Photosynthetica 2002, 40(1):41-48 | DOI: 10.1023/A:1020181804365

Excitation Energy Transfer in Cyanobacteria Synechocystis Embedded in Polymer Film and Partially Bleached by Polarized Irradiation

J. Goc1, K. Klecha1, A. Waskowiak1, J. Miyake2, D. Frackowiak1
1 Faculty of Technical Physics, Institute of Physics, Poznań University of Technology, Poznań, Poland
2 Tissue Engineering Research Center, National Institute for Advanced Industrial Science and Technology, Tsukuba, Japan

The polarized absorption, photoacoustic, fluorescence emission, and fluorescence excitation spectra of whole cells of cyanobacteria Synechocystis sp. embedded in a polymer film were measured. The bacteria cells, as it follows from anisotropy of absorption and fluorescence spectra, were even in a non-stretched polyvinyl alcohol film oriented to a certain extent. The measurements were done for such film in order to avoid the deformation of cyanobacteria shapes. Part of the samples was bleached by irradiation with strong polarized radiation with electric vector parallel to the orientation axis of cells. The anisotropy of photoacoustic spectra was higher than that of absorption spectra and it was stronger changed by the irradiation. Polarized fluorescence was excited in four wavelength regions characterised by different contribution to absorption from various bacteria pigments. The shapes of emission spectra were different depending on wavelength of excitation, polarization of radiation, and previous irradiation of the sample. The fluorescence spectra were analysed on Gaussian components belonging to various forms of pigments from photosystems (PS) 1 and 2. The results inform about excitation energy transfer between pools of pigments, differently oriented in the cells. Energy of photons absorbed by phycobilisomes was transferred predominantly to the chlorophyll of PS2, whereas photons absorbed by carotenoids to chlorophylls of PS1.

Additional key words: absorption spectra; fluorescence spectra; photoacoustic spectra; phycobilisomes; polarized radiation spectroscopy

Published: March 1, 2002  Show citation

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Goc, J., Klecha, K., Waskowiak, A., Miyake, J., & Frackowiak, D. (2002). Excitation Energy Transfer in Cyanobacteria Synechocystis Embedded in Polymer Film and Partially Bleached by Polarized Irradiation. Photosynthetica, 40(1), 41-48. doi: 10.1023/A:1020181804365
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    References

