Photosynthetica 2006, 44(3):474-477 | DOI: 10.1007/s11099-006-0054-1

Low iron stress nutrition for evaluation of Fe-efficient genotype physiology, photosynthesis, and essential monoterpene oil(s) yield of Ocimum sanctum

A. Misra1,*, S. Dwivedi1, A. K. Srivastava1, D. K. Tewari1, A. Khan1, R. Kumar1
1 Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, India

Genotypic variations were observed among 6 variants and an indigenous one as a control. An efficient genotype OCD(L) of Ocimum sanctum had the greatest content of chlorophylls [2.15 g kg-1(FM)], Chl a/b ratio of 1.86, net photosynthetic rate of 0.72 mg(CO2) m-2 s-1, and 1.56 % oil formation. Methyl chavicol formed 50.31 % of total oil in OSP-6 genotype, which had also maximum peroxidase activity [ΔOD 2.4 mg-1(protein)] and maximum production of eugenol (30.44 % of total oil). We found an oxido-reducible reaction of peroxidase and high bands of peroxidase isoenzymes in this OSP-6 genotype for the formation of monoterpene essential oil(s) and possibly the major constituents of eugenol through the high production of photosynthates.

Additional key words: chlorophyll; Cu; dry mass; Fe; leaf area; Mn; net photosynthetic rate; plant height; saccharides; Zn

Received: November 21, 2005; Accepted: February 2, 2006; Published: September 1, 2006  Show citation

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Misra, A., Dwivedi, S., Srivastava, A.K., Tewari, D.K., Khan, A., & Kumar, R. (2006). Low iron stress nutrition for evaluation of Fe-efficient genotype physiology, photosynthesis, and essential monoterpene oil(s) yield of Ocimum sanctum. Photosynthetica, 44(3), 474-477. doi: 10.1007/s11099-006-0054-1
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    References

