Photosynthetica 2001, 39(2):227-232 | DOI: 10.1023/A:1013787327147

Regulation Mechanism of Excitation Energy Transfer in Phycobilisome-Thylakoid Membrane Complexes

Ye Li1, Jianping Zhang1, Jingquan Zhao1, Lijing Jiang1
1 Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P.R. China

Regulation mechanism of excitation energy transfer between phycobilisomes (PBS) and the photosynthetic reaction centres was studied by the state transition techniques in PBS-thylakoid membrane complexes. DCMU, betaine, and N-ethylmaleimide were applied to search for the details of energy transfer properties based on the steady fluorescence measurement and individual deconvolution spectra at state 2 or state 1. The closure of photosystem (PS) 2 did not influence on fluorescence yields of PS1, i.e., energy could not spill to PS1 from PS2. When the energy transfer pathway from PBS to PS1 was disturbed, the relative fluorescence yield of PS2 was almost the same as that of PS2 in complexes without treatment. If PBSs were fixed by betaine, the state transition process was restrained. Hence PBS may detach from PS2 and become associated to PS1 at state 2. Our results contradict the proposed "spill-over" or "PBS detachment" models and support the mobile "PBS model".

Additional key words: absoprtion spectra; allophycocyanin; betaine; DCMU; fluorescence spectra; models of energy transfer; N-ethylmaleimide; photosystems 1 and 2; state transition

Published: June 1, 2001  Show citation

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Li, Y., Zhang, J., Zhao, J., & Jiang, L. (2001). Regulation Mechanism of Excitation Energy Transfer in Phycobilisome-Thylakoid Membrane Complexes. Photosynthetica, 39(2), 227-232. doi: 10.1023/A:1013787327147
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