Photosynthetica 2000, 38(4):539-551 | DOI: 10.1023/A:1012409423487

Multicolour Fluorescence Imaging of Sugar Beet Leaves with Different Nitrogen Status by Flash Lamp UV-Excitation

G. Langsdorf1, C. Buschmann2, M. Sowinska3, F. Babani2, M. Mokry3, F. Timmermann1, H.K. Lichtenthaler2
1 Botanical Institute II, University of Karlsruhe, Karlsruhe, Germany
2 Botanical Institute II, University of Karlsruhe, Karlsruhe, Germany
3 Groupe d'Optique Appliquée (GOA), CNRS, 23, Strasbourg Cedex 2, France

Fluorescence images of leaves of sugar beet plants (Beta vulgaris L. cv. Patricia) grown on an experimental field with different fertilisation doses of nitrogen [0, 3, 6, 9, 12, 15 g(N) m-2] were taken, applying a new multicolour flash-lamp fluorescence imaging system (FL-FIS). Fluorescence was excited by the UV-range (280-400 nm, λmax = 340 nm) of a pulsed Xenon lamp. The images were acquired successively in the four fluorescence bands of leaves near 440, 520, 690, and 740 nm (F440, F520, F690, F740) by means of a CCD-camera. Parallel measurements were performed to characterise the physiological state of the leaves (nitrogen content, invert-sugars, chlorophylls and carotenoids as well as chlorophyll fluorescence induction kinetics and beet yield). The fluorescence images indicated a differential local patchiness across the leaf blade for the four fluorescence bands. The blue (F440) and green fluorescence (F520) were high in the leaf veins, whereas the red (F690) and far-red (F740) chlorophyll (Chl) fluorescences were more pronounced in the intercostal leaf areas. Sugar beet plants with high N supply could be distinguished from beet plants with low N supply by lower values of F440/F690 and F440/F740. Both the blue-green fluorescence and the Chl fluorescence rose at a higher N application. This increase was more pronounced for the Chl fluorescence than for the blue-green one. The results demonstrate that fluorescence ratio imaging of leaves can be applied for a non-destructive monitoring of differences in nitrogen supply. The FL-FIS is a valuable diagnostic tool for screening site-specific differences in N-availability which is required for precision farming.

Additional key words: Beta vulgaris L.; blue-green fluorescence; chlorophyll fluorescence; fluorescence ratios; nitrogen nutrition; photosynthetic activity; protein; sugars; yield

Prepublished online: August 1, 2000; Published: November 1, 2000  Show citation

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Langsdorf, G., Buschmann, C., Sowinska, M., Babani, F., Mokry, M., Timmermann, F., & Lichtenthaler, H.K. (2000). Multicolour Fluorescence Imaging of Sugar Beet Leaves with Different Nitrogen Status by Flash Lamp UV-Excitation. Photosynthetica, 38(4), 539-551. doi: 10.1023/A:1012409423487
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    References

