Photosynthetica 2004, 42(2):193-199 | DOI: 10.1023/B:PHOT.0000040590.10919.02

Interaction of Photosystem 2-LHC2 Supercomplexes in Adjacent Layers of Stacked Chloroplast Thylakoid Membranes

L. Bumba1,2, M. Hušák3, F. Vácha2,3
1 Faculty of Biological Sciences, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic
2 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic, e-mail
3 Institute of Physical Biology, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic

Two Types of photosystem 2-light-harvesting complex 2 (PS2-LHC2) supercomplexes with similar pigment and protein composition were isolated directly from thylakoid membranes by sucrose density gradient centrifugation. Electron microscopy and single particle image analysis revealed the first Type as single unpaired PS2-LHC2 supercomplexes, whereas the second Type was characterized as pairs of two PS2-LHC2 supercomplexes attached together by their stromal sides. Unstacking of thylakoid membranes resulted in a spontaneous disintegration of the paired supercomplexes into single unpaired particles. A model of the organisation of the pigment-protein complexes in grana region is proposed.

Additional key words: electron microscopy; membrane proteins; model; photosynthesis; photosystem 2; Pisum; single particle image analysis

Published: June 1, 2004  Show citation

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Bumba, L., Hušák, M., & Vácha, F. (2004). Interaction of Photosystem 2-LHC2 Supercomplexes in Adjacent Layers of Stacked Chloroplast Thylakoid Membranes. Photosynthetica, 42(2), 193-199. doi: 10.1023/B:PHOT.0000040590.10919.02
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    References

