Photosynthetica 2014, 52(2):203-210 | DOI: 10.1007/s11099-014-0019-8

Accumulation of photosynthetic pigments in Larix decidua Mill. and Picea abies (L.) Karst. cotyledons treated with 5-aminolevulinic acid under different irradiation

N. Maximová1,*, Ľ. Slováková2
1 Institute of High Mountain Biology, University of Žilina, Tatranská Javorina 7, Slovak Republic
2 Department of Plant Physiology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic

European larch (Larix decidua Mill.) and Norway spruce [Picea abies (L.) Karst.] synthesize chlorophyll (Chl) in darkness. This paper compares Chl accumulation in 14-d-old dark-grown seedlings of L. decidua and P. abies after shortterm (24 h) feeding with 5-aminolevulinic acid (ALA). We used two ALA concentrations (1 and 10 mM) fed to cotyledons of both species in darkness and in continuous light. The dark-grown seedlings of L. decidua accumulated Chl only in trace amounts and the seedlings remained etiolated. In contrast, P. abies seedlings grown in darkness were green and had significantly higher Chl content. After ALA feeding, higher protochlorophyllide (Pchlide) content was observed in L. decidua than in P. abies cotyledons incubated in darkness. Although short-term ALA feeding stimulated the synthesis of Pchlide, Chl content did not change significantly in cotyledons incubated in darkness. The Chl accumulation in cotyledons fed with ALA was similar to the rate of Chl accumulation in the controls. Higher Chl accumulation was reported in control samples after illumination: 86.9% in L. decidua cotyledons and 46.4% in P. abies cotyledons. The Chl content decreased and bleaching occurred in cotyledons incubated with ALA in light due to photooxidation. Analyses of Chlbinding proteins (D1 and LHCIIb) by Western blotting proved differences between Chl biosynthesis in L. decidua and P. abies seedlings in the dark and in the light. No remarkable increase was found in protein accumulation (D1 and LHCIIb) after ALA application. Our results showed interspecific difference in Chl synthesis between two gymnosperms. Shortterm ALA feeding did not stimulate Chl synthesis, thus ALA synthesis was not the rate-limiting step in Chl synthesis in the dark.

Additional key words: chlorophyll biosynthesis; D1 protein; light-harvesting complex; protochlorophyllide

Received: December 17, 2012; Accepted: August 20, 2013; Published: June 1, 2014  Show citation

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Maximová, N., & Slováková, Ľ. (2014). Accumulation of photosynthetic pigments in Larix decidua Mill. and Picea abies (L.) Karst. cotyledons treated with 5-aminolevulinic acid under different irradiation. Photosynthetica, 52(2), 203-210. doi: 10.1007/s11099-014-0019-8
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    References