    1. Araus, J.L., Amaro, T., Voltas, J., Nakkoul, H., Nachit, M.M.: Chlorophyll fluorescence as a selection criterion for grain yield in durum wheat under Mediterranean conditions.-Field Crops Res. 55: 209-223, 1998. Go to original source...
    2. Babani, F., Lichtenthaler, H.K.: Light-induced and age-dependent development of chloroplasts in etiolated barley leaves as visualized by determination of photosynthetic pigments, CO2 assimilation rates and different kinds of chlorophyll fluorescence ratios.-J. Plant Physiol. 148: 555-566, 1996. Go to original source...
    3. Baffler, R.: Handbuch der landwirtschaftlichen Versuchs-und Untersuchungsmethodik. Futtermitteluntersuchung. Vol. III.-Pp. 1-4. VDLUFA, Darmstadt 1997a.
    4. Baffler, R.: Handbuch der landwirtschaftlichen Versuchs-und Untersuchungsmethodik. Umweltanalytik. Vol. VII.-Pp. 1-6. VDLUFA, Darmstadt 1997b.
    5. Barósi, A., Kocsányi, L., Várkonyi, S., Richter, P., Csintalan, Z., Szente, K.: Two-wavelength, multipurpose, truly portable chlorophyll fluorometer and its application in field monitoring of phytomediation.-Meas. Sci. Technol. 11: 717-729, 2000. Go to original source...
    6. Bergmann, W.: Nutritional Disorders of Plants: Development, Visual and Analytical Diagnosis.-G. Fischer, Jena 1992.
    7. Bornscheuer, E.: Zucker-und Futterrüben (Beta vulgaris L.).-In: Oehmichen, J. (ed.): Pflanzenproduktion. Vol. II: Produktionstechnik.-Pp. 385-422. Paul Parey, Berlin 1986.
    8. Bullock, D.G., Anderson, D.S.: Evaluation of the Minolta SPAD-502 chlorophyll meter for nitrogen management in corn.-J. Plant Nutr. 21: 741-755, 1998. Go to original source...
    9. Buschmann, C.: The characterization of the developing photosynthetic apparatus in greening barley leaves by means of (slow) fluorescence kinetic measurements.-In: Akoyunoglou, G. (ed.): Photosynthesis. Vol. V. Pp. 417-426. Balaban International Science Services, Philadelphia 1981.
    10. Buschmann, C., Langsdorf, G., Lichtenthaler, H.K.: Imaging of the blue, green and red fluorescence emission of plants: an overview.-Photosynthetica 38: 483-492, 2000. Go to original source...
    11. Buschmann, C., Lichtenthaler, H.K.: Principles and characteristics of multi-colour fluorescence imaging of plants.-J. Plant Physiol. 152: 297-314, 1998. Go to original source...
    12. Cechin, I.: Photosynthesis and chlorophyll fluorescence in two hybrids of sorghum under different nitrogen and water regimes.-Photosynthetica 35: 233-240, 1998. Go to original source...
    13. Cerovic, Z.G., Samson, G., Morales, F., Trembley, N., Moya, I.: Ultraviolet-induced fluorescence for plant monitoring: present state and prospects.-Agronomy 19: 543-578, 1999. Go to original source...
    14. Ciompi, S., Gentili, E., Guidi, L., Soldatini, G.F.: The effect of nitrogen deficiency on leaf gas exchange and chlorophyll fluorescence parameters in sunflower.-Plant Sci. 118: 117-184, 1996. Go to original source...
    15. Evans, J.R.: Photosynthesis and nitrogen relationships in leaves of C3 plants.-Oecologia 78: 9-19, 1989. Go to original source...
    16. Filella, I., Serrano, L., Serra, J., Penuelas, J.: Evaluating wheat nitrogen status with canopy reflectance indices and discriminant analysis.-Crop Sci. 35: 1400-1405, 1995. Go to original source...
    17. Fracheboud, Y., Haldimann, P., Leipner, J., Stamp, P.: Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize (Zea mays L.).-J. exp. Bot. 50: 1533-1540, 1999. Go to original source...
    18. Georgieva, K., Lichtenthaler, H.K.: Photosynthetic activity and acclimation ability of pea plants to low and high temperature treatment as studied by means of chlorophyll fluorescence.-J. Plant Physiol. 155: 416-423, 1999. Go to original source...
    19. Gitelson, A.A., Buschmann, C., Lichtenthaler, H.K.: The chlorophyll fluorescence ratio F735/F700 as an accurate measure of the chlorophyll content in plants.-Remote Sens. Envir. 69: 296-302, 1999. Go to original source...
    20. Hák, R., Rinderle-Zimmer, U., Lichtenthaler, H.K., Nátr, L.: Chlorophyll a fluorescence signatures of nitrogen deficient barley leaves.-Photosynthetica 28: 151-159, 1993.
    21. Heisel, F., Sowinska, M., Eckert, C., Miehé, J.A.: Detection of vegetation stress and nutrient deficiencies by leaf laser-induced fluorescence imaging.-Acta Hort. 496: 245-252, 1999. Go to original source...
    22. Heisel, F., Sowinska, M., Khalili, E., Eckert, C., Miehé, J.A., Lichtenthaler, H.K.: Laser-induced fluorescence imaging for monitoring nitrogen fertilising treatments of wheat.-SPIE 3059: 10-21, 1997. Go to original source...