    1. Allen, J.F., Forsberg, J.: Molecular recognition in thylakoid structure and function.-Trends Plant Sci. 6: 317-326, 2001. Go to original source...
    2. Andersson, B., Anderson, J.M.: Lateral heterogeneity in the dis-tribution of chlorophyll-protein complexes of the thylakoid membranes of spinach chloroplasts.-Biochim. biophys. Acta 593: 427-440, 1980. Go to original source...
    3. Barber, J.: Membrane surface charges and potentials in relation to photosynthesis. - Biochim. biophys. Acta 594: 253-308, 1980. Go to original source...
    4. Barber, J., Mills, J.: Control of chlorophyll fluorescence by the diffuse double layer.-FEBS Lett. 68: 288-292, 1976. Go to original source...
    5. Barber, J., Nield, J., Morris, E.P., Zheleva, D., Hankamer, B.: The structure, function and dynamics of photosystem two.-Physiol. Plant. 100: 817-827, 1997. Go to original source...
    6. Boekema, E.J., Hankamer, B., Bald, D., Kruip, J., Nield, J., Boonstra, A.F., Barber, J., Rögner, M.: Supramolecular struc-ture of the photosystem II complex from green plants and cyanobacteria.-Proc. nat. Acad. Sci. USA 92: 175-179, 1995. Go to original source...
    7. Boekema, E.J., Jensen, P.E., Schlodder, E., van Breemen, J.F.L., van Roon, H., Scheller, V.H., Dekker, J.P.: Green plant photosystem I binds light-harvesting complex I on one side of the complex.-Biochemistry 40: 1029-1036, 2001. Go to original source...
    8. Boekema, E.J., Nield, J., Hankamer, B., Barber, J.: Localization of the 23-kDa subunit of the oxygen evolving complex of photosystem II by electron-microscopy.-Eur. J. Biochem. 252: 268-276, 1998a. Go to original source...
    9. Boekema, E.J., van Breemen, J.F.L., van Roon, H., Dekker, J.P.: Arrangement of photosystem II supercomplexes in crys-talline macrodomains within the thylakoid membrane of green plant chloroplasts.-J. mol. Biol. 301: 1123-1133, 2000. Go to original source...
    10. Boekema, E.J., van Roon, H., Calkoen, F., Bassi, R., Dekker, J.P.: Multiple Types of association of photosystem II and its light-harvesting antenna in partially solubilized photosystem II membranes.-Biochemistry 38: 2233-2239, 1999. Go to original source...
    11. Boekema, E.J., van Roon, H., Dekker, J.P.: Specific association of photosystem II and light-harvesting complex II in partially solubilized photosystem II membranes.-FEBS Lett. 424: 95-99, 1998b. Go to original source...
    12. Bumba, L., Vácha, F.: Electron microscopy in structural studies of Photosystem II.-Photosynth. Res. 77: 1-19, 2003. Go to original source...
    13. Chow, W.S., Miller, C., Anderson, J.M.: Surface charges, the heterogenous lateral distribution of the two photosystems, and thylakoid stacking.-Biochim. biophys. Acta 1057: 69-77, 1991. Go to original source...
    14. Eshaghi, S., Andersson, B., Barber, J.: Isolation of a highly active PS2-LHC2 supercomplex from thylakoid membranes by a direct method.-FEBS Lett. 446: 23-26, 1999. Go to original source...
    15. Frank, J., Radermacher, M., Penczek, P., Zhu, J., Li, Y.H., Ladjadj, M., Leith, A.: SPIDER and WEB: Processing and vi-sualization of images in 3D electron microscopy and related fields.-J. struct. Biol. 116: 190-199, 1996. Go to original source...
    16. Green, B.R., Durnford, D.G.: The chlorophyll-carotenoid pro-teins of oxygenic photosynthesis.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 685-714, 1996. Go to original source...
    17. Gross, E.L., Zimmermann, R.J., Hormats, G.F.: The effect of mono-and divalent cations on the quantum yields for electron transport in chloroplasts.-Biochim. biophys. Acta 440: 59-67, 1976. Go to original source...
    18. Hankamer, B., Barber, J., Boekema, E.J.: Structure and mem-brane organization of photosystem II in green plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 48: 641-671, 1997a. Go to original source...
    19. Hankamer, B., Morris, E.P., Nield, J., Carne, A., Barber, J.: Subunit positioning and transmembrane helix organisation in the core dimer of photosystem II.-FEBS Lett. 504: 142-151, 2001a. Go to original source...
    20. Hankamer, B., Morris, E., Nield, J., Gerle, C., Barber, J.: Three-dimensional structure of the photosystem II core dimer of higher plants determined by electron microscopy.-J. struct. Biol. 135: 262-269, 2001b. Go to original source...
    21. Hankamer, B., Nield, J., Zheleva, D., Boekema, E., Jansson, S., Barber, J.: Isolation and biochemical characterization of mo-nomeric and dimeric photosystem II complexes from spinach and their relevance to the organization of photosystem II in vivo.-Eur. J. Biochem. 243: 422-429, 1997b. Go to original source...
    22. Harauz, G., Boekema, E., van Heel, M.: Statistical image analysis of electron micrographs of ribosomal subunits.-Methods Enzymol. 164: 35-49, 1988. Go to original source...
    23. Harrer, R., Bassi, R., Testi, M.G., Schafer, C.: Nearest-neighbor analysis of a photosystem II complex from Marchantia polymorpha L. (liverwort), which contains reaction center and antenna proteins.-Eur. J. Biochem. 255: 196-205, 1998. Go to original source...
    24. Izawa, S., Good, N.E.: Effect of salts and electron transport on the conformation of isolated chloroplasts. II. Electron micro-scopy.-Plant Physiol. 41: 544-552, 1966. Go to original source...
    25. Jennings, R.C., Forti, G.: The influence of magnesium on the chlorophyll fluorescence yield of isolated chloroplasts.-Biochim. biophys. Acta 347: 299-310, 1974. Go to original source...
    26. Kamiya, N., Shen, J.R.: Crystal structure of oxygen-evolving photosystem II from Thermosynechococcus vulcanus at 3.7-angstrom resolution.-Proc. nat. Acad. Sci. USA 100: 98-103, 2003. Go to original source...
    27. Kühlbrandt, W., Wang, D.N., Fujiyoshi, Y.: Atomic model of plant light-harvesting complex by electron crystallography.-. Nature 367: 614-621, 1994. Go to original source...
    28. Laemmli, U.K.: Cleavage of structural proteins during the as-sembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    29. Murata, N.: Control of excitation transfer in photosynthesis. II. Magnesium ion-dependent distribution of excitation energy between two pigment systems in spinach chloroplasts.-Biochim. biophys. Acta 189: 171-181, 1969. Go to original source...
    30. Mustardy, L., Garab, G.: Granum revisited. A three-dimensional model-where things fall into place.-Trends Plant Sci. 8: 117-122, 2003. Go to original source...
    31. Nield, J., Orlova, E.V., Morris, E.P., Gowen, B., van Heel, M., Barber, J.: 3D map of the plant photosystem II supercomplex obtained by cryoelectron microscopy and single particle ana-lysis.-Nature struct. Biol. 7: 44-47, 2000. Go to original source...
    32. Scheller, H.V., Jensen, P.E., Haldrup, A., Lunde, C., Knoetzel, J.: Role of subunits in eukaryotic Photosystem I.-Biochim. biophys. Acta 1507: 41-60, 2001. Go to original source...
    33. Schmid, V.H.R., Potthast, S., Wiener, M., Bergauer, V., Paulsen, H., Storf, S.: Pigment binding of photosystem I light-harvesting proteins.-J. biol. Chem 277: 37307-37314, 2002. Go to original source...
    34. Staehelin, L.A.: Reversible particle movements associated with unstacking and restacking of chloroplast membranes in vitro.-J. Cell Biol. 71: 136-158, 1976. Go to original source...
    35. Telfer, A., Nicolson, J., Barber, J.: Cation control of chloroplast structure and chlorophyll a fluorescence yield and its rele-vance to the intact chloroplast.-FEBS Lett. 65: 77-83, 1976. Go to original source...
    36. Vácha, F., Vácha, M., Bumba, L., Hashizume, K, Tani, T.: Inner structure of intact chloroplasts observed by a low tem-perature laser scanning microscope.-Photosynthetica 38: 493-496, 2000. Go to original source...
    37. van Heel, M., Frank, J.: Use of multivariate statistics in ana-lyzing the images of biological macromolecules.-Ultra-microscopy 6: 187-194, 1981. Go to original source...
    38. Yakushevska, A.E., Jensen, P.E., Keegstra, W., van Roon, H., Scheller, H.V., Boekema, E.J., Dekker, J.P.: Supermolecular organization of photosystem II and its associated light-har-vesting antenna in Arabidopsis thaliana.-Eur. J. Biochem. 268: 6020-6028, 2001. Go to original source...
    39. Zouni, A., Witt, H.T., Kern, J., Fromme, P., Krauss, N., Saenger, W., Orth, P.: Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution.-Nature 409: 739-743, 2001. Go to original source...

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