Photosynthetica 2005, 43(2):211-216 | DOI: 10.1007/PL00022057

Long-term effect of elevated CO2 on spatial differentiation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity in Norway spruce canopy

M. Hrstka1, O. Urban2,*, M. V. Marek2,3
1 Faculty of Chemistry, Brno University of Technology, Brno, Czech Republic
2 Laboratory of Ecological Physiology of Forest Trees, Institute of Landscape Ecology, Academy of Sciences of the Czech Republic, Brno, Czech Republic
3 Institute of Physical Biology, University of South Bohemia, Nove Hrady, Czech Republic

Total in vitro activity of RuBPCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) enzyme was assayed spectrophotometrically by the continuous measurement of 3-phosphoglycerate-dependent NADH oxidation in a coupled enzyme system. RuBPCO activities were found in the ranges 1.01-2.76 and 1.23-3.10 µmol(CO2) m-2 s- 1 in current Norway spruce needles growing in ambient (AC) and elevated (EC) CO2 concentration, respectively. RuBPCO activity in AC needles from the upper layer (U) was 11-15 % higher compared to those from the middle (M) layer, and even 44-56 % higher compared to the lower (L) layer of spruce crown. Over the vegetation season, we observed a highly significant decrease of RuBPCO activity in the EC-U needles from 3.10 (July) to 1.60 (October) µmol(CO2) m-2 s-1 as a consequence of downward feedback regulation. Moreover, this down-regulation was not caused by a non-specific decrease in total leaf nitrogen content.

Additional key words: down-regulation; nitrogen; photosynthesis; Picea abies; sun and shade needles; vertical profile

Received: June 24, 2004; Accepted: January 17, 2005; Published: June 1, 2005  Show citation

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Hrstka, M., Urban, O., & Marek, M.V. (2005). Long-term effect of elevated CO2 on spatial differentiation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity in Norway spruce canopy. Photosynthetica, 43(2), 211-216. doi: 10.1007/PL00022057
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