Photosynthetica 2007, 45(3):419 | DOI: 10.1007/s11099-007-0070-9

Changes in leaf photosynthesis of transgenic rice with silenced OsBP-73 gene

Y. Chen1,2, D. Q. Xu1,2,*
1 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
2 Graduate School of the Chinese Academy of Sciences, China

In comparison with its wild type (WT), the transgenic (TG) rice with silenced OsBP-73 gene had significantly lower plant height, grain number per panicle, and leaf net photosynthetic rate (P N). Also, the TG rice showed significantly lower chlorophyll (Chl), ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO), RuBPCO activase, and RuBP contents, photosystem 2 (PS2) photochemical efficiency (Fv/Fm and ΔF/Fm'), apparent quantum yield of carbon assimilation (Φc), carboxylation efficiency (CE), photosynthetic electron transport and photophosphorylation rates as well as sucrose phosphate synthase activity, but higher intercellular CO2 concentration, sucrose, fructose, and glycerate 3-phosphate contents, and non-photochemical quenching of Chl fluorescence (NPQ). Thus the decreased P N in the TG rice leaves is related to both RuBP carboxylation and RuBP regeneration limitations, and the latter is a predominant limitation to photosynthesis.

Additional key words: carboxylation efficiency; intercellular CO2 concentration; net photosynthetic rate; photochemical efficiency; Oryza; ribulose-1,5-bisphosphate (RuBP); ribulose-1,5-bisphosphate carboxylase, oxygenase (RuBPCO); RNA interference; RuBP regeneration limitation; stomatal conductance

Received: August 17, 2006; Accepted: February 8, 2007; Published: September 1, 2007  Show citation

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Chen, Y., & Xu, D.Q. (2007). Changes in leaf photosynthesis of transgenic rice with silenced OsBP-73 gene. Photosynthetica, 45(3), 419. doi: 10.1007/s11099-007-0070-9
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