    1. Bialek-Bylka, G.E., Sofrová, D., Szurkowski, J., Skwarek, R., Sopko, B., Manikowski, H.: Linear dichroism, fluorescence polarization, and path of the thermal deactivation of excited cyanobacterial (Synechococcus elongatus) photosystem 1 immobilized and oriented in polymer films.-Photosynthetica 38: 143-148, 2000. Go to original source...
    2. Brody, S.S., Papageorgiou, G., Alygizaki-Zorba, K.: Photodynamic action of hypericin on cyanobacteria Synechocystis and Synechoccus (Anacystis nidulans).-Z. Naturforsch. 52c: 165-168, 1997. Go to original source...
    3. Castenholtz, R.W.: Culturing methods for cyanobacteria.-Meth. Enzymol. 167: 68-93, 1988. Go to original source...
    4. Connolly, J.P., Müller, M.G., Bassi, R., Croce, R., Holtzwarth, A.R.: Femtosecond transient absorption study of carotenoid to chlorophyll energy transfer in the Light-Harvesting Complex II of Photosystem II.-Biochemistry 36: 281-287, 1997. Go to original source...
    5. Ducharme, D., Tessier, A., Leblanc, R.M.: Design and characteristic of a cell for photoacoustic spectroscopy of condensed matter.-Rev. sci. Instrum. 50: 42-43, 1979. Go to original source...
    6. Erokhina, L.G.: [Composition and content of phycobiliproteins in cells of symbiotic nitrogen fixing cyanobacteria Anabaena azollae from various species of the fern Azolla.]-Fiziol. Rast. 38: 742-753, 1991. [In Russ.]
    7. Frąckowiak, D., Cegielski, R., Abdurakhmanov, I.A.: Excitation energy transfer from carotenoids to bacteriochlorophyll in the bacterium Chromatium minutissimum.-Photosynthetica 25: 621-629, 1991.
    8. Frąckowiak, D., Erokhina, L.G., Picard, G., Leblanc, R.M.: Polarized optical properties of phycobilisomes from Anabaena, Nostoc and Synechococcus.-Photochem. Photobiol. 46: 277-285, 1987. Go to original source...
    9. Frąckowiak, D., Gantt, E., Hotchandani, S., Lipschultz, A.C., Leblanc, R.M.: Polarized photoacoustic spectra of phycobilisomes in polyvinyl alcohol films.-Photochem. Photobiol. 43: 335-337, 1986a. Go to original source...
    10. Frąckowiak, D., Hotchandani, S., Szych, B., Leblanc, R.M.: Polarized photoacoustic spectroscopy of oriented systems.-Acta phys. polon. A69: 121-133, 1986b.
    11. Frąckowiak, D., Planner, A.: Spectral properties of cyanobacteria adapted to green and red light.-Acta Physiol. Plant. 17: 133-144, 1995.
    12. Frąckowiak, D., Zelent, B., Malak, H., Cegielski, R., Goc, J., Niedbalska, M., Ptak, A.: Interactions between chlorophyll a and β-carotene in nematic liquid crystals.-Biophys. Chem. 54: 95-107, 1995. Go to original source...
    13. Frank, H.A., Cogdell, R.J.: Carotenoids in photosynthesis.-Photochem. Photobiol. 63: 257-264, 1996. Go to original source...
    14. Gantt, E.: Phycobilisomes.-Annu. Rev. Plant Physiol. 32: 327-347, 1981. Go to original source...
    15. Glazer, A.N.: Phycobilisomes: assembly and attachment.-In: Fay, P., Van Baalen, C. (ed.): Cyanobacteria. Pp. 69-94. Elsevier, Amsterdam-New York-Oxford 1987.
    16. Goedheer, J.C.: Energy transfer from carotenoids to chlorophyll in blue-green, red and green algae and greening bean leaves.-Biochim. biophys. Acta 172: 252-265, 1969. Go to original source...
    17. Grudziński, W., Matuła, M., Sielewiesiuk, J., Kernen, P., Krupa, Z., Gruszecki, W.I.: Effect of 13-cis violaxanthin on organization of light harvesting complex II in monomolecular layers.-Biochim. biophys. Acta 1503: 291-302, 2001. Go to original source...
    18. Goc, J., Klecha, K.: The polarized photoacoustic spectra of Rhodobacter sphaeroides cells embedded in polymer and strongly irradiated by polarized radiation.-Photosynthetica 39: 461-465, 2001. Go to original source...
    19. Hara, M., Miyake, J., Goc, J., Frąckowiak, D.: Photoreaction and thermal deactivation of excitation in purple bacteria light harvesting complexes (LH2) with and without reaction centres.-J. Photochem. Photobiol. A 124: 15-21, 1999. Go to original source...
