Photosynthetica 2005, 43(3):403-409 | DOI: 10.1007/s11099-005-0064-4

Plant shading increases lipid peroxidation and intensifies senescence-induced changes in photosynthesis and activities of ascorbate peroxidase and glutathione reductase in wheat

M. Spundova1,*, K. Sloukova1, M. Hunkova1, J. Naus1
1 Department of Experimental Physics, Laboratory of Biophysics, Faculty of Sciences, Palacky University, Olomouc, Czech Republic

Plants of spring wheat (Triticum aestivum L. cv. Saxana) were grown during the autumn. Over the growth phase of three leaves (37 d after sowing), some of the plants were shaded and the plants were grown at 100 (control without shading), 70, and 40 % photosynthetically active radiation. Over 12 d, chlorophyll (Chl) and total protein (TP) contents, rate of CO2 assimilation (PN), maximal efficiency of photosystem 2 photochemistry (FV/FP), level of lipid peroxidation, and activities of antioxidative enzymes ascorbate peroxidase (APX) and glutathione reductase (GR) were followed in the 1st, 2nd, and 3rd leaves (counted according to their emergence). In un-shaded plants, the Chl and TP contents, PN, and FV/FP decreased during plant ageing. Further, lipid peroxidation increased, while the APX and GR activities related to the fresh mass (FM) decreased. The APX activity related to the TP content increased in the 3rd leaves. The plant shading accelerated senescence including the increase in lipid peroxidation especially in the 1st leaves and intensified the changes in APX and GR activities. We suggest that in the 2nd and 3rd leaves a degradation of APX was slowed down, which could reflect a tendency to maintain the antioxidant protection in chloroplasts of these leaves.

Additional key words: ageing; chlorophyll fluorescence; CO2 assimilation; plant topography; Triticum aestivum

Received: February 14, 2005; Accepted: June 2, 2005; Published: September 1, 2005  Show citation

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Spundova, M., Sloukova, K., Hunkova, M., & Naus, J. (2005). Plant shading increases lipid peroxidation and intensifies senescence-induced changes in photosynthesis and activities of ascorbate peroxidase and glutathione reductase in wheat. Photosynthetica, 43(3), 403-409. doi: 10.1007/s11099-005-0064-4
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    References

