Photosynthetica 2009, 47(4):638-640 | DOI: 10.1007/s11099-009-0093-5

Evaluation of light-harvesting complex proteins as senescence-related protein markers in detached rice leaves

K. Kang1, Y. S. Kim1, S. Park1, K. Back1,*
1 Department of Biotechnology, Interdisciplinary Program for Bioenergy and Biomaterials of Graduate Program, Biotechnology Research Institute, Chonnam National University, Gwangju, South Korea

Ten light-harvesting complex (Lhc) proteins were investigated to determine which was the most appropriate protein marker of senescence in detached rice leaves. The levels of Lhc proteins were monitored by immunoblot analysis, which was conducted using commercially available antibodies raised against each Lhc protein. Among the Lhc proteins evaluated in this study, Lhca1, Lhcb1, Lhcb2, Lhcb3, and Lhcb5 were not appropriate to be used as senescence markers while others can be used after optimization of the procedure.

Additional key words: abscisic acid; Oryza sativa; pigment-binding proteins; senescence-associated genes; zeatin

Received: April 7, 2009; Accepted: December 8, 2009; Published: December 1, 2009  Show citation

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Kang, K., Kim, Y.S., Park, S., & Back, K. (2009). Evaluation of light-harvesting complex proteins as senescence-related protein markers in detached rice leaves. Photosynthetica, 47(4), 638-640. doi: 10.1007/s11099-009-0093-5
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