Photosynthetica 2007, 45(3):477-480 | DOI: 10.1007/s11099-007-0081-6

Effect of seed soaking with sulphydryl compounds on the photochemical efficiency and antioxidant defence system during the growth of pearl millet under water limiting environment

N. K. Ramaswamy2,*, N. S. Nathawat1, J. S. Nair2, H. R. Sharma1, S. M. Kumawat1, Govind Singh1, M. P. Sahu1, S. F. D'Souza2
1 Rajasthan Agricultural University, Bikaner, India
2 Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India

Pearl millet (Pennisetum glaucum L. cv. HHB-67) seeds were pre-soaked in sulphydryl compounds (dithiothreitol, thioglycollic acid, thiourea, and cysteine). In plants at 59 and 67 d after sowing (DAS), activities of photosystem (PS) 2 (ferricyanide site) and PS1, both chloroplastic and total superoxide dismutase, glutathione reductase, and glutathione-S-transferase increased after all sulphydryl pre-treatments at both stages of plant development. Also dry matter of plant parts sampled at 55 DAS was higher after thiol-treatments in comparison with control.

Additional key words: antioxidant enzymes; drought tolerance; photosystems 1 and 2; seed soaking; thiols

Received: October 16, 2006; Accepted: December 12, 2006; Published: September 1, 2007  Show citation

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Ramaswamy, N.K., Nathawat, N.S., Nair, J.S., Sharma, H.R., Kumawat, S.M., Singh, G., Sahu, M.P., & D'Souza, S.F. (2007). Effect of seed soaking with sulphydryl compounds on the photochemical efficiency and antioxidant defence system during the growth of pearl millet under water limiting environment. Photosynthetica, 45(3), 477-480. doi: 10.1007/s11099-007-0081-6
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    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Becana, M., Aparicio-Tejo, P., Irigoyen, J.J., Sanchez-Diaz, M.: Some enzymes of hydrogen peroxide metabolism in leaves and root nodules of Medicago sativa.-Plant Physiol. 82: 1169-1171, 1986. Go to original source...
    3. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    4. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599-626, 1992. Go to original source...
    5. Giannopolitis, C.N., Ries, S.K.: Superoxide dismutase. I. Occurrence in higher plants.-Plant Physiol. 59: 309-314, 1977. Go to original source...
    6. Gilbert, H.F., McLean, V., McLean, M.: Molecular and cell aspects of thiol-disulphide exchange.-Adv. Enzymol. related mol. Biol. 63: 169-172, 1990. Go to original source...
    7. Izawa, S.: Acceptors and donors for chloroplast electron transport.-Meth. Enzymol. 69: 413-433, 1980. Go to original source...
    8. Izawa, S., Good, N.E. The stoichiometric relation of phosphorylation to electron transport in isolated chloroplasts.-Biochim. biophys. Acta 162: 380-391, 1968. Go to original source...
    9. Jia, W., Zhang, J.: Water stress-induced abscisic acid accumulation in relation to reducing agents and sulfhydryl modifiers in maize plant.-Plant Cell Environ. 23: 1389-1395, 2000. Go to original source...
    10. Kranner, I., Grill, D.: Significance of thiol-disulfide exchange in resting stages of plant development.-Bot. Acta 109: 8-14, 1996. Go to original source...
    11. Mannervik, B., Guthenberg, C.: Glutathione transferase (Human placenta).-Meth. Enzymol. 77: 231-235, 1981. Go to original source...
    12. Nayak, L., Biswal, B., Ramaswamy, N.K., Iyer, R.K., Nair, J.S., Biswal, U.C.: Ultraviolet-A in induced changes in photosystem II of thylakoids: Effects of senescence and high growth temperature.-J. Photochem. Photobiol. B 70: 59-65, 2003. Go to original source...
    13. Parihar, G.N., Sahu, M.P., Joshi, N.L.: Nitrogen, sulphur and thiourea nutrition of pearl millet (Pennisetum glaucum (L.) R. Br.). I. Effect on growth and dry matter production.-Ann. Arid Zone 36: 353-362, 1997.
    14. Parihar, G.N., Sahu, M.P., Joshi, N.L.: Nitrogen, sulphur and thiourea nutrition of pearl millet (Pennisetum glaucum (L.) R. Br.). II. Effect on yield and yield components.-Ann. Arid Zone 37: 59-67, 1998.
    15. Sahu, M.P., Solanki, N.S., Dashora, L.N.: Effect of thiourea, thiamine and ascorbic acid on growth and yield of maize (Zea maize L.).-J. Agron. Crop Sci. 171: 65-69, 1993. Go to original source...
    16. Shaedle, M., Bassham, J.A.: Chloroplast glutathione reductase.-Plant Physiol. 59: 1011-1012, 1977. Go to original source...
    17. Sharma, G.K.: Effect of Soil-Applied Sulphur and Select Chemicals on Dry Matter Partitioning and Grain Production Efficiency of Pearl Millet.-M.Sc.(Ag.) Thesis. Rajasthan College of Agriculture, Udaipur, Rajasthan Agricultural University, Bikaner 1988.
    18. Smirnoff, N.: Antioxidant systems and plants response to the environment.-In: Smirnoff, N. (ed.): Environment and Plant Metabolism. Pp. 217-242. Bios Scientific Publ., Oxford 1995.
    19. Wright, S.T.C., Hiron, R.W.P.: (+)-abcisic acid, the growth inhibitor induced in detached wheat leaves by a period of wilting.-Nature 224: 719-720, 1969. Go to original source...

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