    20. Kramer, H.J.M., Amesz, J.: Anisotropy of the emission and absorption bands of spinach chloroplasts measured by fluorescence polarization and polarized excitation spectra at low temperature.-Biochim. biophys. Acta 682: 201-207, 1982. Go to original source...
    21. Litvin, F.F., Sineshchekov, V.A.: Molecular organization of chlorophyll and energetics of the initial stages in photosynthesis.-In: Govindjee (ed.): Bioenergetics of Photosynthesis. Pp. 619-661. Academic Press, New York-San Francisco-London 1975. Go to original source...
    22. Lorrain, L., Frąckowiak, D., Romanowski, M., Leblanc, R.M.: Effect of stretching of chloroplasts and thylakoids embedded in polymer films.-Photosynthetica 21: 43-50, 1987.
    23. Mac Coll, R., Guard-Friar, D.: Phycobiliproteins.-Pp. 19-21. CRS Press, Boca Raton 1987.
    24. Martyński, T., Frąckowiak, D., Miyake, J., Dudkowiak, A., Piechowiak, A.: The orientation of bacteriochlorophyll c in green bacteria cells and cell fragments.-J. Photochem. Photobiol. B 42: 57-66, 1998. Go to original source...
    25. Michl, J., Thulstrup, E.W.: Spectroscopy With Polarized Light: Solute Alignment by Photoselection in Liquid Crystals, Polymer and Membranes.-VCH Publishers, New York 1986.
    26. Mimuro, M.: Studies on excitation energy flow in photosynthetic pigment system; structure and energy transfer mechanism.-Bot. Mag. (Tokyo) 103: 233-253, 1990. Go to original source...
    27. Nishigaki, A., Ohsima, S., Nakagama, K., Okada, M., Nagashima, U.: Application of molecular orbital calculations to interpret the chlorophyll spectral forms in pea Photosystem II.-Photochem. Photobiol. 73: 245-248, 2001. Go to original source...
    28. Okhi, K., Okabe, Y., Murakami A., Fujita Y.: Action of near UV and blue light on the photocontrol of phycobiliprotein formation: A complementary chromatic adaptation.-Plant Cell Physiol. 23: 651-656, 1982.
    29. Olson, J.M., Stanton, E.K.: Absorption and fluorescence spectra of bacterial chlorophyll in situ.-In: Vernon, L.P., Seely, R.G. (ed.): The Chlorophylls. Pp. 381-396. Academic Press, New York-London 1966. Go to original source...
    30. Papageorgiou, G.C., Govindjee, Govindjee, R., Mimuro, M., Stamatakis, K., Alygizaki-Zorba, A., Murata, N.: Light-induced and osmotically-induced changes in chlorophyll a fluorescence in two Synechocystis sp. PCC 6803 strains that differ in membrane lipid unsaturation.-Photosynth. Res. 59: 125-136, 1999. Go to original source...
    31. Peterman, E.J.G., Monshouwer, R., van Stokkum, I.H.M., van Grondelle, R., van Amerongen, A.: Ultrafast singlet excitation transfer from carotenoids to chlorophylls via different pathways in light-harvesting complex II of higher plants.-Chem. Phys. Lett. 264: 279-284, 1997. Go to original source...
    32. Skibiński, A., Marcotte, L., Erokhina, L.G., Romanowski, M., Frąckowiak, D.: Spectral properties of partially dissociated and immobilized phycobilisomes obtained from various cyanobacteria.-Photosynthetica 24: 243-248, 1990.
    33. Stamatakis, K., Papageorgiou, G.C.: Phycobilisome-to-photosystem I excitation transfer is enhanced in water-depleted cells and depressed in water replete cells of cyanobacterium Synechococcus sp. PCC7942.-In: Argyroudi-Akoyunoglou, J.H., Senger, H.: Chloroplasts: From Molecular Biology to Biotechnology. Pp. 47-54. Kluwer Academic Publ., Dordrecht-Boston-London 1999. Go to original source...
    34. Tapie, P., Choquet, Y., Breton, J., Delepelaire, P., Wollman, F.A.: Orientation of photosystem I pigments. Investigation by low-temperature linear dichroism and polarized fluorescence emission.-Biochim. biophys. Acta 767: 57-69, 1984. Go to original source...
    35. Tapie, P., Choquet, Y., Wollman, F.-A., Diner, B., Breton, J.: Orientation of the pigments in Photosystem II: a low-temperature linear dichroism and polarized fluorescence emission study of chlorophyll-protein complexes isolated from Chlamydomonas reinhardtii.-Biochim. biophys. Acta 850: 156-161, 1986. Go to original source...

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