    1. Agrawala, S.C., Sharma, C.P.: The standardization of sand and water culture technique for the study of macro and micronutrients (trace) element deficiencies under Indian conditions.-Curr. Sci. 40: 424-428, 1961.
    2. Ajay, Rathore, V.S.: Effect of Zn2+ stress in rice (Oryza sativa cv. Manhar) on growth and photosynthetic processes.-Photosynthetica 31: 571-584, 1995.
    3. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    4. Aruoma, O.I.: Assesment of potential prooxidant and antioxidant actions.-J. amer. Oil chem. Soc. 73: 1617-1625, 1996. Go to original source...
    5. Chakmak, I., Engels, C.: Role of mineral nutrients in photosynthesis and yield formation.-In: Ringel, Z. (ed.): Mineral Nutrition of Crops. Pp. 141-168. Haworth Press, New York 1999.
    6. Clevenger, J.F.: Apparatus for determination of essential oils.-J. amer. pharmac. Assoc. 17: 346, 1928. Go to original source...
    7. Dat, J., Vandenabeele, S., Vranova, E., Van Montagu, M., Inze, D., Van Bresugen, F.: Dual action of the active oxygen species during plant stress response.-Cell. mol. Life Sci. 57: 779-795, 2000. Go to original source...
    8. Dell, B., Wilson, S.A.: Effect of Zn supply on growth of three species of Eucalyptus seedlings and wheat.-Plant Soil 88: 377-384, 1985. Go to original source...
    9. Douglas, J.S.: Essential oil crops and their uses.-World Crops 21: 49-54, 1969.
    10. Erickson, R.E.: Industrial importance of monoterpenes and essential oils.-Lloydia 29: 8-19, 1976.
    11. Gershenzon, J., Croteau, R.: Regulation of monoterpene biosynthesis in higher plants.-In: Towers, G.H.N., Safford, H.A. (ed.): Biochemistry of the Mevalonoic Acid Pathway to Terpenoids. Pp. 99-159. Plenum Press, New York 1991. Go to original source...
    12. Hewitt, E.J.: Sand and water culture methods used in the study of plant nutrition.-Commonwealth Bureau bot. Plantation Crops tech. Commun. 22: 405-439, 1952.
    13. Hoagland, D.R., Arnon, D.I.: The water culture method for growing plants without soil.-Calif. Agr. Exp. Stat. Circ. 347: 1-32, 1952.
    14. Maffei, M., Codignola, A.: Photosynthesis, photorespiration and herbicide effect on terpene production in peppermint (Mentha piperita L.).-J. essent. Oil Res. 2: 275-286, 1990. Go to original source...
    15. Marschner, H.: Function of mineral nutrients. Micronutrients.-In: Marschner, H. (ed.): Mineral Nutrition of Higher Plants. Pp. 269-300. Academic Press, New York 1986.
    16. Misra, A.: Genotypic variation of manganese toxicity and tolerance of Japanese mint.-J. Herbs Spices medic. Plants 4: 3-13, 1996. Go to original source...
    17. Misra, A., Sharma, S.: Zn concentration for essential oil yield and menthol concentration of Japanese mint.-Fertilizer Res. 29: 261-265, 1991. Go to original source...
    18. Misra, A., Srivastava, N.K.: Effect of the triacontanol formulation Miraculon on photosynthesis, growth, nutrient uptake and essential oil yield of lemon grass (Cymbopogon flexuosus Steud. Watts).-Plant Growth Regul. 10: 57-63, 1991. Go to original source...
    19. Misra, A., Srivastava, N.K., Sharma, S.: Role of an antioxidant on net photosynthetic rate, carbon partitioning and oil accumulation in sweet mint.-Agrotropica 15: 69-74, 2003.
    20. Misra, A., Srivastava, A.K., Srivastava, N.K., Khan, A.: Zn-acquisition and its role in growth, photosynthesis, photosynthetic pigments, and biochemical changes in essential monoterpene oil(s) of Pelargonium graveolens.-Photosynthetica 43: 153-155, 2005. Go to original source...
    21. Neil, S.J., Desikan, R., Clarke, A., Hurst, R.D., Hancock, J.T.: Hydrogen peroxide and nitric oxide as siganalling molecules in plants.-J. exp. Bot. 53: 1643-1650, 2002. Go to original source...
    22. Ohki, K.: Effect of zinc nutrition on photosynthesis and carbonic anhydrase activity in cotton.-Physiol. Plant. 38: 300-304, 1976. Go to original source...
    23. Ohki, K.: Zinc concentration in soybean as related to growth, photosynthesis, and carbonic anhydrase activity.-Crop Sci. 18: 79-82, 1978. Go to original source...
    24. Putievsky, E., Galambqsi, B.: Production systems of sweet basil.-In: Hiltunen, R., Holm, Y. (ed.): Basil the Genus Ocimum. Pp. 167-171. Harwood Academic Publ., New York 1997.
    25. Randall, P.J., Bouma, D.: Zinc deficiency, carbonic anhydrase, and photosynthesis in leaves of spinach.-Plant Physiol. 52: 229-232, 1973. Go to original source...
    26. Richards, K.D., Schott, E.J., Sharma, Y.K., Davis, K.P., Gardener, R.C.: Aluminium induces oxidative stress genes in Arabidopsis thaliana.-Plant Physiol. 116: 409-418, 1998. Go to original source...
    27. Sharon, L.M., Kay, E., Lew, J.Y.: Peroxidase isoenzymes from horse raddish roots 1-1 Isolation and physical properties.-J. biol. Chem. 241: 2166-2172, 1966. Go to original source...
    28. Singh, A.: Chemical and and biochemical aspects of activated oxygen, singlet oxygen, superoxide anion and related species.-In: Mequel, J., Quintanilha, A.T., Weber, H. (ed.): CRC Handbook of Free Radicals and an Anioxidant in Biomedicine. Vol. 1. Pp. 17-28. CRC Press, Boca Raton 1989.
    29. Srivastava, N.K., Misra, A., Sharma, S.: Effect of Zn deficiency on net photosynthetic rate, 14C partitioning, and oil accumulation in leaves of peppermint.-Photosynthetica 33: 71-79, 1997. Go to original source...

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