Photosynthetica 2009, 47(3):422 | DOI: 10.1007/s11099-009-0065-9

Short-term dynamics of stomatal response to sudden increase in CO2 concentration in maize supplied with different amounts of water

J. Hladnik1,*, K. Eler1, K. Kržan1, M. Pintar1, D. Vodnik1
1 Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

Environmental factors that influence stomatal conductance (g s) interact through a complex network of signal transduction and have therefore highly interdependent effect.
In the present study we examined how plant water status affects stomatal sensitivity to the change of CO2 concentration ([CO2]). We investigated the short-term dynamic of stomatal response to a sudden [CO2] increase (from 400 to 700 µmol(CO2) mol-1) in maize supplied with different amounts of water (resulting ψw = -0.35, -0.52 and -0.75 MPa). Gas exchange measurements were performed in short logging intervals and the response was monitored under two different levels of water vapour pressure deficit (VPD) of 1 and 2 kPa in order to observe the impact of air humidity. Generalized logistic curves were fitted to standardized stomatal response data, which enabled us to objectively estimate the level (relative decrease of g s) and the dynamics of the response.
Soil water stress and high VPD significantly decreased relative stomatal closure in response to [CO2] rise, but simultaneously accelerated stomatal response to [CO2], as revealed by shorter half life (t1/2). VPD significantly affected the response of well-watered plants. In contrast, a fast stomatal reaction of water-deprived plants was predetermined by a low xylem water potential (ψw) of the leaf and the influence of air humidity was minor.

Additional key words: curve fitting; elevated CO2; stomatal dynamics; water potential; water vapour pressure deficit; Zea mays

Received: April 7, 2009; Accepted: August 17, 2009; Published: September 1, 2009  Show citation

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Hladnik, J., Eler, K., Kržan, K., Pintar, M., & Vodnik, D. (2009). Short-term dynamics of stomatal response to sudden increase in CO2 concentration in maize supplied with different amounts of water. Photosynthetica, 47(3), 422. doi: 10.1007/s11099-009-0065-9
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    References

