Photosynthetica 2003, 41(1):151-156 | DOI: 10.1023/A:1025889219115

Photoinhibition and Active Oxygen Species Production in Detached Apple Leaves During Dehydration

H.S. Jia1,2, Y.Q. Han3, D.Q. Li2,4
1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China
2 College of Life Sciences, Shandong Agricultural University, cShandong Tai'an, China
3 Acta Botanica Sinica Editorial Office, Institute of Botany, Chinese Academy of Sciences, Beijing, China
4 Department of Biology, Liaocheng University, Liaocheng, China

In the course of dehydration, the gas exchange and chlorophyll (Chl) fluorescence were measured under irradiance of 800 μmol m-2 s-1 in detached apple leaves, and the production of active oxygen species (AOS), hydrogen peroxide (H2O2), superoxide (O2-), hydroxyl radical (-OH), and singlet oxygen (1O2), were determined. Leaf net photosynthetic rate (PN) was limited by stomatal and non-stomatal factors at slight (2-3 h dehydration) and moderate (4-5 h dehydration) water deficiency, respectively. Photoinhibition occurred after 3-h dehydration, which was defined by the decrease of photosystem 2 (PS2) non-cyclic electron transport (P-rate). After 2-h dehydration, an obvious rise in H2O2 production was found as a result of photorespiration rise. If photorespiration was inhibited by sodium bisulfite (NaHSO3), the rate of post-irradiation transient increase in Chl fluorescence (Rfp) was enhanced in parallel with a slight decline in P-rate and with an increase in Mehler reaction. At 3-h dehydration, leaf P-rate decrease could be blocked by glycine (Gly) or methyl viologen (MV) pre-treatment, and MV was more effective than Gly at moderate drought time. AOS (H2O2 and O2-), prior to photoinhibition produced from photorespiration and Mehler reaction in detached apple leaves at slight water deficiency, were important in dissipating photon energy which was excess to the demand of CO2 assimilation. So photoinhibition could be effectively prevented by the way of AOS production.

Additional key words: chlorophyll fluorescence; gas exchange; irradiance; Malus pumila; Mehler reaction; photorespiration; photosystem 2 non-cyclic electron transport

Published: March 1, 2003  Show citation

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Jia, H.S., Han, Y.Q., & Li, D.Q. (2003). Photoinhibition and Active Oxygen Species Production in Detached Apple Leaves During Dehydration. Photosynthetica, 41(1), 151-156. doi: 10.1023/A:1025889219115
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    References

