Photosynthetica 2013, 51(3):387-394 | DOI: 10.1007/s11099-013-0035-0

Effects of ectopically expressed hyperthermophilic archaeon (Pyrococcus furiosus) ribulose-1,5-bisphosphate carboxylase/oxygenase on tobacco photosynthesis

X. G. Li1,*, J. Yang2, R. Wang2, X. F. Tang3, J. J. Meng1, H. J. Qin4, X. Liu4, F. Guo1, S. B. Wan1,*
1 High-Tech Research Center, Shandong Academy of Agricultural Sciences and Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Ji'nan, China
2 Zhengzhou Tobacco Research Institute of China, National Tobacco Corporation, Zhengzhou, China
3 College of Life Sciences, Wuhan University, Wuhan, China
4 The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China

Pyrococcus furiosus is a hyperthermophilic archaeon. Its ribulose-1,5-bisphosphate carboxylase/oxygenase (PfRubisco) has only large subunit (L). PfRubisco has a novel (L2)5, decameric structure and it possesses higher carboxylase activity and thermotolerance. To assess the potential functionality of PfRubisco in higher plants under high-temperature stress, PfRubisco coding sequence was transiently expressed in Nicotiana benthamiana by Pea early browning virus mediated ectopic expression. The transgenic PfRubisco plants produced chlorotic yellow stripes in their leaves. Relative to the control leaves, those with yellow stripes exhibited decreased net photosynthetic rate and chlorophyll content, altered chloroplast ultrastructure, and more severe photoinhibition of both photosystem I and II. We concluded that the ectopic expression of PfRubisco might disrupt the chloroplast development and function in N. benthamiana. The potential cause of the disruption was discussed.

Additional key words: high-temperature stress; Nicotiana benthamiana; photosynthesis; Rubisco; virus mediated ectopic expression

Received: May 31, 2012; Accepted: January 17, 2013; Published: September 1, 2013  Show citation

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Li, X.G., Yang, J., Wang, R., Tang, X.F., Meng, J.J., Qin, H.J., ... Wan, S.B. (2013). Effects of ectopically expressed hyperthermophilic archaeon (Pyrococcus furiosus) ribulose-1,5-bisphosphate carboxylase/oxygenase on tobacco photosynthesis. Photosynthetica, 51(3), 387-394. doi: 10.1007/s11099-013-0035-0
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