Photosynthetica 2008, 46(1):98-106 | DOI: 10.1007/s11099-008-0017-9

Ultraviolet-B radiation (280-315 nm) invoked antioxidant defence systems in Vigna unguiculata (L.) Walp. and Crotalaria juncea L.

V. Selvakumar1,*
1 Department of Botany, The American College, Madurai, India

A crop legume Vigna unguiculata L. (Walp.) and a wild legume Crotalaria juncea L. were evaluated for their relative responses to the oxidative stress injury induced by various doses of UV-B radiation (UV-B, 280-315 nm; 0, 1.0, 1.4, 4.7, and 6.0 kJ m-2 d-1). A dose-dependent damage in lipid peroxidation was determined as an index of membrane injury caused by UV-B. The impact was significantly higher in V. unguiculata than in C. juncea. The specific activities of superoxide dismutase, ascorbate peroxidase, monodehydroascorbate reductase, and dehydroascorbate reductase increased directly proportional to UV-B doses. However, the activities of these enzymes were significantly higher in V. unguiculata than in C. juncea indicating that V. unguiculata was inflicted with more severe oxidative stress injury under UV-B. In C. juncea the glutathione reductase and ascorbate oxidase activities were 35 and 40 % greater than in V. unguiculata, respectively. Further, the non-enzymatic antioxidants ascorbate and glutathione, and their reduced/oxidizes ratios in C. juncea were much greater than V. unguiculata indicating C. juncea has an inherently greater antioxidative potential than V. unguiculata. Thus C. juncea is better adapted to oxidative stress than V. unguiculata by means of efficient cellular antioxidant mechanisms helping to combat the photooxidative stress injury elicited by UV-B.

Additional key words: ascorbate-glutathione cycle; ascorbate oxidase and peroxidase; catalase; dehydroascorbate reductase; glutathione transferase; monodehydroascorbate reductase; oxidative stress; peroxidase; superoxide dismutase

Received: March 6, 2007; Accepted: September 24, 2007; Published: March 1, 2008  Show citation

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Selvakumar, V. (2008). Ultraviolet-B radiation (280-315 nm) invoked antioxidant defence systems in Vigna unguiculata (L.) Walp. and Crotalaria juncea L. Photosynthetica, 46(1), 98-106. doi: 10.1007/s11099-008-0017-9
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