Photosynthetica 1999, 36(13):433 | DOI: 10.1023/A:1007111911415

Response of Photosynthesis to Radiation and Intercellular CO2 Concentration in Sun and Shade Shoots of Norway Spruce

M. Šprtová1, M.V. Marek1
1 Laboratory of Ecological Physiology of Forest Trees, Institute of Landscape Ecology, Academy of Sciences of the Czech Republic, Brno, Czech Republic

Functional differentiation of assimilation activity of sun versus shade foliage was analysed in a Norway spruce monoculture stand (age 15 years). The investigated stand density (leaf area index 8.6) and crown structure led to variation in the photosynthetically active photon flux density (PPFD) within the crowns of the sampled trees. At the saturating PPFD, the maximum rate of CO2 uptake (P Nmax) of exposed shoots (E-shoots) was 1.7 times that of the shaded shoots (S-shoots). The apparent quantum yield (α) of E-shoots was 0.9 times that of the S-shoots. A lower ability to use excess energy at high PPFD in photosynthesis was observed in the S-layer. The CO2- and PPFD-saturated rate of CO2 uptake (P Nsat) of the E-shoots was 1.12 times and the carboxylation efficiency (τ) 1.6 times that of the S-shoots. The CO2-saturated rate of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) carboxylation (VCmax) and of actual electron transport (Jamax) in the S-needles amounted to 89 and 95 % of VCmax and Jamax in the E-needles. Thus, in addition to the irradiation conditions and thus limitation by low Ja, the important limitation of photosynthesis in shade needles is due to carboxylation. This limitation of photosynthesis is accompanied by lower stomatal conductance.

Additional key words: apparent quantum yield; carboxylation efficiency; compensation irradiance; dark respiration; electron transport rate; intercellular CO2 concentration; needle area, mass ratio; Picea abies; shade, sun foliage; stomatal conductance

Prepublished online: November 1, 1999; Published: January 1, 1999  Show citation

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Šprtová, M., & Marek, M.V. (1999). Response of Photosynthesis to Radiation and Intercellular CO2 Concentration in Sun and Shade Shoots of Norway Spruce. Photosynthetica, 37(3), 433. doi: 10.1023/A:1007111911415
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    References

