Photosynthetica 2000, 38(1):143-148 | DOI: 10.1023/A:1026764412164

Linear Dichroism, Fluorescence Polarization, and Path of the Thermal Deactivation of Excited Cyanobacterial (Synechococcus Elongatus) Photosystem 1 Immobilized and Oriented in Polymer Films

G.E. Białek-Bylka1, D. Sofrová2, J. Szurkowski3, R. Skwarek4, B. Sopko2, H. Manikowski1
1 Faculty of Technical Physics, Poznań, University of Technology, Poznań, Piotrowo Str. 3, Poland
2 Biochemistry Department, Faculty of Science, Charles University, Prague, Albertov, Czech Republic
3 Institute of Physics, University of Gdansk, Poland
4 Faculty of Technical Physics, Poznań, University of Technology, Poznań, Piotrowo Str. 3, Poland

Pigment-protein complexes enriched in photosystem 1 (PS1) and, for comparison, enriched in photosystem 2 (PS2) were isolated from the cyanobacterium Synechococcus elongatus Nag. f. thermalis Geitl. They were immobilized and oriented in the polyvinyl alcohol (PVA) films, and studied by linear dichroism (LD), fluorescence polarization (FP), photoacoustic spectroscopy (PAS), and polarized photoacoustic spectroscopy (PAS|| and PAS⊥). The LD signal of β-carotene in the region with maximum at 500 nm was positive in the PS1 complex. The maximum value of fluorescence polarization (FP) in the measured photosynthetic pigment region was 1.25 and was similar to higher plant values. Carotenoids exhibited different efficiencies of thermal deactivation (max. at 500 nm) in PS1 and PS2. The thermal deactivation efficiency of carotenoids in comparison with that of chlorophyll (Chl) a at its red absorbance maximum was much higher in PS1 than in PS2 complexes. Cyanobacterial complexes did not contain Chl b, interpretation of the LD, PAS, and FP results is thus easier and can be compared with PS1 and PS2 values of higher plants, especially with Chl b-less mutant values.

Additional key words: carotenoids; photoacoustic and emission spectroscopy; polarized absorption; reaction centres

Prepublished online: October 1, 2000; Published: April 1, 2000  Show citation

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Białek-Bylka, G.E., Sofrová, D., Szurkowski, J., Skwarek, R., Sopko, B., & Manikowski, H. (2000). Linear Dichroism, Fluorescence Polarization, and Path of the Thermal Deactivation of Excited Cyanobacterial (Synechococcus Elongatus) Photosystem 1 Immobilized and Oriented in Polymer Films. Photosynthetica, 38(1), 143-148. doi: 10.1023/A:1026764412164
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    References