    1. Ainsworth, E.A., Rogers, A.: The response of photosynthesis and stomatal conductance to rising [CO2]: mechanisms and environmental interactions. - Plant Cell Environ. 30:258-270, 2007. Go to original source...
    2. Assmann, S.M., Grantz, D.A.: The magnitude of the stomatal response to blue-light - modulation by atmospheric humidity. - Plant Physiol. 93: 701-709, 1990. Go to original source...
    3. Barradas, V.L., Jones, H.G., Clark, J.A.: Stomatal responses to changing irradiance in Phaseolus vulgaris L. - J. Exp. Bot. 45: 931-936, 1994. Go to original source...
    4. Barrs, H.D.: Cyclic variations in stomatal aperture, transpiration and leaf water potential under constant environmental conditions. - Annu. Rev. Plant Physiol. 22: 223-236, 1971. Go to original source...
    5. Brearley, J., Venis, M.A., Blatt, M.R.: The effect of elevated CO2 concentrations on K+ and anion channels of Vicia faba L. guard cells. - Planta 203: 145-154, 1997. Go to original source...
    6. Buckley, T.N., Mott, K.A., Farquhar, G.D.: A hydromechanical and biochemical model of stomatal conductance. - Plant Cell Environ. 26: 1767-1785, 2003. Go to original source...
    7. Bunce, J.A.: Does transpiration control stomatal responses to water vapour pressure deficit? - Plant Cell Environ. 20: 131-135, 1997. Go to original source...
    8. Cousins, A.B., Baroli I., Badger, M.R., Ivakov, A., Lea, P.J., Leegood, R.C., von Caemmerer, S.: The role of phosphoenolpyruvate carboxylase during C4 photosynthetic isotope exchange and stomatal conductance. - Plant Physiol. 145: 1006-1017, 2007. Go to original source...
    9. de Araujo, A.C., Kruijt, B., Nobre, A.D., Dolman, A.J., Waterloo, M.J., Moors, E.J., de Souza, J.S.: Nocturnal accumulation of CO2 underneath a tropical forest canopy along a topographical gradient. - Ecol. Appl. 18: 1406-1419, 2008. Go to original source...
    10. Del Pozo, A., Perez, P., Morcuende, R., Alonso, A., Martinez-Carrasco, R.: Acclimatory responses of stomatal conductance and photosynthesis to elevated CO2 and temperature in wheat crops grown at varying levels of N supply in a Mediterranean environment. - Plant Sci. 169: 908-916, 2005. Go to original source...
    11. Dzikiti, S., Steppe, K., Lemeur, R., Milford, J.R.: Whole-tree level water balance and its implications on stomatal oscillations in orange trees [Citrus sinensis (L.) Osbeck] under natural climatic conditions. - J. exp. Bot. 58: 1893-1901, 2007. Go to original source...
    12. Frechilla, S., Talbott, L.D., Zeiger, E.: The CO2 response of Vicia guard cells acclimates to growth environment. - J. exp Bot. 53: 545-550, 2002. Go to original source...
    13. Hanstein, S.M., Felle, H.H.: CO2-triggered chloride release from guard cells in intact fava bean leaves. Kinetics of the onset of stomatal closure. - Plant Physiol. 130: 940-950, 2002. Go to original source...
    14. Herppich, W.B., von Willert, D.J.: Dynamic changes in leaf bulk water relations during stomatal oscillations in mangrove species - continuous analysis using a dewpoint hygrometer. - Physiol. Plant. 49, 479-485: 1995. Go to original source...
    15. Huxman, T.E., Monson, R.K.: Stomatal responses of C-3, C-3-C-4 and C-4 Flaveria species to light and intercellular CO2 concentration: implications for the evolution of stomatal behaviour. - Plant Cell Environ. 26: 313-322: 2003. Go to original source...
    16. Kaiser, H., Kappen, L.: Stomatal oscillations at small apertures: indications for a fundamental insufficiency of stomatal feedback-control inherent in the stomatal turgor mechanism. - J. exp. Bot. 52: 1303-1313, 2001. Go to original source...
    17. Kamakura, M., Furukawa, A.: Responses of individual stomata in Ipomoea pes-caprae to various CO2 concentrations. - Phys. Plantarum 132: 255-261, 2008. Go to original source...
    18. Klute, A.: Water retention: Laboratory Methods. - In: Klute, A. (ed.): Methods of Soil Analysis. Part 1. 2nd Ed. Pp. 635-662. American Society of Agronomy, Madison 1986. Go to original source...
    19. Lawson, T., Lefebvre, S., Baker, N.R., Morison, J.I.L., Raines, C.A.: Reductions in mesophyll and guard cell photosynthesis impact on the control of stomatal responses to light and CO2. - J. Exp. Bot. 59: 3609-3619, 2008. Go to original source...
    20. Leymarie, J., Lasceve, G., Vavasseur, A.: Interaction of stomatal responses to ABA and CO2 in Arabidiposis thaliana. - Aust. J. Plant Physiol. 25: 785-791, 1998. Go to original source...
    21. Li, S., Assmann, S.M., Albert, R.: Predicting essential components of signal transduction networks: A dynamic model of guard cell abscisic acid signaling. - PLoS Biol. 4: 1732-1748, 2006. Go to original source...