    1. Asada, K.: Production and action of active oxygen species in photosynthetic tissues.-In: Foyer, C.H., Mullineaux, P.M. (ed.): Causes of Photooxidative Stress and Amelioration of Defense System in Plants. Pp. 77-104. CRC Press, Boca Raton-Ann Arbor-London-Tokyo 1994. Go to original source...
    2. Asada, K.: The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons.-Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 601-639, 1999. Go to original source...
    3. Björkman, O.: High-irradiance stress in higher plants and interaction with other stress factors.-In: Biggins, J. (ed.): Progress in Photosynthesis Research. Vol. 4. Pp. 11-18. Martinus Nijhoff Publishers, Dordrecht-Boston-Lancaster 1987. Go to original source...
    4. Bowler, C., Fluhr, R.: The role of calcium and activated oxygens as signals for controlling cross-tolerance.-Trends Plant Sci. 5: 241-246, 2000. Go to original source...
    5. Chakraborty, N., Tripathy, B.C.: Involvement of singlet oxygen in 5-aminolevulinic acid-induced photodynamic damage of cucumber (Cucumis sativus L.) chloroplasts.-Plant Physiol. 98: 7-11, 1992. Go to original source...
    6. Chandra, S., Stennis, M., Low, P.S.: Measurement of Ca2+ fluxes during elicitation of the oxidative burst in aequorin-transformed tobacco cells.-J. biol. Chem. 272: 8272-8280, 1997. Go to original source...
    7. Chen, W.P., Li, P.H.: Chilling-induced Ca2+ overload enhances production of active oxygen species in maize (Zea mays L.) cultured cells: the effect of abscisic acid treatment.-Plant Cell Environ. 24: 791-800, 2001. Go to original source...
    8. Elstner, E.F.: Mechanism of oxygen activation in different compartments.-In: Pell, E.J., Steffen, K.L. (ed.): Active Oxygen/Oxidative Stress and Plant Metabolism. Pp. 13-25. American Society of Plant Physiologists, Roseville 1991.
    9. Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and photosynthesis.-Annu. Rev. Plant Physiol. 33: 317-345, 1982. Go to original source...
    10. Foyer, C.H., Noctor, G.: Oxygen processing in photosynthesis: regulation and signaling.-New Phytol. 146: 359-388, 2000. Go to original source...
    11. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll florescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    12. Hsiao, T.C.: Plant response to water stress.-Annu. Rev. Plant Physiol. 24: 519-570, 1973. Go to original source...
    13. Jia, H., Li, D.: Relationship between photosystem 2 electron transport and photosynthetic CO2 assimilation responses to irradiance in young apple tree leaves.-Photosynthetica 40: 139-144, 2002. Go to original source...
    14. Jia, H.S., Li, D.Q.: H2O2 metabolism of apple leaves under water stress.-Acta phytophysiol. sin. 27: 321-324, 2001.
    15. Krause, G.H.: The role of oxygen in photoinhibition of photosynthesis.-In: Foyer, C.H., Mullineaux, P.M. (ed.): Causes of Photooxidative Stress and Amelioration of Defense System in Plants. Pp. 43-76. CRC Press, Boca Raton-Ann Arbor-London-Tokyo 1994. Go to original source...
    16. Levine, A., Tenhaken, R., Dixon, R., Lamb, C.: H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response.-Cell 79: 583-593, 1994. Go to original source...
    17. Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633-662, 1994. Go to original source...
    18. Martin, R.E., Thomas, D.J., Tucker, D.E., Herbert, S.K.: The effects of photooxidative stress on photosystem 1 measured in vivo in Chlamydomonas.-Plant Cell Environ. 20: 1451-1461, 1997. Go to original source...
    19. Mi, H., Endo, T., Qgawa, T., Asada, K.: Thylakoid membrane-bond, NADP-specific pyridine nucleotide dehydrosgenase complex mediates cyclic electron transport in the cyanobacterium Synechocystis sp. PCC 6803.-Plant Cell Physiol. 36: 661-668, 1995.
    20. Mukherjee, S.P., Choudhuri, M.R.: Implications of water stress-induced changes in the level of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings.-Physiol. Plant. 58: 166-174, 1983. Go to original source...
    21. Neunenschwander, V., Vernooij, B., Friedrich, L.: Is hydrogen peroxide a second messenger of salicylic acid in systemic acquired resistance?-Plant J. 8: 227-233, 1995. Go to original source...
    22. Price, A.H., Atherton, N.M., Hendry, G.A.F.: Plants under drought-stress generate activated oxygen.-Free Radical Res. Commun. 8: 61-66, 1989. Go to original source...
    23. Sakaki, T., Kondo, N., Sugahara, K.: Breakdown of photosynthetic pigments and lipids in spinach leaves with ozone fumigation: Role of active oxygens.-Physiol. Plant. 59: 28-34, 1983. Go to original source...
    24. Scandalios, J.G.: Oxygen stress and superoxide dismutases.-Plant Physiol. 101: 7-12, 1993. Go to original source...
    25. Schneider, K., Schlegel, H.G.: Production of superoxide radicals by soluble hydrogenase from Alcaligenes eutrophus.-Biochem. J. 193: 99-107, 1981. Go to original source...
    26. Steiner, M.G., Babbs, C.F.: Quantitation of the hydroxyl radical by reaction with dimethyl sulfoxide.-Arch. Biochem. Biophys. 278: 478-481, 1990. Go to original source...
    27. Wise, R.R., Naylor, A.W.: Chilling-enhanced photooxidation. The peroxidative destruction of lipids during chilling injury to photosynthesis and ultrastructure.-Plant Physiol. 83: 272-277, 1987. Go to original source...

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