Photosynthetica 2000, 38(4):505-512 | DOI: 10.1023/A:1012449104831

Analysis of Photosynthetic Activity at the Leaf and Canopy Levels from Reflectance Measurements: A Case Study

M. Méthy1
1 Dream Unit, Centre d'Ecologie Fonctionnelle et Evolutive, CNRS, Montpellier Cedex 5, France

The photochemical reflectance index (PRI), based on reflectance signatures at 531 and 570 nm, and associated with xanthophyll pigment inter-conversion and related thylakoid energisation, was evaluated as an indicator of photosynthetic function in a Mediterranean holm oak (Quercus ilex L.) coppice. The chlorophyll fluorescence pulse-amplitude-modulation and the eddy correlation techniques were used to estimate the photosystem 2 photochemical efficiency of leaves and the CO2 flux over the canopy, respectively. The reflectance and fluorescence techniques yielded identical estimates of the photosynthetic activity in leaves exposed to dark-light-dark cycles or to a variable irradiance in laboratory. However, there was no such correlation between photosynthetic performance and PRI when applied to a sun-exposed canopy in field conditions. Fluorescence profiles inside the canopy and especially a helpful use of multispectral reflectance imaging highlight the limitations of such method.

Additional key words: fluorescence; imaging; photochemical reflectance index; photosynthetic activity; Quercus ilex

Prepublished online: August 1, 2000; Published: November 1, 2000  Show citation

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Méthy, M. (2000). Analysis of Photosynthetic Activity at the Leaf and Canopy Levels from Reflectance Measurements: A Case Study. Photosynthetica, 38(4), 505-512. doi: 10.1023/A:1012449104831
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