    22. Maherali, H., Johnson, H.B., Jackson, R.B.: Stomatal sensitivity to vapour pressure difference over a subambient to elevated CO2 gradient in a C-3/C-4 grassland. - Plant Cell Environ. 26: 1297-1306, 2003. Go to original source...
    23. Morison, J.I.L.: Intercellular CO2 concentration and stomatal response to CO2. - In: Zeiger, E., Farquhar, G.D., Cowan, I.R. (ed.): Stomatal Function. Pp. 229-251. Stanford University Press, Stanford 1987.
    24. Mott, K.A.: Do stomata respond to CO2 concentrations other than intercellular? - Plant Physiol. 86: 200-203, 1988. Go to original source...
    25. Mott, K.A.: Sensing of atmospheric CO2 by plants. - Plant Cell Environ. 13: 731-737, 1990. Go to original source...
    26. Motulsky, H., Christopoulos, A.: Fitting Models to Biological Data Using Linear and Nonlinear Regression: A Practical Guide to Curve Fitting. - Oxford Univ. Press, Cary 2004.
    27. Pepin, S., Livingston, N.J.: Rates of stomatal opening in conifer seedlings in relation to air temperature and daily carbon gain. - Plant Cell Environ. 20: 1462-1472, 1997. Go to original source...
    28. Pfanz, H., Vodnik, D., Wittmann, C., Aschan, G., Raschi, A.: Plants and geothermal CO2 exhalations. Survival in and adaptation to a high CO2 environment. - In: Esser, K., Lüttge, U., Kadreit, J.W., Beyschlag, W. (ed.): Progress in Botany 65. Pp. 499-538. Springer-Verlag, Berlin - Heidelberg 2004. Go to original source...
    29. Powles, J.E., Buckley, T.N., Nicotra, A.B., Farquhar, G.D.: Dynamics of stomatal water relations following leaf excision. - Plant Cell Environ. 29: 981-992, 2006. Go to original source...
    30. Raschke, K.: Simultaneous requirement of carbon dioxide and abscisic acid for stomatal closing in Xanthium strumarium L. - Planta 125: 243-259, 1975 Go to original source...
    31. Raschke, K., Hanebuth, W.F., Farquhar, G.D.: Relationship between stomatal conductance and light intensity in leaves of Zea mays L. derived from experiments using the mesophyll as shade. - Planta 139: 73-77, 1978. Go to original source...
    32. Šantrůček, J., Sage, R.F.: Acclimation of stomatal conductance to a CO2-enriched atmosphere and elevated temperature in Chenopodium album. - Aust. J. Plant Physiol. 23: 467-478, 1996. Go to original source...
    33. Scholander, P.F., Hemmingsen, E.A., Hammel, H.T., Bradstreet, E.D.P.: Hydrostatic pressure + osmotic potential in leaves of mangroves + some other plants. - Proc. Nat. Acad. Sci. 52: 119-125, 1964. Go to original source...
    34. Schroeder, J.I., Allen, G.J., Hugouvieux, V., Kwak, J.M., Waner, D.: Guard cell signal transduction. - Annu. Rev. Plant Physiol. 52, 627-658, 2001. Go to original source...
    35. Sharkey, T.D., Raschke, K.: Separation and measurement of direct and indirect effects of light on stomata. - Plant Physiol. 68: 33-40, 1981. Go to original source...
    36. Steppe, K., Dzikiti, S., Lemeur, R., Milford, J. R.: Stomatal oscillations in orange trees under natural climatic conditions. - Ann. Bot. 97: 831-835, 2006. Go to original source...
    37. Talbott, L.D., Rahveh, E., Zeiger, E.: Relative humidity is a key factor in the acclimation of the stomatal response to CO2. - J. Exp. Bot. 54: 2141-2147, 2003. Go to original source...
    38. Vavasseur, A., Raghavendra, A.S.: Guard cell metabolism and CO2 sensing. - New Phytol. 165: 665-682, 2005. Go to original source...
    39. West, J.D., Peak, D., Peterson, J.Q., Mott, K.A.: Dynamics of stomatal patches for a single surface of Xanthium strumarium L. leaves observed with fluorescence and thermal images. - Plant Cell Environ. 28: 633-641, 2005. Go to original source...
    40. Ye, Z.-P., Yu, Q.: A coupled model of stomatal conductance and photosynthesis for winter wheat. - Photosynthetica 46: 637-640, 2008. Go to original source...
    41. Young, J.J., Mehta, S., Israelsson, M., Godoski, J., Grill, E., Schroeder, J.I.: CO2 signalling in guard cells: Calcium sensitivity response modulation, a Ca2+-independent phase, and CO2 insensitivity of the gca2 mutant. - Proc. Nat. Acad. Sci. 103: 7506-7511, 2006. Go to original source...
    42. Yu, Q., Zhang, Y.G., Liu, Y.F., Shi, P.L.: Simulation of the stomatal conductance of winter wheat in response to light, temperature and CO2 changes. - Ann. Bot. 93: 435-441, 2004. Go to original source...
    43. Zhu, J., Talbott, L.D., Jin, X., Zeiger, E.: The stomatal response to CO2 is linked to changes in guard cell zeaxanthin. - Plant Cell Environ. 21: 813-820, 1998. Go to original source...

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