Photosynthetica 2003, 41(2):233-239 | DOI: 10.1023/B:PHOT.0000011956.29950.97

Contrasting Changes of Photosystem 2 Efficiency in Arabidopsis Xanthophyll Mutants at Room or Low Temperature Under High Irradiance Stress

Chang-Lian Peng1,*, A.M. Gilmore2
1 South China Institute of Botany, Chinese Academy of Sciences, Guangzhou, P.R. China
2 Ecosystem Dynamics Group, Research School of Biological Sciences, Institute of Advanced Studies, Australian National University, Canberra, Australia

We compared the responses of wild type (WT) and three mutants including npq1 (lutein-replete and violaxanthin deepoxidase-deficient), lut2 (lutein-deficient), and lut2-npq1 (double mutant) to high irradiance (HI, 2 000 μmol m-2 s-1) at both low (LT, 5 °C) and room (25 °C) temperature. Xanthophyll-dependent energy dissipation was highest in the WT, followed by the lut2, npq1, and npq1-lut2. At 25 °C the relative stress tolerance expressed by Fv/Fm was consistent with the energy dissipation capacity for the first 2 h of treatment. After 3-4 h, the Fv/Fm levels in lut2 and npq1 converged. Under combined LT and HI the relative tolerance sequence was in contrast to the energy dissipation capacity being WT > npq1> lut2 > lut2-npq1. There were little or no significant change in the contents of xanthophylls and carotenes or the chlorophyll (Chl) a/b ratio in any of the materials. Thus lutein (L) substitution possibly alters the conformation/organisation of L binding proteins to enhance damage susceptibility under HI at LT. The enhanced vulnerability is not compensated for the energy dissipation capacity in the lut2 background at LT.

Additional key words: antheraxanthin; β-carotene; chlorophyll; fluorescence; light-harvesting complex; lutein; neoxanthin; violaxanthin; zeaxanthin

Published: June 1, 2003  Show citation

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Peng, C., & Gilmore, A.M. (2003). Contrasting Changes of Photosystem 2 Efficiency in Arabidopsis Xanthophyll Mutants at Room or Low Temperature Under High Irradiance Stress. Photosynthetica, 41(2), 233-239. doi: 10.1023/B:PHOT.0000011956.29950.97
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