Photosynthetica 2001, 39(1):17-22 | DOI: 10.1023/A:1012427415388

Photoinhibition in Chilling Stressed Leguminosae: Comparison of Vicia Faba and Pisum Sativum

F.C. Lidon1, G. Ribeiro1, H. Santana1, H. Marques1, K. Correia1, S. Gouveia1
1 Faculdade Ciências Tecnologia, Universidade Nova Lisboa, Monte Cafarica, Portugal

The concentrations of photosynthetic pigments decreased in both chilling stressed species but the ratios of chlorophyll (Chl) a/b and total carotenoids (Car)/Chls were depressed only in faba bean. The contents of α+β carotene and lutein+lutein-5,6-epoxide remained unaffected in both species, but the de-epoxidation state involving the components of xanthophyll cycle increased in pea. Under chilling stress the photosynthetic electron transport associated with photosystem 2, PS2 (with and without the water oxidising complex) decreased in both plant species, the inhibition being higher in faba bean. The intrachloroplast quinone pool also decreased in both stressed species, yet an opposite trend was found for cytochrome b559LP. Under stress an increasing peroxidation of thylakoid acyl lipids was detected in pea, but higher protein/Chl ratio was detected in faba bean. Thus the acceptor side of PS2 is mostly affected in both chilling stressed species, but faba bean is more sensitive.

Additional key words: carotenoids; chlorophylls; cytochromes; lipids; photoinhibition; photosystem 2; proteins; quinones; water oxidising complex; xanthophyll cycle

