Photosynthetica 2018, 56(1):132-138 | DOI: 10.1007/s11099-018-0780-1

Application of spectrally resolved fluorescence induction to study light-induced nonphotochemical quenching in algae

R. Kaňa1,*
1 Institute of Microbiology, Czech Academy of Sciences, Třeboň, Czech Republic

The light-induced nonphotochemical quenching (NPQ) can safely dissipate excess of absorbed light to heat. Here we describe an application of spectrally resolved fluorescence induction (SRFI) method for studying spectral variability of NPQ. The approach allows detection of spectrally-resolved nonphotochemical quenching (NPQλ) representing NPQ dependency on fluorescence emission wavelength in the whole spectral range of fluorescence emission. The experimental approach is briefly described and NPQλ is studied for the cryptophyte alga Rhodomonas salina and for green alga Chlorella sp. We confirm presence of NPQλ only in membrane-bound antennae (chlorophyll a/c antennae) and not in phycobiliproteins in lumen in cryptophyte and show that NPQλ is inhibited in the whole spectral range by NPQ inhibitors in Chlorella sp. We discuss variability in the quenching in the particular spectral ranges and applicability of the NPQλ parameter to study quenching locus in vivo.

Additional key words: fluorescence parameters; light-harvesting complex; photoprotection; photosynthesis; photosystem II

Received: May 19, 2017; Accepted: October 10, 2017; Published: March 1, 2018  Show citation

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Kaňa, R. (2018). Application of spectrally resolved fluorescence induction to study light-induced nonphotochemical quenching in algae. Photosynthetica, 56(SPECIAL ISSUE), 132-138. doi: 10.1007/s11099-018-0780-1
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