    1. Al-Thabet, S.S.: Promotive effect of 5-amino levulinic acid on growth and yield of wheat grown under dry conditions. - J. Agron. 5: 45-49, 2006. Go to original source...
    2. Alosi, M.C., Neale, D.B., Kinlaw, C.S.: Expression of cab genes in Douglas-fir is not strongly regulated by light. - Plant Physiol. 93: 829-832, 1990. Go to original source...
    3. Armstrong, G.A.: Greening in the dark: light-independent chlorophyll biosynthesis from anoxygenic photosynthetic bacteria to gymnosperms. - J. Photochem. Photobiol. B 43: 87-100, 1998. Go to original source...
    4. Aro, E.M., Virgin, I., Andersson, B.: Photoinhibition of photosystem II. Inactivation, protein damage and turnover. - Biochim. Biophys. Acta 1143: 113-134, 1993. Go to original source...
    5. Beale, S.I.: Enzymes of chlorophyll biosynthesis. - Photosynth. Res. 60: 43-73, 1999. Go to original source...
    6. Canovas, F., McLarney, B., Silverthorne, J.: Light-independent synthesis of LHCIIb polypeptides and assembly of the major pigmented complexes during initial stages of Pinus palustris seedling development. - Photosynth. Res. 38: 89-97, 1993. Go to original source...
    7. Demko, V., Pavlovič, A., Valková, D. et al: A novel insight into the regulation of light-independent chlorophyll biosynthesis in Larix decidua and Picea abies seedlings. - Planta 230: 165-176, 2009. Go to original source...
    8. Demko, V., Pavlovič, A., Hudák, J.: Gabaculine alters plastid development and differentially affects abundance of plastidencoded DPOR and nuclear-encoded GluTR and FLU-like proteins in spruce cotyledons. - J. Plant Physiol. 167: 693-700, 2010. Go to original source...
    9. Domanskii, V., Rassadina, V., Gus-Mayer et al.: Characterization of two phases of chlorophyll formation during greening of etiolated barley leaves. - Planta 216: 475-483, 2003. Go to original source...
    10. DraŽić, G., Mihailović, N.: Chlorophyll accumulation in black pine seedlings treated with 5-aminolevulinic acid. - Biol. Plantarum 41: 277-280, 1998. Go to original source...
    11. DraŽić, G., Bogdanović, M.: Gabaculine does not inhibit cytokinin-stimulated biosynthesis of chlorophyll in Pinus nigra seedlings in the dark. - Plant Sci. 54: 23-29, 2000. Go to original source...
    12. Fujita, Y.: Protochlorophyllide reduction: a key step in the greening of plants. - Plant Cell Physiol. 37: 411-421, 1996. Go to original source...
    13. Fujita, Y., Bauer, C.E.: The light-independent protochlorophyllide reductase: A nitrogenase-like enzyme catalyzing a key reaction for greening in the dark. - In: Kadish, K.M., Smith, K.M., Guilard, R. (ed.): The Porphyrin Handbook. Vol. 13. Pp. 109-156. Elsevier Science, Amsterdam 2003. Go to original source...
    14. Gabruk, M., Grzyb, J., Kruk, J., Mysliwa-Kurdziel, B.: Lightdependent and light-independent protochlorophyllide oxidoreductases share similar sequence motifs - in silico studies. - Photosynthetica 50: 529-540, 2012. Go to original source...
    15. Granick, S.: Magnesium porphyrins formed by barley seedlings treated with 5-aminolevulinic acid. - Plant Physiol. 34 [Supl.]: XVIII, 1959.
    16. Griffiths, W.T.: Reconstitution of chlorophyllide formation by isolated etioplast membranes. - Biochem. J. 174: 681-692, 1978. Go to original source...
    17. Hideg, E., Kalai, T., Hideg, K., Vass, I.: Photoinhibition of photosynthesis in vivo results in singlet oxygen production detection via nitroxide-induced fluorescence quenching in broad bean leaves. - Biochemistry 37: 11405-11411, 1998. Go to original source...
    18. Hotta, Y., Tanaka, T., Takaoka, H., Takeuchi, Y., Konnai, M.: New physiological effects of 5-aminolevulinic acid in plants: The increased photosynthesis, chlorophyll content, and plant growth. - Biosci. Biotech. Bioch. 61: 2025-2028, 1997. Go to original source...
    19. Huang, L.Q., Castelfranco, P.A.: Regulation of 5-aminolevulinic acid synthesis in developing chloroplasts. I. Effect of light/dark treatments in vivo and in organello. - Plant Physiol. 90: 996-1002, 1989. Go to original source...
    20. Huang, L.Q., Bonner, B.A., Castelfranco, P.A.: Regulation of 5-aminolevulinic acid synthesis in developing chloroplasts. II. Regulation of ALA-synthesizing capacity by phytochrome. - Plant Physiol. 90: 1003-1008, 1989. Go to original source...
    21. Hudák, J., Gálová, E., Zemanová, L.: Plastid morphogenesis. - In: Pessarakli M. (ed.): Handbook of Photosynthesis. Pp. 221-245. CRC Press Taylor & Francis Group, Boca Raton, London, New York, Singapore 2005.
    22. Karpinska, B., Karpinski, S., Hällgren, J.E.: The chlB gene encoding a subunit of light-independent protochlorophyllide reductase is edited in chloroplasts of conifers. - Curr. Genet. 31: 343-347, 1997. Go to original source...
