Photosynthetica 2000, 38(4):513-519 | DOI: 10.1023/A:1012401221669

Analysis of Qualitative Contribution of Assimilatory and Non-Assimilatory De-Excitation Processes to Adaptation of Photosynthetic Apparatus of Barley Plants to High Irradiance

I. Kurasová1, M. Èajánek1, J. Kalina1, V. ©punda2
1 Faculty of Science, Department of Physics, Ostrava University, Ostrava, Czech Republic
2 Faculty of Science, Department of Physics, Ostrava University, Ostrava, Czech Republic

The adaptation of barley (Hordeum vulgare L. cv. Akcent) plants to low (LI, 50 µmol m-2 s-1) and high (HI, 1000 µmol m-2 s-1) growth irradiances was studied using the simultaneous measurements of the photosynthetic oxygen evolution and chlorophyll a (Chl a) fluorescence at room temperature. If measured under ambient CO2 concentration, neither increase of the oxygen evolution rate (P) nor enhancement of non-radiative dissipation of the absorbed excitation energy within photosystem 2 (PS2) (determined as non-photochemical quenching of Chl a fluorescence, NPQ) were observed for HI plants compared with LI plants. Nevertheless, the HI plants exhibited a significantly higher proportion of QA in oxidised state (estimated from photochemical quenching of Chl a fluorescence, qP), by 49-102 % at irradiances above 200 µmol m-2 s-1 and an about 1.5 fold increase of irradiance-saturated PS2 electron transport rate (ETR) as compared to LI plants. At high CO2 concentration the degree of P stimulation was approximately three times higher for HI than for LI plants, and the irradiance-saturated P values at irradiances of 2 440 and 2 900 µmol m-2 s-1 were by 130 and 150 % higher for HI plants than for LI plants. We suggest that non-assimilatory electron transport dominates in the adaptation of the photosynthetic apparatus of barley grown at high irradiances under ambient CO2 rather than an increased NPQ or an enhancement of irradiance-saturated photosynthesis.

Additional key words: chlorophyll fluorescence; Hordeum vulgare; non-radiative dissipation; non-assimilatory electron transports; photosynthetic oxygen evolution

Prepublished online: August 1, 2000; Published: November 1, 2000  Show citation

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Kurasová, I., Èajánek, M., Kalina, J., & ©punda, V. (2000). Analysis of Qualitative Contribution of Assimilatory and Non-Assimilatory De-Excitation Processes to Adaptation of Photosynthetic Apparatus of Barley Plants to High Irradiance. Photosynthetica, 38(4), 513-519. doi: 10.1023/A:1012401221669
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    References

