Photosynthetica 1999, 36(12):325 | DOI: 10.1023/A:1007172408839

The Relation Between Changes in Non-Photochemical Quenching, Low Temperature Fluorescence Emission, and Membrane Ultrastructure Upon Binding of Polyionic Compounds and Fragments of Light-Harvesting Complex 2

D. ©tys1,2, P. ©iffel1,3, I. Hunalová1,3, J. Nebesáøová1,4
1 Laboratory of Biomembranes, Faculty of Biological Sciences, University of South Bohemia, Èeské Budìjovice, Czech Republic
2 Department of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, Trebon, Oparovický mlýn, Czech Republic
3 Institute of Molecular Biology of Plants, Academy of Sciences of the Czech Republic, Brani¹ovská 31, Èeské Budìjovice, Czech Republic
4 Institute of Parasitology, Academy of Sciences of the Czech Republic, Èeské Budìjovice, Czech Republic

Experiments were performed to distinguish some of the proposed mechanisms by which thylakoid membranes regulate the performance of photosynthetic apparatus in relation to non-photochemical quenching, qN. Aliphatic diamines were used as uncouplers of transmembrane H+ gradient as they can be transported across the membrane at the expense of hydrogen cations. Diamines did not induce changes in low-temperature fluorescence emission but induced different changes in membrane ultrastructure. Positively charged peptides did not affect membrane ultrastructure but blocked qN. In addition, they caused an increase of low temperature fluorescence emission between 710 and 720 nm. For control peptide, the maximal fluorescence increase was found at 715 nm. Fragments of light-harvesting complex 2 in their phosphorylated and non-phosphorylated form shifted the position of this increase. We believe that peptides bind to membrane surface and reduce the mobility of membrane components whose migration is needed for observation of qN. Phosphorylated and non-phosphophorylated LHC2 fragments bind to different binding sites for corresponding forms of the protein.

Additional key words: decyldiamine; ethylenediamine; peptides; membrane fragments; phosphorylation; Pisum sativum

Published: September 1, 1999  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
©tys, D., ©iffel, P., Hunalová, I., & Nebesáøová, J. (1999). The Relation Between Changes in Non-Photochemical Quenching, Low Temperature Fluorescence Emission, and Membrane Ultrastructure Upon Binding of Polyionic Compounds and Fragments of Light-Harvesting Complex 2. Photosynthetica, 37(2), 325. doi: 10.1023/A:1007172408839
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Allen, J.F.: Protein phosphorylation in regulation of photosynthesis.-Biochim. biophys. Acta 1098: 275-335, 1992. Go to original source...
    2. Barany, G., Merrifield, R.B.: Special methods in peptide synthesis.-In: Gross, E., Meienhofer, J. (ed.): The Peptides. Vol. 2. Pp. 1-284. Academic Press, New York 1980.
    3. Bennett, J.: Phosphorylation of chloroplast membrane polypeptides.-Nature 269: 344-346, 1977. Go to original source...
    4. Berg, S., Dodge, S., Krogmann, D.W., Dilley, R.A.: Chloroplast grana membrane carboxyl groups. Their involvement in membrane association.-Plant Physiol. 53: 619-627, 1974. Go to original source...
    5. Briantais, J.-M., Vernotte, C., Picaud, M., Krause, G.H.: A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts.-Biochim. biophys. Acta 548: 128-138, 1979. Go to original source...
    6. Burke, J.J., Ditto, C.L., Arntzen, C.J.: Involvement of the light-harvesting complex in cation regulation of excitation energy distribution in chloroplasts.-Arch. Biochem. Biophys. 187: 252-263, 1978. Go to original source...
    7. Cheng, L., Spangfort, M.D., Allen, J.F.: Substrate specificity and kinetics of thylakoid phosphoprotein phosphatase reaction.-Biochim. biophys. Acta 1188: 151-157, 1994. Go to original source...
    8. Crofts, A.R., Yerkes, C.T.: A molecular mechanism for qE-quenching.-FEBS Lett. 352: 265-270, 1994. Go to original source...
    9. Gilmore, A.M.: Mechanistic aspects of xantophyll cycle-dependent photoprotection in higher plant chloroplasts and leaves.-Physiol. Plant. 99: 197-200, 1997. Go to original source...
    10. Grehn, I., Fransson, B., Ragnarsson, U.: Synthesis of substrates of cyclic AMP-dependent kinase and use of their precursors for convenient preparation of phosphoserine peptides.-J. chem. Soc. Perkin Elmer I 1987: 529-535, 1987. Go to original source...
    11. Gulbrand, L., Jönsson, B., Wennerström, H., Linse, P.: Electrical double layer forces.-J. chem. Phys. 80: 2221-2228, 1984. Go to original source...
    12. Hager, A.: Die Zusammenhänge zwischen lichtinduzierten Xanthophyll-Umwandlungen und Hill Reaktion.-Ber. deutsch. bot. Gesell. 79: 94-107, 1966. Go to original source...
    13. Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: the basics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313-349, 1991. Go to original source...
    14. Krieger, A., Weis, E.: Energy-dependent quenching of chlorophyll-a-fluorescence: The involvement of proton-calcium exchange at photosystem 2.-Photosynthetica 27: 89-98, 1992.
    15. Portis, A.R., McCarty, R.E.: Quantitative relationships between phosphorylation, electron flow, and internal hydrogen ion concentrations in spinach chloroplasts.-J. biol. Chem. 251: 1610-1617, 1976. Go to original source...
    16. Rintamäkki, E., Salonen, M., Suoranta, U.M., Carlberg, I., Andersson, B., Aro, E.M.: Phosphorylation of light-harvesting complex II and photosystem II core proteins show different irradiance-dependent regulation in vivo. Application of phosphothreonine antibodies to analysis of thylakoid phosphoproteins.-J. biol. Chem. 272: 30476-30482, 1997. Go to original source...
    17. Ruban, A.V., Rees, D., Pascal, A.A., Horton, P.: Mechanism of ΔpH-dependent dissipation of absorbed excitation energy by photosynthetic membranes. II. The relationship between LHCII aggregation in vitro and qE in isolated thylakoids.-Biochim. biophys. Acta 1102: 39-44, 1992. Go to original source...
    18. Ruban, A.V., Young, A.J., Horton, P.: Dynamic properties of the minor chlorophyll a/b binding proteins of photosystem II, an in vitro model for photprotective energy dissipation in the photosynthetic membrane of green plants.-Biochemistry 35: 674-678, 1996 Go to original source...
    19. Sackmann, E.: Molecular and global structure and dynamics of membranes and lipid bilayers.-Can. J. Phys. 68: 999-1012, 1990. Go to original source...
    20. Schreiber, U.: Chlorophyll fluorescence yield changes as a tool in plant physiology. I. The measuring system.-Photosynth. Res. 4: 361-373, 1983. Go to original source...
    21. Stys, D., Stancek, M., Cheng, L., Allen, J.F.: Complex formation in plant thylakoid membranes. Competition studies on membrane protein interactions using synthetic peptide fragments.-Photosynth. Res. 44: 277-285, 1995. Go to original source...
    22. Wollenberger, L., Weibull, C., Albertsson, P.-Å.: Further characterization of the chloroplast grana margins: the non-detergent preparation of granal Photosystem I cannot reduce ferredoxin in the absence of NADP+ reduction.-Biochim. biophys. Acta 1230: 10-22, 1995. Go to original source...

    Return to the content