Published: March 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Lidon, F.C., Ribeiro, G., Santana, H., Marques, H., Correia, K., & Gouveia, S. (2001). Photoinhibition in Chilling Stressed Leguminosae: Comparison of Vicia Faba and Pisum Sativum. Photosynthetica, 39(1), 17-22. doi: 10.1023/A:1012427415388
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Barber, J., Andersson, B.: Too much of a good thing: light can be bad for photosynthesis.-Trends biochem. Sci. 17: 61-66, 1992. Go to original source...
    2. Bilger, W., Björkman, O.: Temperature dependence of violaxanthin de-epoxidation and non-photochemical fluorescence quenching in intact leaves of Gossypium hirsutum L. and Malva parviflora L.-Planta 184: 226-234, 1991. Go to original source...
    3. Buser, C.A., Diner, B.A., Brudvig, G.W.: Photooxidation of cytochrome b 559 in oxygen evolving photosystem II.-Biochemistry 31: 11449-11459, 1992. Go to original source...
    4. Buser, C.A, Thompson, L.K., Diner, B.A., Brudvig, G.W.: Electron-transfer reactions in manganese-depleted photosystem II.-Biochemistry 29: 8977-8985, 1990. Go to original source...
    5. Cakmak, I., Horst, W.J.: Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean.-Physiol. Plant. 83: 463-468, 1991. Go to original source...
    6. Canaani, O., Havaux, M.: Evidence for a biological role in photosynthesis for cytochrome b 559 - a component of photosystem II reaction center.-Proc. nat. Acad. Sci. USA 87: 9295-9299, 1990. Go to original source...
    7. Cogdell, R.J., Frank, H.A.: How carotenoids function in photosynthetic bacteria.-Biochim. biophys. Acta 895: 63-79, 1987. Go to original source...
    8. De Las Rivas, J., Telfer, A., Barber, J.: Two coupled β-carotene molecules protect P680 from photodamage in isolated Photosystem II reaction centres.-Biochim. biophys. Acta 1142: 155-164, 1993. Go to original source...
    9. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599-626, 1992. Go to original source...
    10. Demmig-Adams, B., Winter, K., Krüger, A., Czygan, F.-C.: Zeaxanthin synthesis, energy dissipation, and photoprotection of photosystem II at chilling temperatures.-Plant Physiol. 90: 894-898, 1989. Go to original source...
    11. Droppa, M., Masojidek, J., Rózsa, Z., Wolak, A., Horváth, L.I., Farkas, T., Horváth, G.: Characteristics of Cu deficiency-induced inhibition of photosynthetic electron transport in spinach chloroplasts.-Biochim. biophys. Acta 891: 75-84, 1987. Go to original source...
    12. Erixon, K., Butler, W.L.: The relationship between Q, C-550 and cytochrome b 559 in photoreactions at -196° in chloroplasts.-Biochim. biophys. Acta 234: 381-389, 1971. Go to original source...
    13. Gong, H., Nilsen, S., Allen, J.F.: Photoinhibition of photosynthesis in vivo: involvement of multiple sites in a photodamage process under CO2 and O2-free conditions.-Biochim. biophys. Acta 1142: 115-122, 1993. Go to original source...
    14. Houchins, J.P., Hind, G.: Concentration and function of membrane-bound cytochromes in cyanobacterial heterocysts.-Plant Physiol. 76: 456-460, 1984. Go to original source...
    15. Krause, G.H.: Photoinhibition induced by low temperatures.-In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis from Molecular Mechanisms to the Field. Pp. 331-348. Bios Scientific Publ., Oxford 1994.
    16. Lichtenthaler, H.K.: Chlorophylls and carotenoids - pigments of photosynthetic biomembranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987. Go to original source...
    17. Lidon, F.C., Henriques, F.S.: Effects of copper on the nitrate to ammonia reduction mechanism in rice plants.-Photosynthetica 26: 371-380, 1992a.
    18. Lidon, F.C., Henriques, F.S.: Effects of excess copper on the photosynthetic pigments in rice plants.-Bot. Bull. Acad. sin. 33: 141-149, 1992b.
    19. Lidon, F.C., Henriques, F.S.: Changes in the contents ofphotosynthetic electron carriers, RNAse activity and membrane permeability triggered by excess copper in rice.-Photosynthetica 28: 99-108, 1993a.
    20. Lidon, F.C., Henriques, F.S.: Oxygen metabolism in higher plant chloroplasts.-Photosynthetica 29: 249-279, 1993b.
    21. Lidon, F.C., Teixeira, M.G.: Oxy radicals production and control in the chloroplasts of Mn-treated rice.-Plant Sci. 152: 7-15, 2000. Go to original source...
    22. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
    23. Owens, T.G., Shreve, A.P., Albrecht, A.C.: Dynamics and mechanism of singlet energy transfer between carotenoids and chlorophylls: Light-harvesting and non-photochemical fluorescence quenching.-In: Murata, N. (ed.): Research in Photosynthesis. Vol. IV. Pp. 179-186. Kluwer Academic Publ., Dordrecht-Boston-London 1992.
    24. Ramalho, J.C., Pons, T.L., Groeneveld, H.W., Nunes, M.A.: Photosynthetic responses of Coffea arabica leaves to a short-term high light exposure in relation to N availability.-Physiol. Plant. 101: 229-239, 1997. Go to original source...
    25. Redfearn, E.R., Friend, J.: Studies on plastoquinone. 1. Determination of the concentration and oxidation-reduction state of plastoquinone in isolated chloroplasts.-Phytochemistry 1: 147-151, 1962. Go to original source...
    26. Rees. D., Horton, P.: The mechanism of changes in photosystem II efficiency in spinach thylakoids.-Biochim. biophys. Acta 1016: 219-227, 1990. Go to original source...
    27. Schindler, C., Reith, P., Lichtenthaler, H.K.: Diferential levels of carotenoids and decrease of zeaxanthin cycle performance during leaf development in a green and an aurea variety of tobacco.-J. Plant Physiol. 143: 500-507, 1994. Go to original source...
    28. Siefermann-Harms, D.: Carotenoids in photosynthesis. I. Location in photosynthetic membranes and light-harvesting function.-Biochim. biophys. Acta 811: 325-355, 1985. Go to original source...
    29. Styring, S., Virgin, I., Ehrenberg, A., Andersson, B.: Strong light photoinhibition of electrontransport in Photosystem II. Impairment of the function of the first quinone acceptor, QA.-Biochim. biophys. Acta 1015: 269-278, 1990. Go to original source...
    30. Telfer, A., De Las Rivas, J., Barber, J.: β-carotene within the isolated Photosystem II reaction centre: photooxidation and irreversible bleaching of this chromophore by oxidised P680.-Biochim. biophys. Acta 1060: 106-114, 1991. Go to original source...
    31. Thayer, S.S., Björkman, O.: Leaf xanthophyll content and composition in sun and shade determined by HPLC.-Photosynth. Res. 23: 331-343, 1990. Go to original source...
    32. Vass, I., Mohanty, N., Demeter, S.: Photoinhibition of electron transport activity of photosystem II in isolated thylakoids studied by thermoluminescence and delayed luminescence.-Z. Naturforsch. 43c: 871-876, 1988. Go to original source...
    33. Vass, I., Styring, S., Hundal, T., Koivuniemi, A., Aro, E.-M., Andersson, B.: Reversible and irreversible intermediates during photoinhibition of photosystem II: Stable reduced QA species promote chlorophyll triplet formation.-Proc. nat. Acad. Sci. USA 89: 1408-1412, 1992. Go to original source...

    Return to the content