    1. Asada, K.: The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons.-Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 601-639, 1999. Go to original source...
    2. Asada, K.: The water-water cycle as alternative photon and electron sinks. - Phil. Trans. roy. Soc. London B 355: 1419-1431, 2000. Go to original source...
    3. Buschmann, C.: The characterization of the developing photosynthetic apparatus in greening barley leaves by means of (slow) fluorescence kinetic measurements. - In: Akoyunoglou, G. (ed.): Photosynthesis. Vol. V. Pp. 417-426. Balaban International Science Services, Philadelphia 1981.
    4. Camp, P.J., Huber, S.C., Burke, J.J., Moreland, D.E.: Biochemical changes that occur during senescence of wheat leaves. I. Basis for the reduction of photosynthesis. - Plant Physiol. 70: 1641-1646, 1982. Go to original source...
    5. Dan, K., Nagata, M., Yamashita, I.: Changes in lipid peroxidation and antioxidant content in cotyledons of japanese radish. - J. jap. Soc. hort. Sci. 65: 603-608, 1996. Go to original source...
    6. Feekes, W.: De tarwe en haar milieu'. - Vers. XVII. Pp. 560-561. Tarwe Commun. Groningen 1941.
    7. Feng, Z.Z., Guo, A.H., Feng, Z.W.: Delay od senescence of detached cucumber cotyledons by triadimefon. - Biol. Plant. 46: 571-575, 2003. Go to original source...
    8. Foyer, C.H., Halliwell, B.: The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism. - Planta 133: 21-25, 1976. Go to original source...
    9. Gillham, D.J., Dodge, A.D.: Hydrogen peroxide scavenging systems in pea chloroplasts. - Planta 167: 246-251, 1986. Go to original source...
    10. Gillham, D.J., Dodge, A.D.: Chloroplast superoxide and hydrogen peroxide scavenging systems from pea leaves: seasonal variations. - Plant Sci. 50: 105-109, 1987. Go to original source...
    11. Goel, A., Sheoran, I.S.: Lipid peroxidation and peroxide-scavenging enzymes in cotton seeds under natural ageing.-Biol. Plant. 46: 429-434, 2003. Go to original source...
    12. Grover, A.: How do senescing leaves lose photosynthetic activity. - Curr. Sci. 64: 226-234, 1993.
    13. Jiang, Y., Huang, B.: Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. - Crop Sci. 41: 436-442, 2001. Go to original source...
    14. Jimenez, A., Hernandez, J.A., del Rio, L.A., Sevilla, F.: Evidence for the presence of the ascorbate-glutathione cycle in mitochondria and peroxisomes of pea leaves. - Plant Physiol. 114: 275-284, 1997. Go to original source...
    15. Jing, H.C., Hille, J., Dijkwel, R.R.: Ageing of plants: Conserved strategies and novel pathways. - Plant Biol. 5: 455-464, 2003. Go to original source...
    16. Kanazawa, S., Sano, S., Koshiba, T., Ushimaru, T.: Changes in antioxidative enzymes in cucumber cotyledons during natural senescence: comparison with those during dark-induced senescence. - Physiol. Plant. 109: 211-216, 2000. Go to original source...
    17. Kar, M., Streb, P., Hertwig, B., Feierabend, J.: Sensitivity to photodamage increases during senescence in excised leaves. - J. Plant Physiol. 141: 538-544, 1993. Go to original source...
    18. Lazar, D., Naus, J.: Statistical properties of chlorophyll fluorescence induction parameters. - Photosynthetica 35: 121-127, 1998. Go to original source...
    19. Lichtenthaler, H.K.: Chlorophylls and carotenoids-pigments of photosynthetic biomembranes. - In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987. Go to original source...
    20. Logan, B.A., Demmig-Adams, B., Adams, W.W., III, Grace, S.C.: Antioxidants and xanthophyll cycle-dependent energy dissipation in Cucurbita pepo L. and Vinca major L. acclimated to four growth PPFD in the field. - J. exp. Bot. 49: 1869-1879, 1998. Go to original source...
    21. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-2725, 1951. Go to original source...
    22. Matouskova, M., Naus, J., Flasarova, M.: A long-term response of chlorophyll fluorescence induction to one-shot application of cyanazine on barley plants and its relation to crop yield. - Photosynthetica 37: 281-294, 1999. Go to original source...
    23. Munne-Bosch, S., Allegre, L.: Plant aging increases oxidative stress in chloroplasts. - Planta 214: 608-615, 2002. Go to original source...
    24. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867-880, 1981.
    25. Naus, J., Dvorak, L., Kupka, Z.: Heterogeneity of chlorophyll-a fluorescence parameters of green leaves.-Acta Univ. palack. olomuc. Fac. Rer. nat. 82 (Physica 24): 25-34, 1985.
    26. Sestak, Z. (ed.): Photosynthesis During Leaf Development. - Academia, Praha; Dr W Junk Publishers, Dordrecht-Boston-Lancaster 1985. Go to original source...
    27. Sestak, Z., Siffel, P.: Leaf-age related differences in chlorophyll fluorescence. - Photosynthetica 33: 347-369, 1997.
    28. Spundova, M., Popelkova, H., Ilik, P., Skotnica, J., Novotny, R., Naus, J.: Ultra-structural and functional changes in the chloroplasts of detached barley leaves senescing under dark and light conditions. - J. Plant Physiol. 160: 1051-1058, 2003. Go to original source...
    29. Strasser, R.J., Govindjee: The F0 and the O-J-I-P fluorescence rise in higher plants and algae. - In: Argyroudi-Akoyunoglou, J.H. (ed.): Regulation of Chloroplast Biogenesis. Pp. 423-426. Plenum Press, New York 1992. Go to original source...
    30. Thomas, H., Ougham, H.J., Wagstaff, C., Stead, A.D.: Defining senescence and death. - J. exp. Bot. 54: 1127-1132, 2003. Go to original source...
    31. Yamazaki, J., Kamimura, Y.: Relationship between photosystem stoichiometries and changes in active oxygen scavenging enzymes in natural grown rice seedlings. - Plant Growth Regul. 36: 113-120, 2002. Go to original source...

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