    23. Heisel, F., Sowinska, M., Miehé, J.A., Lang, M., Lichtenthaler, H.K.: Detection of nutrient deficiencies of maize by laser induced fluorescence imaging.-J. Plant Physiol. 148: 622-631, 1996. Go to original source...
    24. Jalinek, H., van der Schoor, R., Frandas, A., van Pijlen, J.G., Bino, R.J.: Chlorophyll fluorescence of Brassica oleracea seeds as a non-destructive marker for seed maturity and seed performance.-Seed Sci. Res. 8: 437-443, 1998. Go to original source...
    25. Johnson, G.A., Mantha, S.V., Day, T.A.: A spectrofluorometric survey of UV-induced blue-green fluorescence in foliage of 35 species.-J. Plant Physiol. 156: 242-252, 2000. Go to original source...
    26. Khamis, S., Lamaze, T., Lemoine, Y., Foyer, C.: Adaptation of the photosynthetic apparatus in maize leaves as a result of nitrogen limitation. Relationships between electron transport and carbon assimilation.-Plant Physiol. 94: 1436-1443, 1990. Go to original source...
    27. Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: The basics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313-349, 1991. Go to original source...
    28. Lang, M., Lichtenthaler, H.K., Sowinska, M., Heisel, F., Miehé, J.A.: Fluorescence imaging of water and temperature stress in plant leaves.-J. Plant Physiol. 148: 613-621, 1996. Go to original source...
    29. Lang, M., Lichtenthaler, H.K., Sowinska, M., Summ, P., Heisel, F.: Blue, green, and red fluorescence signatures and images of tobacco leaves.-Bot. Acta 107: 230-236, 1994. Go to original source...
    30. Lang, M., Stober, F., Lichtenthaler, H.K.: Fluorescence emission spectra of plant leaves and constituents.-Radiat. environ. Biophys. 30: 333-347, 1991. Go to original source...
    31. Lichtenthaler, H.K.: Chlorophylls and carotenoids-pigments of photosynthetic biomembranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987. Go to original source...
    32. Lichtenthaler, H.K., Babani, F., Langsdorf, G., Buschmann, C.: Measurement of differences in red chlorophyll fluorescence and photosynthetic activity between sun and shade leaves by fluorescence imaging.-Photosynthetica 38: 523-531, 2000. Go to original source...
    33. Lichtenthaler, H.K., Buschmann, C., Döll, M., Fietz, H.-J., Bach, T., Kozel, U., Meier, D., Rahmsdorf, U.: Photosynthetic activity, chloroplast ultrastructure, and leaf characteristics of high-light and low-light plants and of sun and shade leaves.-Photosynth. Res. 2: 115-141, 1981. Go to original source...
    34. Lichtenthaler, H.K., Kuhn, G., Prenzel, U., Buschmann, C., Meier, D.: Adaptation of chloroplast-ultrastructure and of chlorophyll-protein levels to high-light and low-light growth conditions.-Z. Naturforsch. 37c: 464-475, 1982. Go to original source...
    35. Lichtenthaler, H.K., Lang, M., Sowinska, M., Heisel, F., Miehé, J.A.: Detection of vegetation stress via a new high resolution fluorescence imaging system.-J. Plant Physiol. 148: 599-612, 1996. Go to original source...
    36. Lichtenthaler, H.K., Miehé, J.A.: Fluorescence imaging as a diagnostic tool for plant stress.-Trends Plant Sci. 2: 316-320, 1997. Go to original source...
    37. Lichtenthaler, H.K., Rinderle, U.: The role of chlorophyll fluorescence in the detection of stress conditions in plants.-CRC crit. Rev. anal. Chem. 19(Suppl. 1): S29-S85, 1988. Go to original source...
    38. Lichtenthaler, H.K., Schweiger, J.: Cell wall bound ferulic acid, the major substance of the blue-green fluorescence emission of plants.-J. Plant Physiol. 152: 272-282, 1998. Go to original source...
    39. Lima, J.D., Mosquim, P.R., da Matta, F.M.: Leaf gas exchange and chlorophyll fluorescence parameters in Phaseolus vulgaris as affected by nitrogen and phosphorus deficiency.-Photosynthetica 37: 113-121, 1999. Go to original source...
    40. Ma, B.L., Morrison, M.J., Dwyer, L.M.: Canopy light reflectance and field greenness to assess nitrogen fertilization and yield of maize.-Agron. J. 88: 915-920, 1996. Go to original source...
    41. Markwell, J., Osterman, J.C., Mitchell, J.L.: Calibration of the Minolta SPAD-502 leaf chlorophyll meter.-Photosynth. Res. 46: 467-472, 1995. Go to original source...
    42. Marschner, H.: Mineral Nutrition of Higher Plants.-Academic Press, London 1995.
    43. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence-a practical guide.-J. exp. Bot. 51: 659-668, 2000. Go to original source...
    44. Moraghan, J.T.: Sugarbeet canopy type and accumulation of plant nitrogen as delineated by aerial photography and global positioning systems.-Commun. Soil Sci. Plant Anal. 29: 2953-2959, 1998. Go to original source...
