Photosynthetica 2008, 46(3):370-377 | DOI: 10.1007/s11099-008-0067-z

Developmental phase-dependent photosynthetic responses to ultraviolet-B radiation: damage, defence, and adaptation of primary leaves of wheat seedlings

M. K. Pradhan1, L. Nayak1, P. N. Joshi1,*, P. K. Mohapatra2, L. Patro2, B. Biswal2, U. C. Biswal2
1 Laboratory of Biophysics and Biochemistry, Anchal College, Orissa, India
2 Laboratory of Biophysics and Biochemistry, School of Life Sciences, Sambalpur University, Orissa, India

Alterations in photosynthetic capacity of primary leaves of wheat seedlings in response to ultraviolet-B (UV-B; 280-320 nm; 60 µmol m-2 s-1) exposure alone and in combination with photosynthetically active radiation (PAR; 400-800 nm; 200 µmol m-2 s-1) during different phases of leaf growth and development were assessed. UV-B exposure resulted in a phase-dependent differential loss in photosynthetic pigments, photochemical potential, photosystem 2 (PS2) quantum yield, and in vivo O2 evolution. UV-B exposure induced maximum damage to the photosynthetic apparatus during senescence phase of development. The damages were partially alleviated when UV-B exposure was accompanied by PAR. UV-B induced an enhancement in accumulation of flavonoids during all phases of development while it caused a decline in anthocyanin content during senescence. The differential changes in these parameters demonstrated the adaptation ability of leaves to UV-B stress during all phases of development and the ability was modified in UV-B+ PAR exposed samples.

Additional key words: anthocyanin; carotenoids; chlorophyll; flavonoids; O2 evolution; photosynthetically active radiation; phases of leaf development; senescence; thylakoid membrane