    1. Asada, K.: The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons.-Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 601-639, 1999. Go to original source...
    2. Barber, J.: Molecular basis of the vulnerability of photosystem II to damage by light.-Aust. J. Plant Physiol. 22: 201-208, 1995. Go to original source...
    3. Biehler, K., Fock, H.: Evidence for the contribution of the Mehler-peroxidase reaction in dissipating excess electrons in drought-stressed wheat.-Plant Physiol. 112: 265-272, 1996. Go to original source...
    4. Bilger, W., Schreiber, U.: Energy-dependent quenching of dark-level chlorophyll fluorescence in intact leaves.-Photosynth. Res. 10: 303-308, 1986. Go to original source...
    5. Èajánek, M., Hudcová, M., Kalina, J., Lachetová, I., ©punda, V.: Gradual disassembly of photosystem 2 in vivo induced by excess irradiance. A hypothesis based on changes in 77 K fluorescence spectra of chlorophyll a in barley leaves.-Photosynthetica 37: 295-306, 1999. Go to original source...
    6. Chow, W.S.: Photoprotection and photoinhibitory damage.-Adv. mol. cell. Biol. 10: 151-196, 1994. Go to original source...
    7. Delieu, T.J., Walker, D.A.: Simultaneous measurement of oxygen evolution and chlorophyll fluorescence from leaf pieces.-Plant Physiol. 73: 534-541, 1983. Go to original source...
    8. Demmig-Adams, B.: Carotenoids and photoprotection in plants: A role for the xanthophyll zeaxanthin.-Biochim. biophys. Acta 1020: 1-24, 1990. Go to original source...
    9. Demmig-Adams, B., Adams, W.W., III.: The role of xanthophyll cycle carotenoids in the protection of photosynthesis.-Trends Plant Sci. 1: 21-26, 1996. Go to original source...
    10. Demmig-Adams, B., Adams, W.W., III, Czygan, F.-C., Schreiber, U., Lange, O.L.: Differences in the capacity for radiationless energy dissipation in the photochemical apparatus of green and blue-green algal lichens associated with differences in carotenoid composition.-Planta 180: 582-589, 1990. Go to original source...
    11. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    12. Genty, B., Harbinson, J.: Regulation of photosynthetic light utilization.-In: Baker, N.R. (ed.): Photosynthesis and the Environment. Pp. 67-99. Kluwer Academic Publ., Dordrecht 1997.
    13. Gilmore, A.M.: Mechanistic aspects of xanthophyll cycle-dependent photoprotection in higher plant chloroplasts and leaves.-Physiol. Plant. 99: 197-209, 1997. Go to original source...
    14. Gilmore, A.M., Yamamoto, H.Y.: Linear models relating xanthophylls and lumen acidity to non-photochemical fluorescence quenching. Evidence that antheraxanthin explains zeaxanthin-independent quenching.-Photosynth. Res. 35: 67-78, 1993. Go to original source...
    15. Gray, G.R., Savitch, L.V., Ivanov, A.G., Huner, N.P.A.: Photosystem II excitation pressure and development of resistance to photoinhibition. II. Adjustment of photosynthetic capacity in winter wheat and winter rye.-Plant Physiol. 110: 61-71, 1996. Go to original source...
    16. Heber, U., Bligny, R., Streb, P., Douce, R.: Photorespiration is essential for the protection of the photosynthetic apparatus of C3 plants against photoinactivation under sunlight.-Bot. Acta 109: 307-315, 1996. Go to original source...
    17. Horton, P., Ruban, A., Walters, R.G.: Regulation of light harvesting in green plants. Indication by nonphotochemical quenching of chlorophyll fluorescence.-Plant Physiol. 106: 415-420, 1994. Go to original source...
    18. Horton, P., Ruban, A.V., Walters, R.G.: Regulation of light harvesting in green plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 655-684, 1996. Go to original source...
    19. Huner, N.P.A., Öquist, G., Hurry, V.M., Krol, M., Falk, S., Griffith, M.: Photosynthesis, photoinhibition and low temperature acclimation in cold tolerant plants.-Photosynth. Res. 37: 19-39, 1993. Go to original source...
    20. Huner, N.P.A., Öquist, G., Sarhan, F.: Energy balance and acclimation to light and cold.-Trends Plant Sci. 3: 224-230, 1998. Go to original source...
    21. Ivanov, A.G., Morgan, R.M., Gray, G.R., Velitchkova, M.Y., Huner, N.P.A.: Temperature/light dependent development of selective resistance to photoinhibition of photosystem I.-FEBS Lett. 430: 288-292, 1998. Go to original source...
    22. Lapointe, L., Huner, N.P.A., Leblanc, R.M., Carpentier, R.: Possible photoacoustic detection of cyclic electron transport around Photosystem II in photoinhibited thylakoid preparations.-Biochim. biophys. Acta 1142: 43-48, 1993. Go to original source...
    23. Lichtenthaler, H.K.: Chlorophylls and carotenoids - pigments of photosynthetic biomembranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987. Go to original source...
    24. Lichtenthaler, H.K., Buschmann, C., Döll, M., Fietz, H.-J., Bach, T., Kozel, U., Meier, D., Rahmsdorf, U.: Photosynthetic activity, chloroplast ultrastructure, and leaf characteristics of high-light and low-light plants and of sun and shade leaves.-Photosynth. Res. 2: 115-141, 1981. Go to original source...
    25. Lichtenthaler, H.K., Kuhn, G., Prenzel, U., Buschmann, C., Meier, D.: Adaptation of chloroplast-ultrastructure and of chlorophyll-protein levels to high-light and low-light growth conditions.-Z. Naturforsch. 37c: 464-475, 1982. Go to original source...
    26. Logan, B.A., Barker, D.H., Demmig-Adams, B., Adams, W.W., III: Acclimation of leaf carotenoid composition and ascorbate levels to gradients in the light environment within an Australian rainforest.-Plant Cell Environ. 19: 1083-1090, 1996. Go to original source...
    27. Maxwell, D.P., Falk, S., Huner, N.P.A.: Photosystem II excitation pressure and development of resistance to photoinhibition. I. Light-harvesting complex II abundance and zeaxanthin content in Chlorella vulgaris.-Plant Physiol. 107: 687-694, 1995. Go to original source...
    28. Melis, A.: Photostasis in plants. Mechanisms and regulation.-In: Williams, T.P., Thistle, A.B. (ed.): Photostasis and Related Phenomena. Pp. 207-221. Plenum Press, New York 1998. Go to original source...
    29. Melis, A.: Photosystem-II damage and repair cycle in chloroplasts: what modulates the rate of photodamage in vivo?-Trends Plant Sci. 4: 130-135, 1999. Go to original source...
    30. Niyogi, K.K.: Photoprotection revised: Genetic and molecular approaches.-Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 333-359, 1999. Go to original source...
    31. Osmond, C.B., Grace, S.C.: Perspectives on photoinhibition and photorespiration in the field: quintessential inefficiencies of the light and dark reactions of photosynthesis?-J. exp. Bot. 46: 1351-1362, 1995. Go to original source...
    32. Park, Y.-I., Chow, W.S., Anderson, J.M.: Antenna size dependency of photoinactivation of photosystem II in light-acclimated pea leaves.-Plant Physiol. 115: 151-157, 1997. Go to original source...
    33. Park, Y.-I., Chow, W.S., Osmond, C.B., Anderson, J.M.: Electron transport to oxygen mitigates against the photoinactivation of Photosystem II in vivo.-Photosynth. Res. 50: 23-32, 1996. Go to original source...
    34. Polle, A.: Mehler reaction: Friend or foe in photosynthesis?-Bot. Acta 109: 84-89, 1996. Go to original source...
    35. Schreiber, U., Neubauer, C.: O2-dependent electron flow, membrane energization and the mechanism of non-photochemical quenching of chlorophyll fluorescence.-Photosynth. Res. 25: 279-293, 1990. Go to original source...
    36. Sivak, M.N., Walker, D.A.: Chlorophyll a fluorescence: can it shed light on fundamental questions in photosynthetic carbon dioxide fixation?-Plant Cell Environ. 8: 439-448, 1985. Go to original source...
    37. ©punda, V., Èajánek, M., Kalina, J., Lachetová, I., ©prtová, M., Marek, M.V.: Mechanistic differences in utilization of absorbed excitation energy within photosynthetic apparatus of Norway spruce induced by the vertical distribution of photosynthetically active radiation through the tree crown.-Plant Sci. 133: 155-165, 1998. Go to original source...
    38. Wu, J., Neimanis, S., Heber, U.: Photorespiration is more effective than the Mehler reaction in protecting the photosynthetic apparatus against photoinhibition.-Bot. Acta 104: 283-291, 1991. Go to original source...

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