    45. Morales, F., Cerovic, Z.G., Moya, I.: Time-resolved blue-green fluorescence of sugar beet (Beta vulgaris L.) leaves. Spectroscopic evidence for the presence of ferulic acid as the main fluorophore of the epidermis.-Biochim. biophys. Acta 1273: 251-262, 1996. Go to original source...
    46. Reusch, S.: Entwicklung eines reflexionsoptischen Sensors zur Erfassung der Stickstoffversorgung landwirtschaftlicher Kulturpflanzen.-PhD-Thesis, Kiel 1997.
    47. Richter, M., Goss, R., Wagner, B., Holzwarth, A.R.: Characterization of the fast and slow reversible components of non-photochemical quenching in isolated pea thylakoids by picosecond time-resolved chlorophyll fluorescence.-Biochemistry 38: 12718-12726, 1999. Go to original source...
    48. Rosema, A., Snel, J.F.H., Zahn, H., Buurmeijer, W.F., Van Hove, L.W.A.: The relation between laser-induced chlorophyll fluorescence and photosynthesis.-Remote Sens. Environ. 65: 143-154, 1998. Go to original source...
    49. Röver, A.: Beschreibung wichtiger Streßsituationen während des vegetativen Wachstums der Zuckerrübe.-Zuckerindustrie 123: 683-687, 1998.
    50. Samson, G., Prá¹il, O., Yaakoubd, B.: Photochemical and thermal phases of chlorophyll a fluorescence.-Photosynthetica 37: 163-182, 1999. Go to original source...
    51. Schepers, J.S., Blackmer, T.M., Francis, D.D.: Chlorophyll meter method for estimating nitrogen content in plant tissue.-In: Kalra, Y.P. (ed.): Handbook of Reference Methods for Plant Analysis. Pp. 129-135. CRC Press, Boca Raton 1998. Go to original source...
    52. Schweiger, J.: Untersuchungen über die möglichen Beziehungen zwischen Blaugrünfluoreszenz und dem Phenolgehalt der Pflanzen.-Karlsruhe Contrib. Plant Physiol. 34: 1-132, 1999.
    53. Schweiger, J., Lang, M., Lichtenthaler, H.K.: Differences in excitation spectra of leaves between stressed and non-stressed plants.-J. Plant Physiol. 148: 536-547, 1996. Go to original source...
    54. ©esták, Z., ©iffel, P.: Leaf-age related differences in chlorophyll fluorescence.-Photosynthetica 33: 347-369, 1997.
    55. Shangguan, Z., Shao, M., Dyckmans, J.: Effects of nitrogen nutrition and water deficit on net photosynthetic rate and chlorophyll fluorescence in winter wheat.-J. Plant Physiol. 156: 46-51, 2000. Go to original source...
    56. Sowinska, M., Cunin, B., Deruyver, A., Heisel, F., Miehé, J.A., Langsdorf, G., Lichtenthaler, H.K.: Near-field measurements of vegetation by laser-induced fluorescence imaging.-SPIE 3868: 120-131, 1999. Go to original source...
    57. Sowinska, M., Deckers, T., Eckert, C., Heisel, F., Valcke, R., Miehé, J.A.: Evaluation of nitrogen fertilization effect on apple-tree leaves and fruit by fluorescence imaging.-SPIE 3382: 100-110, 1998. Go to original source...
    58. Stober, F., Lichtenthaler, H.K.: Characterization of the laser-induced blue, green and red fluorescence signatures of leaves of wheat and soybean grown under different irradiance.-Physiol. Plant. 88: 696-704, 1993. Go to original source...
    59. Strasser, R.J., Srivastava, A., Govindjee: Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria.-Photochem. Photobiol. 61: 32-42, 1995. Go to original source...
    60. Strasser, R.J., Tsimilli-Michael, M.: The JIP-test as a tool for the detection and quantification of stress in sustainable agriculture.-In: El Bassam, N., Behl, R.K., Prochnow, B. (ed.): Sustainable Agriculture for Food, Energy and Industry. Pp. 133-139. James &; James, London 1998.
    61. Subhash, N., Wenzel, O., Lichtenthaler, H.K.: Changes in blue-green and chlorophyll fluorescence emission and fluorescence ratios during senescence of tobacco plants.-Remote Sens. Environ. 69: 215-223, 1999. Go to original source...
    62. Sylvester-Bradley, R., Lord, E., Sparkes, D.L., Scott, R.K., Wiltshire, J.J.J., Orson, J.: An analysis of the potential of precision farming in Northern Europe.-Soil Use Management 15: 1-8, 1999. Go to original source...
    63. Tyystjärvi, E., Koski, A., Keränen, M., Nevalainen, O.: The Kautsky curve is a built-in barcode.-Biophys. J. 77: 1159-1167, 1999. Go to original source...
    64. Van Gorkom, H.: Fluorescence measurements in the study of photosystem II electron transport.-In: Govindjee, Amesz, J., Fork, D.C. (ed.): Light Emission by Plants and Bacteria. Pp. 267-289. Academic Press, Orlando-San Diego-New York-Austin-Boston-London-Sydney-Toyo-Toronto 1986. Go to original source...
    65. Vouillot, M.O., Huet, P., Boissard, P.: Early detection of N deficiency in a wheat crop using physiological and radiometric methods.-Agronomy 18: 117-130, 1998. Go to original source...

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