    1. Björkman, O.: Responses to different quantum flux densities.-In: Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H. (ed.). Physiological Plant Ecology I. Pp. 57-107. Springer-Verlag, Berlin-Heidelberg-New York 1981. Go to original source...
    2. Boardman, N.K.: Comparative photosynthesis of sun and shade plants.-Annu. Rev. Plant Physiol. 28: 355-377, 1977. Go to original source...
    3. Brooks, A., Farquhar, G.D.: Effect of temperature on the CO2/O2 specifity of ribulose-1,5-bisphosphate carboxylase/oxygenase and the rate of respiration in the light. Estimates from gas-exchange measurements on spinach.-Planta 165: 397-406, 1985. Go to original source...
    4. Caemmerer, S. von, Farquhar, G.D.: Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves.-Planta 153: 376-387, 1981. Go to original source...
    5. Demmig, B., Winter, K.: Light response of CO2 assimilation, reduction state of Q, and radiationless energy dissipation in intact leaves.-Aust. J. Plant Physiol. 15: 151-162, 1988. Go to original source...
    6. Evans, J.R.: The relationship between electron transport components and photosynthetic capacity in pea leaves grown at different irradiances.-Aust. J. Plant Physiol. 14: 157-170, 1987. Go to original source...
    7. Farquhar, G.D., Caemmerer, S. von, Berry, J.A.: A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species.-Planta 149: 78-90, 1980. Go to original source...
    8. Gnojek, A.R.: Changes in chlorophyll fluorescence and chlorophyll content in suppressed Norway spruce (Picea abies [L]. Karst.) in response to release cutting.-Trees 6: 41-47, 1992. Go to original source...
    9. Hrdlička, P.: [Changes in the content of macroelements in needles of Norway spruce during the growing season in mountains.]-Zpravodaj Beskydy 8: 49-56, 1996. [In Czech.]
    10. Kent, S.S., André, M., Cournac, I., Farinequ, J.: An integrated model for the determination of the Rubisco specifity factor, respiration in the light and other photosynthetic parameters of C3 plants in situ.-Plant Physiol. Biochem. 30: 625-637, 1992.
    11. Kirschbaum, M.U.F., Farquhar, G.D.: Temperature dependence of whole-leaf photosynthesis in Eucalyptus pauciflora Sieb. ex Spreng.-Aust. J. Plant Physiol. 11: 519-538, 1984. Go to original source...
    12. Kratochvílová, I., Janouš, D., Marek, M., Barták, M., Říha, L.: Production activity mountain cultivated Norway spruce stands under the impact of air pollution. I. General description of problems.-Ekológia (ČSSR) 4: 407-419, 1989.
    13. Lam, E., Ortiz, W., Malkin, R.: Chlorophyll a/b proteins of Photosystem I.-FEBS Lett. 168: 10-14, 1984. Go to original source...
    14. Lang, A.R.G., Xiang, Y.: Estimation of leaf area index from transmission of direct sunlight in discontinuous canopies.-Agr. Forest Meteorol. 37: 229-243, 1986. Go to original source...
    15. Leverenz, J.W.: Shade-shoot structure, photosynthetic performance in the field, and photosynthetic capacity of evergreen conifers.-Tree Physiol. 16: 109-114, 1996. Go to original source...
    16. Lewandowska, M., Hart, J.W., Jarvis, P.G.: Photosynthetic electron transport in shoots of Sitka spruce from different levels in a forest canopy.-Physiol. Plant. 41: 124-128, 1977. Go to original source...
    17. Lichtenthaler, H.K.: Chlorophylls and carotenoids - pigments of photosynthetic biomembranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987. Go to original source...
    18. Linder, S.: Chlorophyll as an indicator of nitrogen status of coniferous seedlings.-New Zeal. J. Forest Sci. 10: 166-175, 1980.
    19. Long, S.P., Hällgren, J.-E.: Measurement of CO2 assimilation by plants in the field and the laboratory.-In: Hall, D.O., Scurlock, J.M.O., Bolhàr-Nordenkampf, H.R., Leegood, R.C., Long, S.P. (ed.): Photosynthesis and Production in a Changing Environment. A Field and Laboratory Manual. Pp. 129-167. Chapman & Hall, London-Glasgow-New York-Tokyo-Melbourne-Madras 1993. Go to original source...
    20. Marek, M., Barták, M., Pirochtová, M.: Vertical topography of photosynthetic activity and crown structure in Norway spruce.-Acta Sci. nat. Acad. Sci. bohemoslov. (Brno) 22(11): 1-52, 1989.
    21. Marek, M.V., Marková, I., Kalina, J., Janoua, D.: An effect of thinning on photosynthetic characteristic of a Norway spruce canopy. II. Seasonal changes of photosynthetic pigment content.-Lesnictví-Forestry 43: 141-153, 1997.
    22. Masarovičová, E.: Gasometrical investigation into CO2 exchange of the Fagus sylvatica L. species under controlled conditions.-Biol. Práce (Bratislava) 30: 1-120, 1984.
    23. Nobel, P.S.: Photosynthetic rates of sun versus shade leaves of Hyptis emoryi Torr.-Plant Physiol. 58: 218-223, 1976. Go to original source...
    24. Pearcy, R.W., Sims, D.A.: Photosynthetic acclimation to changing light environments: scaling from the leaf to the whole plant.-In: Caldwell, M.M., Pearcy, R.W. (ed.): Exploitation of Environmental Heterogeneity by Plants. Pp. 145-174. Academic Press, San Diego-New York-Boston-London-Sydney-Tokyo-Toronto 1994. Go to original source...
    25. Pirochtová, M., Marek, M.: [A method for evaluation of the photosynthetic activity of forest trees.]-Lesnictví-Forestry 37: 399-408, 1991. [In Czech.]
    26. Priwitzer, T., Urban, O., Šprtová, M., Marek, M.V.: Chloroplastic carbon dioxide concentration in Norway spruce (Picea abies [L.] Karst.) needles relates to the position within the crown.-Photosynthetica 35: 561-571, 1998. Go to original source...
    27. Sims, D.A., Pearcy, R.W.: Photosynthetic characteristics of a tropical forest understory herb, Alocasia macrorrhiza, and a related crop species, Calocasia esculenta grown in contrasting light environment.-Oecologia 79: 53-59, 1989. Go to original source...
    28. Sims, D.A., Pearcy, R.W.: Photosynthesis and respiration in Alocasia macrorrhiza following transfers to high and low light.-Oecologia 86: 447-453, 1991. Go to original source...
    29. Troeng, E., Linder, S.: Gas exchange in a 20-year-old stand of Scots pine. II. Variation in net photosynthesis and transpiration within and between trees.-Physiol. Plant. 54: 15-23, 1982. Go to original source...
    30. Woodman, J.N.: Variation of net photosynthesis within the crown of a large forest-grown conifer.-Photosynthetica 5: 50-54, 1971.
    31. Zhang, H., Sharifi, M.R., Nobel, P.S.: Photosynthetic characteristics of sun versus shade plants of Encelia farinosa as affected by photosynthetic photon flux density, intercellular CO2 concentration, leaf water potential, and leaf temperature.-Aust. J. Plant Physiol. 22: 833-841, 1995. Go to original source...
    32. Zimmermann, R., Oren, R., Schulze, E.-D., Werk, K.S.: Performance of two Picea abies [L.] Karst. stands at different stages of decline. II. Photosynthesis and leaf conductance.-Oecologia 76: 513-518, 1988. Go to original source...

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