Photosynthetica 2006, 44(2):262-267 | DOI: 10.1007/s11099-006-0017-6

Role of thermal dissipation in the photoprotection in cucumber plants after exposure to a chill stress

Y. H. Zhou1, W. H. Mao1, Y. Y. Zhang1, L. F. Huang1, W. H. Hu1, J. Q. Yu1,2,*
1 Department of Horticulture, Huajiachi Campus, Zhejiang University, Hangzhou, P.R. China
2 Key Laboratory of Horticultural Plants Growth, Development and Biotechnology, Agricultural Ministry of China, Hangzhou, P.R. China

Experiments were carried out to investigate the changes in CO2 assimilation, photon allocation, and photosynthetic electron flux in leaves of cucumber (Cucumis sativus L.) plants after chilling stress. Chilling significantly decreased CO2 assimilation, the energy flux via linear electron transport (J PS2) and non-constitutive thermal dissipation (J NPQ) but increased fluorescence and constitutive thermal dissipation (J f,D) in chilling-sensitive genotype Jinyan No. 4. In contrast, chilling had little effects on J NPQ and J f,D although CO2 assimilation and J PS2 were inhibited in chilling-tolerant genotype Jinchun No. 3. In parallel with the reduction in J PS2, electron flux to oxygenation and carboxylation by ribulose-1,5-bisphosphate carboxylase/oxygenase all significantly decreased while electron flux to O2 significantly increased, especially in chilling-sensitive genotype. Thermal and fluorescence dissipation were the main energy dissipation pathways whilst water-water cycle was an important electron sink when photosynthetic carbon reduction was suppressed after chilling. Chilling sensitivity of the photosynthetic apparatus was related to the operation of different photoprotection mechanisms.

Additional key words: alternative electron sink; chilling; Cucumis sativus; photosynthesis; photosystem 2

Received: June 20, 2005; Accepted: September 19, 2005; Published: June 1, 2006  Show citation

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Zhou, Y.H., Mao, W.H., Zhang, Y.Y., Huang, L.F., Hu, W.H., & Yu, J.Q. (2006). Role of thermal dissipation in the photoprotection in cucumber plants after exposure to a chill stress. Photosynthetica, 44(2), 262-267. doi: 10.1007/s11099-006-0017-6
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    References

