Photosynthetica 2018, 56(1):125-131 | DOI: 10.1007/s11099-017-0756-6

Fluorescence induction of photosynthetic bacteria

G. Sipka1,2, M. Kis1, J. L. Smart3, P. Maróti1,*
1 Department of Medical Physics, University of Szeged, Szeged, Hungary
2 Department of Plant Biology, Hungarian Academy of Science, Biological Research Centre, Szeged, Hungary
3 Department of Biological Sciences, University of Tennessee at Martin, Martin, USA

The kinetics of bacteriochlorophyll fluorescence in intact cells of the purple nonsulfur bacterium Rhodobacter sphaeroides were measured under continuous and pulsed actinic laser diode (808 nm wavelength and maximum 2 W light power) illumination on the micro- and millisecond timescale. The fluorescence induction curve was interpreted in terms of a combination of photochemical and triplet fluorescence quenchers and was demonstrated to be a reflection of redox changes and electron carrier dynamics. By adjustment of the conditions of single and multiple turnovers of the reaction center, we obtained 11 ms-1 and 120 μs-1 for the rate constants of cytochrome c23+ detachment and cyclic electron flow, respectively. The effects of cytochrome c2 deletion and chemical treatments of the bacteria and the advantages of the fluorescence induction study on the operation of the electron transport chain in vivo were discussed.

Additional key words: bacterial photosynthesis; electron transfer; fluorescence transients; intact cells

Received: May 16, 2017; Accepted: August 28, 2017; Published: March 1, 2018  Show citation

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Sipka, G., Kis, M., Smart, J.L., & Maróti, P. (2018). Fluorescence induction of photosynthetic bacteria. Photosynthetica, 56(SPECIAL ISSUE), 125-131. doi: 10.1007/s11099-017-0756-6
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