    1. Andersson, P.O., Gillbro, T., Asato, A.E., Liu, R.S.H.: Dual singlet state emission in a series of mini-carotenes.-J. Luminescence 51: 11-20, 1992. Go to original source...
    2. Bialek-Bylka, G.E.: Model of photosynthetic pigment-protein complex.-In: Murata, N. (ed.): Research in Photosynthesis. Vol. II. Pp. 849-852. Kluwer Academic Publ., Dordrecht-Boston-London 1992.
    3. Bialek-Bylka, G.E., Brown, J.S.: Spectroscopy of native chlorophyll-protein complexes embedded in polyvinyl alcohol films.-Photobiochem. Photobiophys. 13: 63-71, 1986.
    4. Bialek-Bylka, G.E., Brown, J.S., Manikowski, H.: Active pigment-protein photosystem 1 complex in artificial matrix.-Photosynthetica 21: 182-184, 1987.
    5. Bialek-Bylka, G.E., Fujii, R., Chen, C.-H., Oh-oka, H., Kamiesu, A., Satoh, K., Koike, H., Koyama, Y.: 15-Cis-carotenoids found in the reaction center of green sulfur bacterium Chlorobium tepidum in the Photosystem I reaction center of a cyanobacterium Synechococcus vulcanus.-Photosynth. Res. 58:135-142, 1998. Go to original source...
    6. Bialek-Bylka, G.E., Hiyama, T., Yumoto, K., Koyama, Y.: 15-Cis-β-carotene found in the reaction center of spinach Photosystem I.-Photosynth. Res. 49: 245-250, 1996. Go to original source...
    7. Bialek-Bylka, G.E., Shkuropatov, A.Ya., Kadoshnikov, S.I., Frąckowiak, D.: Excitation energy transfer between β-carotene and chlorophyll a in various systems.-Photosynth. Res. 3: 241-254, 1982. Go to original source...
    8. Bialek-Bylka, G.E., Sofrova, D., Skwarek, R., Kucera, T., Manikowski, H.: Energy transfer in cyanobacterial (Synechococcus elongatus) photosystem I and II.-Curr. Topics Biophys. 21: 3-7, 1997.
    9. Bialek-Bylka, G.E., Tomo, T., Satoh, K., Koyama, Y.: 15-cis-β-carotene found in the reaction center of spinach photosystem II.-FEBS Lett. 363: 137-140, 1995. Go to original source...
    10. Biggins, J., Svejkovský, J.: Linear dichroism of microalgae, developing thylakoids and isolated pigment-protein complexes in stretched poly(vinyl alcohol) films at 77 K-Biochim. biophys. Acta 592: 565-576, 1980. Go to original source...
    11. Breton, J., Vermeglio, A.: Orientation of photosynthetic pigments in vivo.-In: Govindjee (ed.): Photosynthesis. Vol. I. Pp. 153-194. Acad. Press, New York-London-Paris-San Diego-San Francisco-São Paulo-Sydney-Tokyo-Toronto 1982. Go to original source...
    12. Brody, S.S.: The position of carotene in the D-1/D-2 sub-core complex of photosystem II.-Z. Naturforsch. 43 C: 226-230, 1988. Go to original source...
    13. Brown, J.S.: A new evolution of chlorophyll absorption in photosynthetic membranes.-Photosynth. Res. 4: 375-383, 1983. Go to original source...
    14. Burton, K.S., Chow, W.S., Jordan, B.R.: The chlorophyll b-containing CP1 complexes of spinach chloroplasts.-Physiol. vég. 22: 793-800, 1984.
    15. Cogdell, R.J.: Carotenoids in photosynthesis.-Phil. Trans. roy. Soc. London B 284: 569-579, 1978. Go to original source...
    16. Cogdell, R.J., Frank, H.A.: How carotenoids function in photosynthetic bacteria.-Biochim. biophys. Acta 895: 63-79, 1987. Go to original source...
    17. Cosgrove, S.A., Guite, M.A., Burnell, T.B., Christensen R.L.: Electronic relaxation in long polyenes.-J. phys. Chem. 94: 8118-8124, 1990. Go to original source...
    18. DeCoster, B., Christensen, R.L., Gebhard, R., Lugtenburg, J., Farhoosh, R., Frank, H.A.: Low-lying electronic states of carotenoids.-Biochim. biophys. Acta 1102: 107-114, 1992. Go to original source...
    19. Dexter, D.L.: A theory of sensitized luminescence in solids.-J. chem. Phys. 21: 836-850, 1953. Go to original source...
    20. Fiksiński, K., Frąckowiak, D.: Comparison of various films used in biophysical investigations as anisotropic matrixes.-Spectrosc. Lett. 13: 873-889, 1980. Go to original source...
    21. Frank, H.A., Cogdell, R.J.: The photochemistry and function of carotenoids in photosynthesis.-In: Young, A., Britton, G. (ed.): Carotenoids in Photosynthesis. Pp. 252-326. Chapman &; Hall, London-Glasgow-New York-Tokyo-Melbourne-Madras 1993. Go to original source...
    22. Frank, H.A., Cogdell, R.J.: Carotenoids in photosynthesis.-Photochem. Photobiol. 63: 257-264, 1996. Go to original source...
    23. Gillbro, T., Cogdell, R.J.: Carotenoid fluorescence.-Chem. Phys. Lett. 158: 312-316, 1989. Go to original source...
    24. Guikema, J.A., Sherman, L.A.: Electrophoretic profiles of cyanobacterial membrane polypeptide showing heme-dependent peroxidase activity.-Biochim. biophys. Acta 637: 189-201, 1981. Go to original source...
