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