Photosynthetica 2006, 44(2):187-192 | DOI: 10.1007/s11099-006-0005-x

Water stress-sensitized photoinhibition in senescing cotyledons of clusterbean: Changes in thylakoid structures and inactivation of photosystem 2

P. M. Deo2, U. C. Biswal1, B. Biswal1,*
1 Biochemistry Laboratory, School of Life Sciences, Sambalpur University, Orissa, India
2 Department of Botany, Panchayat College, Bargarh, Orissa, India

Seedlings of Cyamopsis tetragonoloba were grown on Petri dishes either in water or water plus 3 % PEG-6000 to induce water stress. The senescing cotyledons experiencing the stress exhibited loss in contents of leaf proteins and chlorophyll (Chl) and a decline in oxygen evolution. The effect of PEG treatment was more pronounced at moderate (MI) than low (LI) irradiance. The stress-induced loss in the activity of superoxide dismutase and increase in the thylakoid lipid peroxidation accompanied a change in the physical status of the bilayer membrane as demonstrated by an enhancement of room temperature Chl a fluorescence polarization and decrease in energy transfer efficiency in pigment assembly. This resulted in a sustained decrease in photosystem 2 activity blocking channels of energy utilization. The absorbed quanta, thus unutilized, were excess even at MI, leading to photoinhibitory response.

Additional key words: Cyamopsis tetragonolobar; fluorescence polarization; lipid peroxidation; malondialdehyde; membrane fluidity; polyethylene glycol; proteins; superoxide dismutase

Received: January 6, 2005; Accepted: August 15, 2005; Published: June 1, 2006  Show citation

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Deo, P.M., Biswal, U.C., & Biswal, B. (2006). Water stress-sensitized photoinhibition in senescing cotyledons of clusterbean: Changes in thylakoid structures and inactivation of photosystem 2. Photosynthetica, 44(2), 187-192. doi: 10.1007/s11099-006-0005-x
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