PHOTOSYNTHETICA

Photosynthetica 2005, 43(1):141-146 | DOI: 10.1007/s11099-005-1146-z

Evidence for positive selection in phycoerythrin genes of red algae and cyanobacteria Prochlorococcus and Synechococcus

S. Qin^1,*, F. Q. Zhao^1,2, C. K. Tseng¹

¹ Institute of Oceanology, Chinese Academy of Sciences, Qingdao, P.R. China

² Graduate School of Chinese Academy of Sciences, Qingdao, P.R. China

Because of the shortage of phycoerythrin (PE) gene sequences from rhodophytes, peBA encoding β- and α -subunits of PE from three species of red algae (Ceramium boydenn, Halymenia sinensis, and Plocamium telfariae) were cloned and sequenced. Different selection forces have affected the evolution of PE lineages. 8.9 % of the codons were subject to positive selection within the PE lineages (excluding high-irradiance adapted Prochlorococcus). More than 40 % of the sites may be under positive selection, and nearly 20 % sites are weakly constraint sites in high-irradiance adapted Prochlorococcus. Sites most likely undergoing positive selection were found in the chromophore binding domains, suggesting that these sites have played important roles in environmental adaptation during PE diversification. Moreover, the heterogeneous distribution of positively selected sites along the PE gene was revealed from the comparison of low-irradiance adapted Prochlorococcus and marine Synechococcus, which firmly suggests that evolutionary patterns of PEs in these two lineages are significantly different.

Additional key words: Ceramium boydenn; Halymenia sinensis; molecular evolution; phylogenetic analysis; Plocamium telfariae; positive selection; Synechococcus

Received: July 1, 2004; Accepted: October 11, 2004; Published: March 1, 2005  Show citation