    25. Hashimoto, H., Koyama, Y.: The 21Ag-state of carotenoid bound to spinach chloroplasts as revealed by picosecond transient Raman spectroscopy.-Biochim. biophys. Acta 1017: 181-186, 1990. Go to original source...
    26. Junge, W., Schaffernicht, H., Nelson, N.: On the mutual orientation of pigments in Photosystem I particles from green plants.-Biochim. biophys. Acta 462: 73-85, 1977. Go to original source...
    27. Katoh, T., Nagashima, U., Mimuro, M.: Fluorescence properties of the allenic carotenoid fucoxanthin. Implication for energy transfer in photosynthetic systems.-Photosynth. Res. 27: 221-226, 1991. Go to original source...
    28. Koyama, Y.: Structures and functions of carotenoids in photosynthetic systems.-Photochem. Photobiol. B 9: 265-280, 1991.
    29. Lichtenthaler, H.K.: Chlorophylls and carotenoids - pigments of photosynthetic membranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Acad. Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987. Go to original source...
    30. Mathis, P., Schenck, C.: The function of carotenoids in photosynthesis.-In: Britton, G., Goodwin, T.W. (ed.): Carotenoid Chemistry and Biochemistry. Pp. 339-351. Pergamon Press, Oxford-New York-Toronto-Sydney-Paris-Franfurt 1982. Go to original source...
    31. Mimuro, M., Katoh, T.: Carotenoids in photosynthesis: absorption, transfer and dissipation of light energy.-Pure appl. Chem. 63: 123-130, 1991. Go to original source...
    32. Mullet, J.E., Burke, J.J., Arntzen, C.J.: A developmental study of photosystem I peripherial chlorophyll proteins.-Plant Physiol. 65: 823-827, 1980. Go to original source...
    33. Noguchi, T., Kolaczkowski, S., Arbour, C., Aramaki, S., Atkinson, G.H., Hayashi, H., Tasumi, M.: Resonance Raman spectrum of the excited 21Ag state of β-carotene.-Photochem. Photobiol. 50: 603-609, 1989. Go to original source...
    34. Rosencweig, A.: Photoacoustic Spectroscopy.-Wiley, New York 1980.
    35. Satoh, K.: Isolation and properties of the photosystem II reaction center.-In: Deisenhofer, J., Norris, J.R. (ed.).: The Photosynthetic Reaction Center. Vol. 1. Pp. 289-318. Acad. Press, San Diego-New York-Boston-London-Sydney-Tokyo-Toronto 1993. Go to original source...
    36. Shreve, A.P., Trautman, J.K., Owens, T.G., Albrecht, A.C.: Determination of the S2 lifetime of β-carotene.-Chem. Phys. Lett. 178: 89-96, 1991. Go to original source...
    37. Siefermann-Harms, D.: Carotenoids in photosynthesis. I. Location in photosynthetic membranes and light-harvesting function.-Biochim. biophys. Acta 811: 325-355, 1985. Go to original source...
    38. Siefermann-Harms, D.: The light-harvesting and protective functions of carotenoids in photosynthetic membranes.-Physiol. Plant. 69: 561-568, 1987. Go to original source...
    39. Siodmiak, J., Frąckowiak, D.: Polarization of fluorescence of riboflavin in anisotropic medium.-Photochem. Photobiol. 16: 173-182, 1972. Go to original source...
    40. Sofrová, D., Kučera, T., Hladík, J.: Oligomeric state of cyanobacterial photosystem II.-In: Murata, N. (ed.): Research in Photosynthesis. Vol. I. Pp. 585-588. Kluwer Academic Publ., Dordrecht-Boston-London 1992.
    41. Szitó, T., Zimányi, L., Faludi-Dániel, A.: Fluorescence polarization spectra of granal and stromal membranes treated with linolenic acid. Orientation of the Photosystem I core complex within the membrane.-Biochim. biophys. Acta 808: 428-436, 1985. Go to original source...
    42. Takahashi, Y., Koike, H., Katoh, S.: Multiple forms of chlorophyll-protein complexes from a thermophilic cyanobacterium Synechococcus sp.-Arch. Biochem. Biophys. 219: 209-218, 1982. Go to original source...
    43. Tapie, P., Choquet, Y., Breton, J., Delepelaire, P., Wollman, F.-A.: Orientation of photosystem-I pigments. Investigation by low-temperature linear dichroism and polarized fluorescence emission.-Biochim. biophys. Acta 767: 57-69, 1984. Go to original source...
    44. Tukaj, Z., Szurkowski, J.: Photoacoustic spectra affected by fuel oil in the chlorococcal alga Scenedesmus armatus.-Acta Physiol. Plant. 15: 219-226, 1993.
    45. Van Grondelle, R.: Excitation energy transfer, trapping and annihilation in photosynthetic systems.-Biochim. biophys. Acta 811: 147-195, 1985. Go to original source...
    46. Wasielewski, M.R., Kispert, L.D.: Direct measurement of the lowest excited singlet-state lifetime of all trans-β-carotene and related carotenoids.-Chem. Phys. Lett. 128: 238-243, 1986. Go to original source...
    47. Young, A., Britton, G. (ed.): Carotenoids in photosynthesis.-Pp. 253-452. Chapman &; Hall, London-Glasgow-New York-Tokyo-Melbourne-Madras 1993.

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