Received: November 20, 2007; Accepted: March 14, 2008; Published: September 1, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Pradhan, M.K., Nayak, L., Joshi, P.N., Mohapatra, P.K., Patro, L., Biswal, B., & Biswal, U.C. (2008). Developmental phase-dependent photosynthetic responses to ultraviolet-B radiation: damage, defence, and adaptation of primary leaves of wheat seedlings. Photosynthetica, 46(3), 370-377. doi: 10.1007/s11099-008-0067-z
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Adamse, P., Britz, S.J.: Amelioration of UV-B damage under high irradiance. I. Role of photosynthesis.-Photochem. Photobiol. 56: 645-650, 1992. Go to original source...
    2. A.-H.-Mackerness, S., Surplus, S.L., Jordan, B.R., Thomas, B.: Effects of supplementary ultraviolet-B radiation on photosynthetic transcripts at different stages of leaf development and light levels in pea (Pisum sativum L.): Role of active oxygen species and antioxidant enzymes.-Photochem. Photobiol. 68: 88-96, 1998.
    3. Babu, T.S., Jansen, M.A.K., Greenberg, B.M., Gaba, V., Malkin, V., Matto, A., Edelman, M.: Amplified degradation of photosystem II D1 and D2 proteins under a mixture of photosynthetically active radiation and UVB radiation: Dependence on redox status of photosystem II.-Photochem. Photobiol. 69: 553-559, 1999. Go to original source...
    4. Beggs, J.C., Wellmann, E.: Photocontrol of flavonoid biosynthesis.-In: Kendrick, R.E., Kronenberg, G.H.M. (ed.): Photomorphogenesis in Plants. Pp. 733-751. Kluwer Academic Publ., Dordrecht 1994. Go to original source...
    5. Bergo, E., Segalla, A., Giacometti, G.M., Tarantino, D., Soave, C., Andreucci, F., Barbato, R.: Role of visible light in the recovery of photosystem II structure and function from ultraviolet-B stress in higher plants.-J. exp. Bot. 54: 1665-1673, 2003. Go to original source...
    6. Biswal, U.C., Biswal, B., Raval, M.K.: Chloroplasts Biogenesis: From Proplastid to Gerentoplasts.-Springer-Verlag, Dordrecht 2003. Go to original source...
    7. Bornman, J.F.: Target sites of UV-B radiation in photosynthesis of higher plants.-J. Photochem. Photobiol. B 4: 145-158, 1989. Go to original source...
    8. Brandt, K., Giannini, A., Lercari, B.: Photomorphogenic responses to UV radiation. III. A comparative study of UV-B effects on anthocyanin and flavonoid accumulation in wild-type and aurea mutant of tomato (Lycopersicon esculentum Mill.).-Photochem. Photobiol. 62: 1081-1087, 1995. Go to original source...
    9. Brosché, M., Strid, A.: Molecular events following perception of ultraviolet-B radiation by plants.-Physiol. Plant. 117: 1-10, 2003. Go to original source...
    10. Caldwell, M.M., Bornman, J.F., Ballare, C.L., Flint, S.D., Kulandaivelu, G.: Terrestrial ecosystems increased solar ultraviolet radiation and interaction with other climatic change factors.-Photochem. photobiol. Sci. 7: 252-266, 2007. Go to original source...
    11. Dillenburg, L.R., Sullivan, J.H., Teramura, A.H.: Leaf expansion and development of photosynthetic capacity and pigments in Liquidamar styraciflua (Hamamelidaceae). Effects of UV-B radiation.-Amer. J. Bot. 82: 878-885, 1995. Go to original source...
    12. Flint, S.D., Jordan, P.W., Caldwell, M.M.: Plant protective response to enhanced UV-B radiation under field conditions: leaf optical properties and photosynthesis.-Photochem. Photobiol. 41: 95-99, 1985. Go to original source...
    13. Gao, W., Zheng, Y., Slasser J.R., Heider, G.M., Grant, R.H., Xu, J., He, D.: Effect of supplementary UV-B irradiance on maize yield and qualities: a field experiment.-Photochem. Photobiol. 80: 127-131, 2004. Go to original source...
    14. Glantz, S.A.: Primer of Biostatistics. 2nd Ed.-McGraw Hill, New York 1989.
    15. Govindjee: Sixty-three years since Kautsky: Chlorophyll a fluorescence.-Aust. J. Plant Physiol. 22: 131-160, 1995. Go to original source...
    16. Hada, M., Hino, K., Takeuchi, Y.: Development of UV defense mechanisms during growth of spinach seedlings.-Plant Cell Physiol. 42: 784-787, 2001. Go to original source...
    17. Holton, T.A., Cornish, E.C.: Genetics and biochemistry of anthocyanin biosynthesis.-Plant Cell 7: 1071-1083, 1995. Go to original source...
    18. Izawa, S., Good, N.E.: The stoichiometric relation of phosphorylation to electron transport in isolated chloroplasts.-Biochim. biophys. Acta 162: 380-391, 1968. Go to original source...
    19. Jansen, M.A.K., Gaba, V., Greenberg, B.M.: Higher plants and UV-B radiation: balancing damage, repair and acclimation.-Trends Plant Sci. 39: 131-135, 1998. Go to original source...
    20. Jordan, B.R.: Molecular response of plant cells to UV-B stress.-Funct. Plant Biol. 29: 909-918, 2002. Go to original source...
    21. Jordan, B.R., James, P.E., Strid, Å., Anthony, R.G.: The effect of ultraviolet-B radiation on gene expression and pigment composition in etiolated and green pea leaf tissue: UV-B induced changes are gene-specific and dependent upon the developmental stage.-Plant Cell Environ. 17: 45-54, 1994. Go to original source...
    22. Joshi, P.N., Ramaswamy, N.K., Raval, M.K., Desai, T.S., Nair, P.M., Biswal, U.C.: Alteration in photosystem II photochemistry of thylakoids isolated from senescing leaves of wheat seedlings.-J. Photochem. Photobiol. B 20: 197-202, 1993. Go to original source...
    23. Kolb, C.A., Kaser, M.A., Kopecky, J., Zotz, G., Riederer, M., Pfundel, E.E.: Effects of natural intensities of visible and ultraviolet radiation on epidermal ultraviolet screening and photosynthesis in grape leaves.-Plant Physiol. 127: 863-875, 2001. Go to original source...
    24. Lau, T.S.L., Eno, E., Goldstein, G., Smith, C., Christopher, D.A.: Ambient levels of UV-B in Hawaii combined with nutrient deficiency decrease photosynthesis in near-isogenic maize lines varying in leaf flavonoids: Flavonoids decrease photoinhibition in plants exposed to UV-B.-Photosynthetica 44: 394-403, 2006. Go to original source...
