PHOTOSYNTHETICA

Photosynthetica 2007, 45(1):36-42 | DOI: 10.1007/s11099-007-0006-4

Phenolic acclimation to ultraviolet-A irradiation in Eucalyptus nitens seedlings raised across a nutrient environment gradient

D. C. Close^1,2,3, C. McArthur^1,4, A. E. Hagerman⁵, N. W. Davies⁶, C. L. Beadle^1,7,*

¹ Cooperative Research Centre for Sustainable Production Forestry, Hobart, Australia

² Schools of Zoology, Plant and Agricultural Science, University of Tasmania, Hobart, Australia

³ Science Directorate, Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth, Perth, Australia

⁴ School of Biological Sciences, The University of Sydney, Australia

⁵ Department of Chemistry and Biochemistry, Miami University, Oxford, USA

⁶ Central Science Laboratory, University of Tasmania, Hobart, Australia

⁷ CSIRO ENSIS, Hobart, Australia

We investigated the effects of long-term acclimation of Eucalyptus nitens seedlings to ultraviolet-A (UV-A) irradiation (320-400 nm) on phenolic compounds (gallotannins, stilbenes, and flavonols), photochemical efficiency, and chlorophyll and carotenoid contents. Seedlings were raised under four nutrient regimes, ranging from low to high application rates, in an environment that included or excluded UV-A irradiance. Our aims were: to classify phenolic compounds that absorb in the UV-A and their relative contribution to total UV-A absorption; to identify how phenolic compounds respond to UV-A exposure and exclusion, and to determine how plant nutrient status affects acclimation of photo-and pigment-chemistry to UV-A exposure and exclusion. Gallotannins contributed to only a minor fraction of total absorption within the lower range (320-360 nm) of the UV-A spectrum. Stilbene and flavonol compounds dominated absorption within the 320-360 and 360-400 nm ranges, respectively. Contents of gallotannin were generally high in UV-A-exposed seedlings. Although there was a significant effect of UV-A on contents of stilbenes, a general response (across nutrient treatment comparisons) was not evident. Contents of flavonols were not affected by UV-A exposure. Contents of gallotannin, stilbene, and flavonols decreased from low to high nutrient-application treatments. There were no effects of UV-A on photochemical efficiency or pigment-chemistry.

Additional key words: carotenoids; chlorophyll; flavonol; fluorescence induction; gallotanins; nitrogen; stilbene; xanthophyll cycle

Received: May 15, 2006; Accepted: August 28, 2006; Published: March 1, 2007  Show citation