    1. Asada, K.: The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons.-Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 601-639, 1999. Go to original source...
    2. Caemmerer, S. von, Farquhar, G.D.: Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves.-Planta 153: 376-387, 1981. Go to original source...
    3. Cakmak, I., Marschner, H.: Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves.-Plant Physiol. 98: 1222-1227, 1992. Go to original source...
    4. Demmig-Adams, B., Adams, W.W., III., Barker, D.H., Logan, B.A., Bowling, D.R., Verhoeven, A.S.: Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation.-Physiol. Plant. 98: 253-264, 1996. Go to original source...
    5. Finazzi, G., Johnson, G.N., Dall'Osto, L., Joliot, P., Wollman, F., Bassi, R.: A zeaxanthin-independent nonphotochemical quenching mechanism localized in the photosysthem II core complexes.-Proc. nat. Acad. Sci. USA 101: 12375-12380, 2004. Go to original source...
    6. Garcia-Limones, G., Hervas, A., Navas-Cortes, J.A., Jimenez-Diaz, R.M., Tena, M.: Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea (Cicer arietinum L.) and Fusarium oxysporum f. sp. ciceris.-Physiol. mol. Plant Pathol. 61: 325-337, 2002. Go to original source...
    7. Harley, P.C., Loreto, F., di Marco, G., Sharkey, T.D.: Theoretical considerations when estimating the mesophyll conductance to CO2 flux by analysis of the response of photosynthesis to CO2.-Plant Physiol. 98: 1429-1436, 1992. Go to original source...
    8. Heber, U., Bukhov, N.G., Shuvalov, V.A., Kobayashi, Y., Lange, O.L.: Protection of the photosynthetic apparatus against damage by excessive illumination in homoiohydric leaves and poikilohydric mosses and lichens.-J. exp. Bot. 52: 1999-2006, 2001. Go to original source...
    9. Hendrickson, J., Foster, B., Furbank, R.T., Chow, W.S.: Process contributing to photoprotection of grapevine leaves illuminated at low temperature.-Physiol. Plant. 121: 272-281, 2004. Go to original source...
    10. Hirotsu, N., Makino, A., Ushio, A., Mae, T.: Changes in the thermal dissipation and the electron flow in the water-water cycle in rice grown under conditions of physiologically low temperature.-Plant Cell Physiol. 45: 635-644, 2004. Go to original source...
    11. Jung, S.Y.: Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought.-Plant Sci. 166: 459-466, 2004. Go to original source...
    12. Krall, J.P., Edward, G.E.: Relationship between photosystem II activity and CO2 fixation in leaves.-Physiol. Plant. 86: 180-187, 1992. Go to original source...
    13. Kramer, D.M., Johnson, G., Kiirats, O., Edwards, G.E.: New fluorescence parameters for the determination of QA redox state and excitation energy fluxes.-Photosynth. Res. 79: 209-218, 2004. Go to original source...
    14. Laloi, C., Apel, K., Danon, A.: Reactive oxygen signalling: the latest news.-Curr. Opin. Plant Biol. 7: 323-328, 2004. Go to original source...
    15. Leipner, J., Fracheboud, Y., Stamp, P.: Acclimation by suboptimal growth temperature diminishes photooxidative damage in maize leaves.-Plant Cell Environ. 20: 366-372, 1997. Go to original source...
    16. Mithofer, A., Schulze, B., Boland, W.: Biotic and heavy metal stress response in plants: evidence for common signals.-FEBS Lett. 566: 1-5, 2004 Go to original source...
    17. Makino, A., Miyake, C., Yokota, A., Ushio, A., Mae, T.: Physiological functions of the water-water cycle and the cyclic electron flow around PSI in rice including RbcS antisense plants.-Plant Cell Physiol. 44: S14-S14, 2003.
    18. Miyake, C., Yokota, A.: Determination of the rate of photoreduction of O2 in the water-water cycle in watermelon leaves and enhancement of the rate by limitation of photosynthesis.-Plant Cell Physiol. 41: 335-343, 2000. Go to original source...
    19. Ort, D.R., Baker, N.R.: A photoprotective role for O2 as an alternative electron sink in photosynthesis?-Curr. Opin. Plant Biol. 5: 193-198, 2002. Go to original source...
    20. Osmond, B., Badger, M., Maxwell, K., Björkman, O., Leegood, R.: Too many photons: photorespiration, photoinhibition and photooxidation.-Trends Plant Sci. 2: 119-120, 1997. Go to original source...
    21. Prasad, T.K., Anderson, M.D., Martin, B.A., Stewart, C.R.: Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide.-Plant Cell 6: 65-74, 1994. Go to original source...
    22. Rizhsky, L., Liang, H., Mittler, R.: The water-water cycle is essential for chloroplast protection in the absence of stress.-J. biol. Chem. 278: 38921-38925, 2003. Go to original source...
    23. Schnettger, B., Critchley, C., Santore, U.J., Graf, M., Krause, G.H.: Relationship between photoinhibition of photosynthesis, D1 protein turnover and chloroplast structure: effects of protein synthesis inhibitors.-Plant Cell Environ. 17: 55-64, 1994. Go to original source...
    24. Schöner, S., Krause, G.H.: Protective systems against active oxygen species in spinach: response to cold acclimation in excess light.-Planta 180: 383-389, 1990. Go to original source...
    25. Shen, W.Y., Nada, K., Tachibana S.: Oxygen radical generation in chilled leaves of cucumber (Cucumis sativus L.) cultivars with different tolerances to chilling temperature.-J. jap. Soc. hort. Sci. 68: 780-787, 1999. Go to original source...
    26. Thayer, S.S., Björkman, O.: Leaf xanthophyll content and composition in sun and shade determined by HPLC.-Photosynth. Res. 23: 331-343, 1990. Go to original source...
    27. Xu, C.C., Jeon, Y.A., Lee, C.H.: Relative contributions of photochemical and non-photochemical routes to excitation energy dissipation in rice and barley illuminated at a chilling temperature.-Physiol. Plant. 107: 447-453, 1999. Go to original source...
    28. Yu, J.Q., Matsui, Y.: Effects of root exudates and allelo-chemicals on ion uptake by cucumber seedlings.-J. chem. Ecol. 23: 817-827, 1997. Go to original source...
    29. Zhou, Y.H., Huang, L.F., Du, Y.S., Yu, J.Q.: Greenhouse and field cucumber genotypes use different mechanisms to protect against dark chilling.-Funct. Plant Biol. 31: 1215-1223, 2004a. Go to original source...
    30. Zhou, Y.H., Yu, J.Q., Huang, L.F., Nogués, S.: The relationship between CO2 assimilation, photosynthetic electron transport and water-water cycle in chill-exposed cucumber leaves under low light and subsequent recovery.-Plant Cell Environ. 27: 1503-1514, 2004b. Go to original source...
    31. Yu, J.Q., Zhou, Y.H., Huang, L.F., Allen, D.: Chill-induced inhibition of photosynthesis: genotypic variation within Cucumis sativus.-Plant Cell Physiol. 43: 1182-1188, 2002. Go to original source...

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