Photosynthetica 2008, 46(4):525-530 | DOI: 10.1007/s11099-008-0089-6

Isolation and characterization of photosystem 2 PsbR gene and its promoter from drought-tolerant plant Prosopis juliflora

G. Suja1, A. Parida1,*
1 Department of Plant Molecular Biology, M.S. Swaminathan Research Foundation, Institutional Area, Taramani, Chennai, India

Prosopis juliflora is a hardy plant tolerant to drought, salinity, extremes of soil pH, and heavy metal stress. We isolated and characterized a photosystem 2 (PS2) gene PsbR (Pj PsbR) and its promoter. Northern analysis for Pj PsbR in P. juliflora leaves under 25 % polyethylene glycol stress showed steady decrease in transcript level at 12, 24, and 48 h after stress application. Under 90 mM H2O2 stress, transcript level dropped drastically at 12 h, but increased again compared to the control at 24 h. A 1.7 kb fragment upstream the 5' UTR of this gene (putative promoter) was isolated and analyzed in silico. Several putative cis-acting DNA elements were identified in this sequence.

Additional key words: H2O2; Northern analysis; photosystem 2; polyethylene glycol; proteins; transcript level; water deficit

Received: February 20, 2008; Accepted: July 29, 2008; Published: December 1, 2008  Show citation

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Suja, G., & Parida, A. (2008). Isolation and characterization of photosystem 2 PsbR gene and its promoter from drought-tolerant plant Prosopis juliflora. Photosynthetica, 46(4), 525-530. doi: 10.1007/s11099-008-0089-6
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