Photosynthetica 2001, 39(4):521-527 | DOI: 10.1023/A:1015695727015

Development of Chloroplast in Dark-Grown Wheat Seedlings Irradiated at Elevated Temperature

A.K. Singh, G.S. Singhal

Elevated temperature inhibited the accumulation of chlorophyll and photosynthetic proteins, and the development of photochemical activity, however, carotenoids continued to accumulate. Signal transduction pathway involved in protochlorophyllide oxidoreductase was unaffected by elevated temperature of 38°C. Two-dimensional gel electrophoresis of stroma proteins showed similar patterns in the dark-grown seedlings and seedlings irradiated at elevated temperature, although some low molecular mass proteins accumulated at 38°C. In contrast, seedlings irradiated at 25°C showed complex pattern of proteins. Hence the development of chloroplast and its associated functions during irradiation of etiolated seedlings are inhibited by elevated temperature.

Additional key words: carotenoids; chlorophyll; nucleic acids; photosystem 2; proteins; Triticum aestivum

Published: December 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Singh, A.K., & Singhal, G.S. (2001). Development of Chloroplast in Dark-Grown Wheat Seedlings Irradiated at Elevated Temperature. Photosynthetica, 39(4), 521-527. doi: 10.1023/A:1015695727015
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Baumgartner, B.J., Rapp, J.C., Mullet, J.E.: Plastid transcription activity and DNA copy number increase early in barley chloroplast development.-Plant Physiol. 89: 1011-1018, 1989. Go to original source...
    3. Berry. J., Björkman, O.: Photosynthetic response and adaptation to temperature in higher plants.-Annu. Rev. Plant Physiol. 31: 491-543, 1980. Go to original source...
    4. Choniczynski, P., Sacchi, N.: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.-Anal. Biochem. 162: 156-159, 1987. Go to original source...
    5. Danon, A., Mayfield, S.P.Y.: Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins.-EMBO J. 10: 3993-4001, 1991. Go to original source...
    6. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599-626, 1992. Go to original source...
    7. Feierabend, J., Berberich, T.: Heat-induced ribosome-deficiency of plastids - mechanisms and applications.-In Mache, R., Stutz, E., Subramanian, A.R. (ed.): The Translational Apparatus of Photosynthetic Organelles. Pp. 215-227. Springer-Verlag, Berlin-Heidelberg-New York-London-Paris-Tokyo-Hong Kong-Barcelona-Budapest 1991. Go to original source...
    8. Gruissem, W.: Chloroplast gene expression: how plants turn their plastids on.-Cell 56: 161-170, 1989. Go to original source...
    9. Hochstrasser, D.F., Harrington, M.G., Hochstrasser, A.-C., Miler, M.J., Merryl, C.R.: Methods for increasing the resolution of two-dimensional protein electrophoresis.-Anal. Biochem. 173: 424-435, 1988. Go to original source...
    10. Kyle, D.J., Zalik, S.: Development of photochemical activity in relation to pigment and membrane protein accumulation in chloroplasts of barley and its virescent mutant.-Plant Physiol. 69: 1392-1400, 1982. Go to original source...
    11. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    12. Mayfield, S.P., Yohn, C.B., Cohen, A., Danon, A.: Regulation of chloroplast gene expression.-Annu. Rev. Plant Physiol. Plant mol. Biol. 46: 147-166, 1995. Go to original source...
    13. Mishra, N.P., Francke, C., van Gorkom, H.J., Ghanotakis, D.F.: Destructive role of singlet oxygen during aerobic illumination of the photosysterri II core complex.-Biochim. biophys. Acta 1186: 81-90, 1994. Go to original source...
    14. Mullet, I.E.: Chloroplast development and gene expression.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 475-502, 1988. Go to original source...
    15. Mullet, J.E., Klein, P.O., Klein, R.R.: Chlorophyll regulates accumulation of the plastid encoded chlorophyll apoproteins CP43 and D1 by increasing apoprotein stability.-Proc. nat. Acad. Sci. USA 87: 4038-4042, 1990. Go to original source...
    16. Reinbothe, S., Reinbothe, C.: Regulation of chlorophyll biosynthesis in angiosperms.-Plant Physiol. 111: 1-7, 1996. Go to original source...
    17. Reiter, R.S., Coomber, S.A., Bourett, T.M., Bartley, G.E., Scolnik, P.A.: Control of leaf and chloroplast development by the Arabidopsis gene pale cress.-Plant Cell 6: 1253-1264, 1994. Go to original source...
    18. Rochaix, J.D.: Post-transcription steps in expression of chloroplast genes.-Annu. Rev. Cell Biol. 8: 1-28, 1992. Go to original source...
    19. Sambrook. J., Fritsch, E.F., Maniatis, T.: Molecular Cloning. A Laboratory Manual. 2nd Ed.-Cold Spring Harbor Laboratory, Cold Spring Harbor 1989.
    20. Singh. A.K.. Chauhan, S., Singhal, G.S.: A simple and rapid aqueous method for the purification of DNA from wheat chloroplast.-J. Plant Biochem. Biotech. 4: 51-53, 1995. Go to original source...
    21. Singh, A.K., Singhal, G.S.: Formation of cross-linking between photosystem 2 proteins during irradiation of thylakoid membranes at high temperature.-Photosynthetica 36: 213-223, 1999. Go to original source...
    22. Tewari, A.K., Tripathy, B.C.: Temperature-stress-induced impairment of chlorophyll biosynthetic reactions in cucumber and wheat.-Plant Physiol. 117: 851-858, 1998. Go to original source...
    23. Thomas, E.J., Ortiz, W.: Loss of chloroplast transcripts for proteins associated with photosystem II. An early event during heat-bleaching in Euglena gracilis.-Plant mol. Biol. 27: 317-325, 1995. Go to original source...
    24. Tobin, E.M., Silverthorne, J.: Light regulation of gene expression in higher plants.-Annu. Rev. Plant Physiol. 36: 569-593, 1985. Go to original source...
    25. Zhang, H., Wang, J., Goodman, H.M.: Expression of the Arabidopsis gene Akr coincides with chloroplast development.-Plant Physiol. 106: 1261-1267, 1994. Go to original source...

    Return to the content