Photosynthetica 2002, 40(2):215-220 | DOI: 10.1023/A:1021341623339

Saturating Irradiance-Induced Photoinhibition without Monomerisation of Photosystem 2 Dimer in Soybean Leaves

Shi-Qing Cai1, Gen-Yun Chen1, Hai-Bo Zhang1, Da-Quan Xu1
1 Chinese Academy of Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Shanghai, China

The oligomeric state of photosystem 2 (PS2) complex in soybean leaves treated with saturating irradiance was studied by non-denaturing polyacrylamide gel electrophoresis (PAGE) and gel filtration chromatography. PS2 dimers resolved by non-denaturing PAGE accounted for about 75 % of total PS2 complex and there was no significant difference in the ratio of PS2 dimer to monomer between samples from saturating irradiance-treated and fully dark-adapted leaves. Furthermore, BBY particles were resolved into four chlorophyll-enriched fractions by gel filtration chromatography. From their molecular masses and protein components, these fractions were deduced to be PS2 dimer, PS2 monomer, oligomeric light-harvesting complex 2 (LHC2), and monomeric LHC2. Also, no change in the proportion of PS2 dimer in total PS2 was observed in the granal region of thylakoid membranes from soybean leaves after saturating irradiation. Hence the dimer is the predominant natural form of PS2 in vivo and no monomerisation of PS2 dimer occurs during saturating irradiance-induced photoinhibition in soybean leaves.

Additional key words: dimer; Glycine max; light-harvesting complex 2; monomerisation; photosystem 2; proteins

Published: June 1, 2002  Show citation

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Cai, S., Chen, G., Zhang, H., & Xu, D. (2002). Saturating Irradiance-Induced Photoinhibition without Monomerisation of Photosystem 2 Dimer in Soybean Leaves. Photosynthetica, 40(2), 215-220. doi: 10.1023/A:1021341623339
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