Photosynthetica 2000, 38(4):493-496 | DOI: 10.1023/A:1012492919852

Inner Structure of Intact Chloroplasts Observed by a Low Temperature Laser Scanning Microscope

F. Vácha1,2, M. Vácha3,4, L. Bumba1, K. Hashizume3, T. Tani3
1 Institute of Plant Molecular Biology, Academy of Science of the Czech Republic, Budìjovice, Czech Republic
2 Faculty of Biological Sciences, University of South Bohemia, Budìjovice, Czech Republic
3 Department of Applied Physics, Tokyo University of Agriculture and Technology, Tokyo, Japan
4 Venture Business Laboratory, Tokyo University of Agriculture and Technology, Tokyo, Japan

Inner structure of isolated intact chloroplasts was observed for the first time by a method of laser scanning microscopy at the temperature of liquid nitrogen at 77 K. The microscope, based on gradient index optics, has a maximum resolution of 440 nm at the wavelength of 650 nm. Chloroplasts were excited into the Q-band of chlorophyll b by a krypton laser line at 647.6 nm and fluorescence was detected using two different interference filters. The 680 nm interference filter detects the regions where photosystem (PS) 2 mainly occurs, the 730 nm interference filter detects domains with predominant location of PS1. Since PS1 occurs mainly in stroma lamellae, whereas PS2 occurs mainly in grana regions we were able to view the structure of thylakoid membrane in isolated intact chloroplast that is the closest to in vivo state.

Additional key words: chlorophyll fluorescence; grana; photosystems 1 and 2; Spinacia; thylakoid

Prepublished online: August 1, 2000; Published: November 1, 2000  Show citation

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Vácha, F., Vácha, M., Bumba, L., Hashizume, K., & Tani, T. (2000). Inner Structure of Intact Chloroplasts Observed by a Low Temperature Laser Scanning Microscope. Photosynthetica, 38(4), 493-496. doi: 10.1023/A:1012492919852
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    References

    1. Brangeon, J., Mustárdy, L.: The ontogenetic assembly of intrachloroplastic lamellae viewed in 3-dimension.-Biol. cell. 36: 71-80, 1979.
    2. Bricker, T.M.: The structure and function of CPa-1 and CPa-2 in photosystem II.-Photosynth. Res. 24: 1-13, 1990. Go to original source...
    3. Groot, M.L., Peterman, E.J.G., van Stokkum, I.H.M., Dekker, J.P., van Grondelle, R.: Triplet and fluorescing states of the CP47 antenna complex of photosystem II studied as a function of temperature.-Biophys. J. 68: 281-290, 1995. Go to original source...
    4. Hemelrijk, P.W., Kwa, S.L.S., van Grondelle, R., Dekker, J.P.: Spectroscopic properties of LHC-II, the main light-harvesting chlorophyll a/b protein complex from chloroplast membranes.-Biochim. biophys. Acta 1098: 159-166, 1992. Go to original source...
    5. Knoetzel, J., Svendsen, I., Simpson, D.J.: Identification of the photosystem I antenna polypeptides in barley. Isolation of three pigment-binding antenna complexes.-Eur. J. Biochem. 206: 209-215, 1992. Go to original source...
    6. Murakami, S.: Structural and functional organization of the thylakoid membrane system in photosynthetic apparatus.-J. Electron Microsc. 41: 424-433, 1992.
    7. Mustárdy, L.: Development of thylakoid membrane stacking.-In: Ort, D.R., Yocum, C.F. (ed.): Oxygenic Photosynthesis: The Light Reactions. Pp. 59-68. Kluwer Academic Publ., Dordrecht-Boston-London 1996. Go to original source...
    8. Paolillo, D.J., Jr.: The three dimensional arrangement of internal lamellae in chloroplasts.-J. Cell Sci. 6: 243-255, 1970. Go to original source...
    9. Vácha, M., Yokoyama, H., Tokizaki, T., Furuki, M., Tani, T.: Laser scanning microscope for low temperature single molecule and microscale spectroscopy based on gradient index optics.-Rev. sci. Instrum. 70: 2041-2045, 1999. Go to original source...

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