Photosynthetica 2002, 40(1):71-75 | DOI: 10.1023/A:1020198308000

Genetic and Functional Characterisation of Rhodobacter Capsulatus Reaction Centres Carrying Triple Mutations Near to or Far from Quinone Sites

S. Ozkan1
1 Faculty of Sciences, Department of Biology, Gebze Institute of Technology, Kocaeli, Turkey

In Rhodobacter capsulatus, two triple mutants were constructed. In these non-photosynthetic mutants, two amino acids near the quinone QB have been mutated to two alanines: in the QA site have been mutated to alanine-aspartic acid and glutamic acid-alanine. Several spontaneous mutants derived from original constructs were selected. DNA sequencing experiments on originally designed mutant strains and their spontaneous mutants were performed to identify possible genetic reversions at quinone site-specific locations. Constructed mutants carry double alanines in the QB site and single alanine in the QA site. Spontaneous mutants carry additional compensating mutations, aspartic acid (L225), cysteine (M231), and serine (M231) far from QA and QB sites, which may be involved in quinone binding by the photosynthetic reaction centres.

Additional key words: DNA sequencing; L and M subunits; spontaneous mutants; sub-cloning

Published: March 1, 2002  Show citation

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Ozkan, S. (2002). Genetic and Functional Characterisation of Rhodobacter Capsulatus Reaction Centres Carrying Triple Mutations Near to or Far from Quinone Sites. Photosynthetica, 40(1), 71-75. doi: 10.1023/A:1020198308000
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