Photosynthetica 2003, 41(4):571-578 | DOI: 10.1023/B:PHOT.0000027522.35486.f6

Moderately Elevated Temperature Eliminates Resistance of Rice Plants with Enhanced Expression of Glutathione Reductase to Intensive Photooxidative Stress

R. Kouřil1, D. Lazár1, H. Lee2, J. Jo2,*, J. Nauš1
1 Laboratory of Biophysics, Faculty of Science, Palacký University, tř, Olomouc, Czech Republic
2 Department of Animal Science, Kyungpook National University, Daegu, South Korea

We developed transgenic rice plants (Oryza sativa L. cv. Daeribbyeo) overproducing cytosolic glutathione reductase (GR) using a GR gene from Brassica campestris and studied their response to photo-oxidative stress in the presence of methyl viologen (MV, 10 and 50 μM concentrations) under room (25 °C) and moderately elevated (35 °C) temperature by analysis of chlorophyll (Chl) a fluorescence parameters (FV/FM, qN, and qP) and of Chl content. Elevated temperature enhanced and accelerated the photo-oxidative damage to photosynthetic apparatus expressed mainly by a fast decrease of qN. Higher temperature supported the protective reaction in transformed rice plants for lower MV concentration (10 μM) and eliminated the enhanced tolerance of photosystem 2 photochemistry to photooxidative stress for higher (50 μM) MV concentration. Different mechanisms and temperature dependence of oxidative and protective reactions explain the results.

Additional key words: active oxygen species; chlorophyll fluorescence; Oryza sativa; photochemical and non-photochemical fluorescence quenching; transgenic plants

Published: December 1, 2003  Show citation

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Kouřil, R., Lazár, D., Lee, H., Jo, J., & Nauš, J. (2003). Moderately Elevated Temperature Eliminates Resistance of Rice Plants with Enhanced Expression of Glutathione Reductase to Intensive Photooxidative Stress. Photosynthetica, 41(4), 571-578. doi: 10.1023/B:PHOT.0000027522.35486.f6
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