    23. Koski, V.M., Smith, J.H.C.: The isolation and spectral absorption properties of protochlorophyll from etiolated barley seedlings. - J. Amer. Chem. Soc. 70: 3558-3562, 1948. Go to original source...
    24. Lichtenthaler, H.K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. - Method. Enzymol. 148: 350-382, 1987. Go to original source...
    25. Mariani, P., De Carli, M.E., Rascio, N. et al.: Synthesis of chlorophyll and photosynthetic competence in etiolated and greening seedlings of Larix decidua as compared with Picea abies. - J. Plant Physiol. 137: 5-14, 1990. Go to original source...
    26. Masuda, T., Takamiya, K.: Novel insights into the enzymology, regulation and physiological functions of light-dependent protochlorophyllide oxidoreductase in angiosperms. - Photosynth. Res. 81: 1-29, 2004. Go to original source...
    27. Memon, S.A., Hou, X.L., Wang, L.J., Li, Y.: Promotive effect of 5-aminolevulinic acid on chlorophyll, antioxidative enzymes and photosynthesis of Pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee). - Acta Physiol. Plant. 31: 51-57, 2009. Go to original source...
    28. Mukai, Y., Tazaki, K., Fujii, T., Yamamoto, N.: Light-independent expression of three photosynthetic genes, cab, rbcS and rbcL, in coniferous plants. - Plant Cell Physiol. 33: 859-866, 1992.
    29. Nikolić, D., Bogdanović, M.: Plastid differentiation and chlorophyll synthesis in cotyledons of black pine seedlings grown in the dark. - Protoplasma 75: 205-213, 1972. Go to original source...
    30. Papenbrock, J., Grimm, B.: Regulatory network of tetrapyrrole biosynthesis - studies of intercellular signaling involved in metabolic and developmental control of plastids. - Planta 213: 667-681, 2001. Go to original source...
    31. Papenbrock, J., Mishra, S., Mock, H.P. et al.: Impaired expression of the plastidic ferrochelatase by antisense RNA synthesis leads to a necrotic phenotype of transformed tobacco plants. - Plant J. 28: 41-50, 2001. Go to original source...
    32. Pavlovič, A., Demko, V., Durchan, M., Hudák, J.: Feeding with aminolevulinic acid increased chlorophyll content in Norway spruce (Picea abies) in the dark. - Photosynthetica 47: 631-634, 2009. Go to original source...
    33. Peer, W., Silverthorne, J., Peters, J.L.: Developmental and lightregulated expression of individual members of light-harvesting complex b gene family in Pinus palustris. - Plant Physiol. 111: 627-634, 1996. Go to original source...
    34. Reinbothe, S. Reinbothe, C.: The regulation of enzymes involved in chlorophyll biosynthesis. - Eur. J. Biochem. 237: 323-343, 1996. Go to original source...
    35. Schendel, R., Dőrnemann, D., Rűdiger, W., Sineshchekov, V.: Comparative investigations of effect of 5-aminolevulinate feeding on phytochrome and protochlorophyll(ide) content in dark-grown seedlings of barley, cucumber and cress. - J. Photochem. Photobiol. B, 36: 245-253, 1996. Go to original source...
    36. Schoefs, B., Franck, F.: Protochlorophyllide reduction: Mechanism and evolution. - Photochem. Photobiol. 78: 543-557, 2003. Go to original source...
    37. Selstam, E., Widell, A., Johansson, L.B.Å.: A comparison of prolamellar bodies from wheat, Scots pine and Jeffrey pine - pigment spectra and properties of protochlorophyllide oxidoreductase. - Physiol. Plantarum 70: 209-214, 1987. Go to original source...
    38. Spano, A.J., He, Z., Timko, M.P.: NADPH:protochlorophyllide oxidoreductases in white pine (Pinus strobus) and loblolly pine (P. taeda). Evidence for light and developmental regulation of expression and conservation in gene organization and protein structure between angiosperms and gymnosperms. - Mol. Gen. Genet. 236: 86-95, 1992. Go to original source...
    39. Tanaka, R., Tanaka, A.: Tetrapyrrole biosynthesis in higher plants. - Annu. Rev. Plant Biol. 58: 321-346, 2007. Go to original source...
    40. Walles, B., Hudák, J.: A comparative study of chloroplast morphogenesis in seedlings of some conifers (Larix decidua, Pinu sylvestris and Picea abies). - Stud. Forest Suec. 127: 1-22, 1975.
    41. Wolwertz, M.R., Brouers, M.: Phototransformation of protochlorophyllideF657 in etiochloroplasts isolated from pine cotyledons; dark reformation of this pigment-complex from a pool of ALA-protochlorophyllideF635 in the presence of NADPH. - Photosynth. Res. 1: 105-113, 1980. Go to original source...
    42. Yamamoto, N., Mukai, Y., Matsuoka, M. et al.: Lightindependent expression of cab and rbcS genes in dark-grown pine seedlings. - Plant Physiol. 95: 379-383, 1991. Go to original source...

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