    25. Lichtenthaler, H.K.: In vivo chlorophyll fluorescence as a tool for stress detection in plants.-In: Lichtenthaler, H.K. (ed.): Applications of Chlorophyll Fluorescence. Pp. 129-142. Kluwer Academic Publ., Dordrecht-Boston-London 1988. Go to original source...
    26. Lichtenthaler, H.K.: Applications of chlorophyll fluorescence in stress physiology and remote sensing.-In: Steven, M.D., Clark, J.A. (ed.): Applications of Remote Sensing in Agriculture. Pp. 287-305. Butterworth Scientific, London 1990. Go to original source...
    27. Lindoo, S.J., Caldwell, M.M.: Ultraviolet-B radiation-induced inhibition of leaf expansion and promotion of anthocyanin production.-Plant Physiol. 61: 278-282, 1978. Go to original source...
    28. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
    29. Mohr, H., Schopfer, P.: Photosynthesis as a chloroplasts function.-In: Mohr, H., Schopfer, P. (ed.): Plant Physiology. Pp. 149-185. Springer-Verlag, Berlin-Heidelberg-New York 1995. Go to original source...
    30. Olsson, L.C., Veit, M., Weissenböck, G., Bornman, J.F.: Differential flavonoid response to enhanced UV-B radiation in Brassica napus.-Phytochemistry 49: 1021-1028, 1998. Go to original source...
    31. Panda, S., Mishra, A.K., Biswal, U.C.: Manganese induced peroxidation of thylakoid lipids and changes in chlorophyll fluorescence during aging of cell free chloroplasts in light.-Phytochemistry 26: 3217-3219, 1987. Go to original source...
    32. Pradhan, M.K., Joshi, P.N., Nair, J.S., Ramaswamy, N.K., Iyer, R.K., Biswal, B., Biswal, U.C.: UV-B exposure enhances senescence of wheat leaves: modulation by photosynthetically active radiation.-Radiat. environ. Biophys. 45: 221-229, 2006. Go to original source...
    33. Pfündel, E.E., Pan, R.-S., Dilley, R.A.: Inhibition of violaxanthin deepoxidation by ultraviolet-B radiation in isolated chloroplasts and intact leaves.-Plant Physiol. 98: 1372-1380, 1992. Go to original source...
    34. Rajagopal, S., Sicora, C., Várkonyi, Z., Mustárdy, L., Mohanty P.: Protective effect of supplemental low intensity white light on ultraviolet-B exposure-induced impairment in cyanobacterium Spirulina platensis: formation of air vacuoles as a possible protective measure.-Photosynth. Res. 85: 181-189, 2005. Go to original source...
    35. Rodrigues, G.C., Jansen, M.A.K., van den Noort, M.E., van Reansen, J.J.S.: Evidences of semireduced primary quinone electron acceptor of photosystem II being a photosensitizer for UV-B damage to photosynthetic apparatus.-Plant Sci. 170: 283-290, 2006. Go to original source...
    36. Rousseaux, M.C., Ballaré, C.L., Giordano, C.V., Scopel, A.L., Zima, A.M., Szwarcberg-Bracchitta, M., Searles, P.S., Caldwell, M.M., Díaz, S.B.: Ozone depletion and UVB radiation: Impact on plant DNA damage in southern South America.-Proc. nat. Acad. Sci. USA 96: 15310-15315, 1999. Go to original source...
    37. Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer.-Photosynth. Res. 10: 51-62, 1986. Go to original source...
    38. Stapleton, A.E., Walbot, V.: Flavonoids can protect maize DNA from the induction of ultraviolet radiation damage.-Plant Physiol. 105: 881-889, 1994. Go to original source...
    39. Strasser, R.J., Tsimilli-Michael, M., Srivastava, A.: Analysis of chlorophyll a transient.-In: Papageorgiou, G., Govindjee (ed.): Chlorophyll a Fluorescence: A Signature of Photosynthesis. Pp. 321-362. Springer, Dordrecht 2004. Go to original source...
    40. Strid, Å., Anderson, J.M.: UV-B damage and protection at the molecular level in plants.-Photosynth. Res. 39: 475-489, 1994. Go to original source...
    41. Strid, Å., Chow, W.S., Anderson, J.M.: Effects of supplementary ultraviolet-B radiation on photosynthesis in Pisum sativum.-Biochim. biophys. Acta 1020: 260-268, 1990. Go to original source...
    42. Teramura, A.H., Caldwell, M.M.: Effects of ultraviolet-B irradiances on soybean. IV. Leaf ontogeny as a factor in evaluating ultraviolet-B irradiance effects on net photosynthesis.-Amer. J. Bot. 68: 934-941, 1981. Go to original source...
    43. van Kooten, O., Snel, J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology.-Photosynth. Res. 25: 147-150, 1990. Go to original source...
    44. van Rensen, J.J.S., Vredenberg, W.J., Rodrigues, G.C.: Time sequence of the damage to the acceptor and donor sides of photosystem II by UV-B radiation as evaluated by chlorophyll a fluorescence.-Photosynth. Res. 94: 291-297, 2007. Go to original source...
    45. Vass, I.: Adverse effects of UV-B light on the structure and function of the photosynthetic apparatus.-In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 931-949. Marcel Dekker, New York-Basel-Hong Kong 1997.
    46. Vass, I., Szilárd, A., Sicora, C.: Adverse effects of UV-B light on the structure and function of the photosynthetic apparatus.-In: Pessarakli, M. (ed.): Handbook of Photosynthesis. 2nd Ed. Pp. 827-833. Taylor & Francis, Boca Raton-London-New York-Singapore 2005.
    47. Wellburn, A.R., Lichtenthaler, H.K.: Formulae and program to determine total carotenoids and chlorophylls a and b of leaf extracts in different solvents.-In: Sybesma, C. (ed.): Advances in Photosynthesis Research. Vol. II. Pp. 9-12. Martinus Nijhoff/Dr W. Junk Publ., The Hague-Boston-Lancaster 1984. Go to original source...
    48. Wilson, M.I., Greenberg, B.M.: Protection of the D1 photosystem II reaction center protein from degradation in ultraviolet radiation following adaptation of Brasicca napus L. to growth in ultraviolet-B.-Photochem. Photobiol. 57: 556-563, 1993. Go to original source...
    49. Xiong, F.S., Day, D.A.: Effect of solar ultraviolet-B radiation during springtime ozone depletion on photosynthesis and biomass production of Antarctic vascular plant.-Plant Physiol. 125: 738-751, 2001. Go to original source...
    50. Zuk-Golaszewska, K., Upadhyaya, M.K., Golaszewski, J.: The effect of UV-B radiation on plant growth and development.-Plant Soil Environ. 49: 135-140, 2003. Go to original source...

    Return to the content